LOCUS       sequence-17892-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Liu X, Sun Y, Constantinescu SN, Karam E, Weinberg RA, Lodish HF
  TITLE     Transforming growth factor beta-induced phosphorylation of Smad3 is 
            required for growth inhibition and transcriptional induction in 
            epithelial cells.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Sep 30. 94(20):10669-74.
  PUBMED    9380693
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Sep 30. 94(20):10669-74."
COMMENT     Sequence Label: pEXL flag Smad3
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        223..241
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     223..240
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     254..273
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    322..374
                     /label=MCS
                     /note="multiple cloning site"
     CDS             413..436
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      121 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
      181 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      241 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
      301 ggttctatcg attgaattga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
      361 cgggcccggg atcccagtgt ggtggtacgg gaattcgcgt atcgatacca tggactacaa
      421 ggacgacgat gacaagggtc gactcatgtc gtccatcctg cctttcactc ccccgatcgt
      481 gaagcgcctg ctgggctgga agaagggcga gcagaacggg caggaggaga aatggtgcga
      541 gaaggcggtc aagagcctgg tcaagaaact caagaagacg gggcagctgg acgagctgga
      601 gaaggccatc accacgcaga acgtcaacac caagtgcatc accatcccca ggtccctgga
      661 tggccggttg caggtgtccc atcggaaggg gctccctcat gtcatctact gccgcctgtg
      721 gcgatggcca gacctgcaca gccaccacga gctgcgggcc atggagctgt gtgagttcgc
      781 cttcaatatg aagaaggacg aggtctgcgt gaatccctac cactaccaga gagtagagac
      841 accagttcta cctcctgtgt tggtgccacg ccacacagag atcccggccg agttcccccc
      901 actggacgac tacagccatt ccatccccga aaac
//
