LOCUS       sequence-178875-        8808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of copGFP-T2A-FLuc under control of a 
            COL2A1-based, chondrogenesis-responsive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   De La Vega RE, Scheu M, Brown LA, Evans C, Ferreira E, Porter RM
  TITLE     Specific, sensitive and stable reporting of human mesenchymal 
            stromal cell chondrogenesis.
  JOURNAL   Tissue Eng Part C Methods. 2019 Feb 6. doi: 
            10.1089/ten.TEC.2018.0295.
  PUBMED    30727864
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Tissue Eng 
            Part C Methods. 2019 Feb 6. doi: 10.1089/ten.TEC.2018.0295."
COMMENT     Sequence Label: pGF1-4eCOL2A1
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2356..3021
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2356..3021
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             3163..3216
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3223..4875
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3289..3307)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    4886..5474
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4939..4959)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5357..5368)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5565..5798
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5870..5991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5907..5926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5961..5980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6010..6145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6183..6199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6183..6199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6196..6218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6207..6223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6231..6237,6238..6255,6256..6261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6276..6297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6414..6431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6585..7173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6665..6684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7344..8135,8136..8204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7967..7986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8205..8309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8377..8395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     8555..8574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8768..8790
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8782..8799
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8783..8799
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatgaat tctagaacta gtctgtgaat cgggctctgt gtgcgctcga gaaaagcccc
     1981 attcatgaga gctgtgaatc gggctctgtg tgcgctcgag aaaagcccca ttcatgagag
     2041 ctgtgaatcg ggctctgtgt gcgctcgaga aaagccccat tcatgagagc tgtgaatcgg
     2101 gctctgtgtg cgctcgagaa aagccccatt catgagagct agccggcttg ggcaggtgcg
     2161 ggcactggca gggcccaggc gggctccggg ggcgggcggt tcaggttaca gcccagctgg
     2221 gggcaggggg cggcccgcgg tttgggcgag ttcgccagcc tcgaaagggg ccgggcgcat
     2281 ataacgggcg ccgcggcggg gagaagacgc agagcgctgc tgggctgccg ggtctcccgc
     2341 ttcggatccg ccaccatgcc cgccatgaag atcgagtgcc gcatcaccgg caccctgaac
     2401 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc
     2461 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     2521 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     2581 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     2641 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     2701 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     2761 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     2821 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     2881 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     2941 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     3001 aagaccccca tcgccttcgc cagatctcga gatatcagcc atggcttccc gccggcggtg
     3061 gcggcgcagg atgatggcac gctgcccatg tcttgtgccc aggagagcgg gatggaccgt
     3121 caccctgcag cctgtgcttc tgctaggatc aatgtgaccg gtgagggcag aggaagtctt
     3181 ctaacatgcg gtgacgtgga ggagaatccc ggcccttccg gtatggaaga cgccaaaaac
     3241 ataaagaaag gcccggcgcc attctatccg ctagaggatg gaaccgctgg agagcaactg
     3301 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3361 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
     3421 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3481 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3541 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
     3601 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
     3661 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3721 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
     3781 gatcgtgaca aaacaattgc actgataatg aactcctctg gatctactgg gttacctaag
     3841 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
     3901 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     3961 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     4021 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
     4081 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
     4141 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
     4201 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
     4261 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     4321 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
     4381 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
     4441 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4501 gacgaagacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
     4561 taccaggtgg cccccgctga attggagtcg atattgttac aacaccccaa catcttcgac
     4621 gcgggcgtgg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4681 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4741 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     4801 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     4861 aagtccaaat tgtaatcgac tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4921 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4981 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5041 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5101 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5161 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5221 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5281 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5341 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5401 ggctctgcgg cctcttccgc atcttcgcct tcgccctcag acgagtcgga tctccctttg
     5461 ggccgcctcc ccgcctggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5521 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5581 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5641 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5701 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5761 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5821 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     5881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5941 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6001 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6061 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6121 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     6181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7021 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7201 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8341 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8401 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8461 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8521 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8581 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8641 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8701 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8761 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
