LOCUS       sequence-17888-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Medema RH, Herrera RE, Lam F, Weinberg RA
  TITLE     Growth suppression by p16ink4 requires functional retinoblastoma 
            protein.
  JOURNAL   Proc Natl Acad Sci U S A. 1995 Jul 3. 92(14):6289-93.
  PUBMED    7603984
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1995 Jul 3. 92(14):6289-93."
COMMENT     Sequence Label: pCMV p16 INK4A
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(508..526)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(509..526)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(546..563)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    552..776
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacggccg ccagtgtgct ggaattcacc accatggagc cttcggctga ctggctggcc
       61 acggccgcgg cccggggtcg ggtagaggag gtgcgggcgc tgctggaggc gggggcgctg
      121 cccaacgcac cgaatagtta cggtcggagg ccgatccagg tcatgatgat gggcagcgcc
      181 cgagtggcgg agctgctgct gctccacggc gcggagccca actgcgccga ccccgccact
      241 ctcacccgac ccgtgcacga cgctgcccgg gagggcttcc tggacacgct ggtggtgctg
      301 caccgggccg gggcgcggct ggacgtgcgc gatgcctggg gccgtctgcc cgtggacctg
      361 gctgaggagc tgggccatcg cgatgtcgca cggtacctgc gcgcggctgc ggggggcacc
      421 agaggcagta accatgcccg catagatgcc gcggaaggtc cctcagatat ccccgattga
      481 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg
      541 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
      601 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
      661 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
      721 gggggaggat tgggaagaca atagcaagca tgctggggat gcggtgggct ctatggcttc
      781 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct g
//
