LOCUS       sequence-178854-        5104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector at an attB site in Bacteroides; contains the 
            P1T_DP aTC-inducible promoter with the RBS A21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Lim B, Zimmermann M, Barry NA, Goodman AL
  TITLE     Engineered Regulatory Systems Modulate Gene Expression of Human 
            Commensals in the Gut.
  JOURNAL   Cell. 2017 Apr 20;169(3):547-558.e15. doi: 
            10.1016/j.cell.2017.03.045.
  PUBMED    28431252
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20- 
            20"; volume: "169"; issue: "3"; pages: "547-558.e15"
COMMENT     Sequence Label: pNBU2_erm_P1T_DP-A21
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            102..211
                     /note="incP origin of transfer"
     rep_origin      382..770
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    885..903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    947..965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(4215..4283,4284..5075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4433..4452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caattcgagg gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
       61 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      121 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      181 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      241 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
      301 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc
      361 cacgatgcgt ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta
      421 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
      481 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
      541 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
      601 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
      661 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
      721 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc
      781 cctccaccgc ggtggctttt gcacccgctt tccaagagaa gaaagccttg ttaaattgac
      841 ttagtgtaaa agcgcagtac tgcttgacca taagaacaaa aaaatctcta tcactgatag
      901 ggataaagtt tggaagataa agctaaaagt tcttatcttt gcagtctccc tatcagtgat
      961 agagacgcat tttaaaataa aataaattat ttatgatatt aaacgaatcc atggtggatc
     1021 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgactaat tgcctatctt
     1081 ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc
     1141 tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc
     1201 cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga
     1261 aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc
     1321 cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac
     1381 cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttt ttcctgcatg
     1441 ggtactgagc aaagcgtatt tgggtgtgac ttcatcaata cgttcatttc ccttgtagta
     1501 ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt
     1561 catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta
     1621 tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt
     1681 gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc
     1741 cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta
     1801 ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa
     1861 gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg
     1921 atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc
     1981 gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc
     2041 aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac
     2101 tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac
     2161 aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt cttcactggt
     2221 gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg
     2281 cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt
     2341 gatttcggct gcactttgct ttagcttgtt ggtacatccg ttctttaccc gctgcttatc
     2401 tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatac gttggctggc
     2461 aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg
     2521 gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc
     2581 tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt
     2641 tactctatat tgtaatttac acttgattat cagtcgtttg cagttttatg atattctgtg
     2701 aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa
     2761 aacaaacacc ctgttttaca cccaagaatg taaagtcggg tgtttttgtt ttatttaaga
     2821 taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac
     2881 taatatttat ctatccattc agtttgattt ctcaggactt tacatcgtcc tgaaagtatt
     2941 tgttggtacc gaggacgcgt aaacatttac agttgcatgt ggcctattgt ttttagccgt
     3001 taaatatttt ataactatta aatagcgata caaattgttc gaaactaata ttgtttatat
     3061 catatattct cgcatgtttt aaagctttat taaattgatt ttttgtaaac agtttttcgt
     3121 actctttgtt aacccatttc attacaaaag tttcatattt ttttctctct ttaaatgcca
     3181 tttttgctgg ctttcttttt aatacaatta atgtgctatc cactttaggt tttggatgga
     3241 aataatacct aggaattttt gctaatatag aaatatctac ctctgccatt aacagcaatg
     3301 ctagtgatct gtttgtatct aataacattt tagcaaaacc atattccact attaaataac
     3361 ttattgtggc tgaactttca aaaacaattt ttcgaattat atttgtgctt atgttgtaag
     3421 gtatgctgcc aaatatttta tatggattgt ggctaggaaa tgtaaatttc agtatatcat
     3481 catttactat ttgatagtta ggataattta agagcttatt acgagttacc tcacataatt
     3541 tagaatcaat ttctatcgcc gttacaaaat tacatctctt taccaatcca gcagtaaaat
     3601 gacctttccc tgcacctatt tcaaagatgt tatctttttc atctaaactt atgcaattca
     3661 ttattttttc tatgtgatat tttgaagtaa taaaattttg actatctttt atatttactt
     3721 tgttcattat aacctctcct taatttattg catctctttt cgaatattta tgttttttga
     3781 gaaaagaacg tactcatggt tcatcccgat atgcgtatcg gtctgtatat cagcaacttt
     3841 ctatgtgttt caactacaat agtcatctat tctcatcttt ctgagtccac cccctgcaaa
     3901 gcccctcttt acgacataaa aattcggtcg gaaaaggtat gcaaaagatg tttctctctt
     3961 taagagaaac tcttcgggat gcaaaaatat gaaaataact ccaattcacc aaattatata
     4021 gcgacttttt tacaaaatgc taaaatttgt tgatttccgt caagcaattg ttgagcaaaa
     4081 atgtctttta cgataaaatg atacctcaat atcaactgtt tagcaaaacg atatttctct
     4141 taaagagaga aacacctttt tgttcaccaa tccccgactt ttaatcccgc ggccatgatt
     4201 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4321 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4381 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4441 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4501 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcataac
     5101 gcgt
//
