LOCUS       sequence-178877-        8269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains yrk enhancer sequence, and yrk specific gene promoter 
            driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;yrkCE1-P1Egfp
FEATURES             Location/Qualifiers
     source          1..8269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     623..639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    659..679
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1548..1572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2271..2280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2277..2279,2280..2282,2283..2993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2277..2279,2280..2282,2283..2993)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2322..2343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2583..2602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2930..2951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2994..3059
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(3080..3104)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3130..3150)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3158..3174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3190..3324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(3763..3950,3951..3962)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3996..4012)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3996..4012)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4049..4069)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4049..4067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4088..4104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4101..4123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4112..4128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4136..4142,4143..4160,4161..4166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4181..4202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4319..4336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4490..5078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4570..4589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5249..6040,6041..6109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5872..5891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6110..6214)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6377..6398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6747..6758,6759..6921)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7290..7411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7534..8244)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(7614..7637)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7812..7831)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8081..8099
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8241..8250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtaactcct tactgtaact tattgcacct gatgaacaaa agatgtgaac tgaacgcaag
       61 taggaggcta atagaggcta gtacagtaca aagatcccca aatgatgtgt gccggcctta
      121 gtggaggcag acccctggtt atatacctgt gctgcagaaa gaggggctta caaatggatc
      181 tctctggaat ggcattatct catggtggga acagtgttgt cagcagaatc agcggggtgg
      241 actctgacct gagaatgaca gtctagcact gaatggctca gacaatactc ttgtggccaa
      301 ctatcaccgg aaaataagtg cctgccaaaa caaaccagac cagatgaagc tacatggctg
      361 tagaacgtca gtatttaatg tgttgtggcc acttggttca ccagatttaa gagctaattt
      421 ttttaaatat cacagataat caatcgcttt tttttttctt gactgaaaag cttagactgg
      481 aaaattcttt gaaaaagttt gcactacaga aaataaatac ataacatcct gcactgaatg
      541 cacactatta atagaaaaga gagcattatt gtaggaaatg tattgtatat gtacattctg
      601 tgcatttcac aattttctcg agcaggaaac agctatgacc atgattacgc caagctatca
      661 actttgtata gaaaagttgc gttctcagta ctttaaagag cctatgacaa caacaacaac
      721 aacaacaaca tcatgcatat ttaatgtcac agtatattgc agcattgcat actggactaa
      781 aaagcatgat gaggtttgat taattaaatg gaaaacagaa tggaatgtct ctaggactag
      841 attaggccat aggcttcatc acttcctgtt ctgtgcaagg tcacttcctg tctactctcc
      901 cctggcgcta tgggaggaag tgggtgcggc tgtccggtta aattgtccga aatgcaacat
      961 ggttaaactg accaaaccag agcctgcaat gtggattttc agctctccga tgttctgttc
     1021 actactgacc tgatgtttag tgtgtaggaa acgttggcta aatcccacat attgtattca
     1081 atgggttgct tgtttgatta gcgtgaaact ggcattgatt ctgatgatga atacattttt
     1141 ttggctttag ttttatccaa ttagtgcatg catattcaga gaaagtaagt ttaaaaagca
     1201 catatattta gtttattttc cgaaagcacc cgtgctgaaa tactgcgttc attaacggcc
     1261 tgctcgtgga atctggctgt ggaggaaatt gacatcatgt ggaaaaagga agtggctgtg
     1321 tttgtgacac ctgactttct gttcccaggg cacactctgc tattgtcttc cctctctgca
     1381 atattatctc tcagtttctc tctcactgca ttgtttcacc tagttgttgt tgttgttgtt
     1441 tatttgcctg agtgactttt attacagttt ttatgagtgt gtgggtgtat tcagcatggt
     1501 ctcacatttt cctgttgtga taattgctaa atctttaatc agggtataca agtttgtaca
     1561 aaaaagcagg ctcgctacag attaaagatt ggagttttaa actcttatgt gcatttttat
     1621 cattctattt tatataataa tatttaataa taataataat aataactggc tttaaataaa
     1681 tacacaaggg attatttatt aatttattat gattcattaa ttaattagat aaatgcaatt
     1741 tatgtattcc ttccctttat actatgtatg tatcttaaaa tggtagtatt tattataatt
     1801 attttattta attgattaaa acatttatca aacatgcaga actgtccttc cccaaaacag
     1861 ccactaggga ccggaaatgt acaacaaaca ttttcaggca ccagttaaca tcacctaaca
     1921 ccctcacatg tacctgacag tactgtacat aactattgaa aagttgtctg aatattgttc
     1981 ggcactatta atatacctgc agtcggctac gaatgatatt cctctccaac atcaacacaa
     2041 agtaagccat gaaacttcac cgtcacaatt acgtggtgaa accgcgttga atatttagca
     2101 aataatgcaa cttcccggaa atacattgat gggctgcatt tgttttaagc agagaggagg
     2161 cggagttcag cgtttccgga gagcgcgcgc ctgaagcagc gcatcgcggc cagttgatcg
     2221 ccgctcggac actcaagctc cgccaccctg cctcctccag cgacattatc gccaccatgg
     2281 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2341 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2401 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2461 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2521 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2581 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2641 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2701 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2761 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2821 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2881 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2941 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
     3001 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     3061 ccggatctag ataactgata cccagctttc ttgtacaaag tggggcgcgc ctacgtaact
     3121 agtctcgagc aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc
     3181 atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     3421 gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
     3481 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     3541 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     3601 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
     3661 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
     3721 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     3781 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     3841 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     3901 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     3961 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
     4021 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     4081 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4141 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4201 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4261 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc atgagattat
     5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     6241 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     6301 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     6361 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     6421 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa gaactcatca
     6481 gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg acggctacta
     6541 catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca acaatgctct
     6601 tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag aaatgccctc
     6661 tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg
     6721 tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca
     6781 agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta
     6841 gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg
     6901 aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa
     6961 aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt
     7021 atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc
     7081 agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg
     7141 gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga
     7201 ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg
     7261 gtgtctgaaa cacaggccca tttacgcctt aagatacatt gatgagtttg gacaaaccac
     7321 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7381 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7441 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     7501 tatggctgat tatgatctag agtcgcggcc gctttacttg tacagctcgt ccatgccgcc
     7561 ggtggagtgg tggccctcgg cgcgctcgta ctgttccacg atggtgtagt cctcgttgtg
     7621 ggaggtgatg tccagcttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt
     7681 ggccttgtag gtggtcttga cctcggcgtc gtagtggccg ccgtccttca gcttcagcct
     7741 ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc
     7801 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
     7861 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
     7921 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
     7981 caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg
     8041 aggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc
     8101 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     8161 gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt
     8221 gtcatcctcg cccttgctca ccatggtggc gaccggtaat gtcagacct
//
