LOCUS       sequence-178895-        8554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral gene expression vector for MEF2C-S222A expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: MSCV-IRES-Tomato MEF2C S222A
FEATURES             Location/Qualifiers
     source          1..8554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1421..1430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2871..3438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2888..3440
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3032..3049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3259..3278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3452..4882
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     LTR             4947..5461
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5623..5639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5647..5653,5654..5671,5672..5677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5692..5713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5830..5847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6001..6589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6081..6100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6760..7551,7552..7620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7383..7402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7621..7725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7793..7811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7849..7871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7971..7990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8184..8206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8334..8353
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8553..8554,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga gccaccatgg ggagaaaaaa
     1441 gattcagatt acgaggatta tggatgaacg taacagacag gtgacattta caaagaggaa
     1501 atttgggttg atgaagaagg cttatgagct gagcgtgctg tgtgactgtg agattgcgct
     1561 gatcatcttc aacagcacca acaagctgtt ccagtatgcc agcaccgaca tggacaaagt
     1621 gcttctcaag tacacggagt acaacgagcc gcatgagagc cggacaaact cagacatcgt
     1681 ggagacgttg agaaagaagg gccttaatgg ctgtgacagc ccagaccccg atgcggacga
     1741 ttccgtaggt cacagccctg agtctgagga caagtacagg aaaattaacg aagatattga
     1801 tctaatgatc agcaggcaaa gattgtgtgc tgttccacct cccaacttcg agatgccagt
     1861 ctccatccca gtgtccagcc acaacagttt ggtgtacagc aaccctgtca gctcactggg
     1921 aaaccccaac ctattgccac tggctcaccc ttctctgcag aggaatagta tgtctcctgg
     1981 tgtaacacat cgacctccaa gtgcaggtaa cacaggtggt ctgatgggtg gagacctcac
     2041 gtctggtgca ggcaccagtg cagggaacgg gtatggcaat ccccgaaacg caccaggtct
     2101 gctggtctca cctggtaact tgaacaagaa tatgcaagca aaatctcctc ccccaatgaa
     2161 tttaggaatg aataaccgta aaccagatct ccgagttctt attccaccag gcagcaagaa
     2221 tacgatgcca tcagtgtctg aggatgtcga cctgcttttg aatcaaagga taaataactc
     2281 ccagtcggct cagtcattgg ctaccccagt ggtttccgta gcaactccta ctttaccagg
     2341 acaaggaatg ggaggatatc catcagccat ttcaacaaca tatggtaccg agtactctct
     2401 gagtagtgca gacctgtcat ctctgtctgg gtttaacacc gccagcgctc ttcaccttgg
     2461 ttcagtaact ggctggcaac agcaacacct acataacatg ccaccatctg ccctcagtca
     2521 gttgggagct tgcactagca ctcatttatc tcagagttca aatctctccc tgccttctac
     2581 tcaaagcctc aacatcaagt cagaacctgt ttctcctcct agagaccgta ccaccacccc
     2641 ttcgagatac ccacaacaca cgcgccacga ggcggggaga tctcctgttg acagcttgag
     2701 cagctgtagc agttcgtacg acgggagcga ccgagaggat caccggaacg aattccactc
     2761 ccccattgga ctcaccagac cttcgccgga cgaaagggaa agtccctcag tcaagcgcat
     2821 gcgactttct gaaggatggg caacataagg atcccccggg ctgcaggaat tccgcccccc
     2881 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2941 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     3001 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     3061 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     3121 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     3181 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     3241 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     3301 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     3361 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     3421 tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg aggtcatcaa
     3481 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     3541 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     3601 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     3661 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     3721 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     3781 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     3841 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     3901 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     3961 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     4021 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     4081 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     4141 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     4201 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     4261 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4321 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     4381 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     4441 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     4501 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     4561 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     4621 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     4681 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     4741 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     4801 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     4861 catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataaaa taaaagattt
     4921 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     4981 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     5041 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     5101 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     5161 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     5221 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     5281 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     5341 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     5401 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     5461 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
     5521 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
     5581 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
     5641 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5701 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5761 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     7861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     7921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     7981 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     8041 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     8101 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     8161 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
     8221 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     8281 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     8341 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     8401 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     8461 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
     8521 aagattttat ttagtctcca gaaaaagggg ggaa
//
