LOCUS       sequence-178927-        2414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, 
            Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang
            P, Sun Q, Shi L, Yang H
  TITLE     Homology-mediated end joining-based targeted integration using 
            CRISPR/Cas9.
  JOURNAL   Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
  PUBMED    28524166
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2017 May 19. doi: 10.1038/cr.2017.76."
COMMENT     Sequence Label: AAV_Cdx2 HMEJ donor
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(549..560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             824..880
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             881..1591
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1026..1044)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1294..1313
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agaccacggg aggggtcact gggtgattgc aggggacacc cagtgcctct ggcttctgtg
       61 gatatatatg aatgtataca gacatacaca tatcaaagat ttaaaaatcc ttgaactttt
      121 tttttcatta acacaaaaga ggccctactt gcacaccaac actttctgta gtctagggaa
      181 aagggaagga acaaacaaga gaaattcata gccaggaagt tttggacaaa gtttcattgt
      241 ctgtgccaag gtgaagaaca tcgcagtacc aatgagctgt gggagcccaa gggtgaccca
      301 cgaggcacac tgagctggcc aaccatttgt gtagggaata gctttcgtca tggttccgtt
      361 ccctggttct gaggttctgt tgctagtaaa gggttttttg tttgtttgtt ttttgttttt
      421 tgaaagcaac ttggggaggg ggcaggtaag taaagaggac gaggctggcc tggctttggg
      481 aacatttccc aaactcagtg aaagaaggac gctcccgggg tggcgccatt tctctgttct
      541 tatcttcttc taccttctat tttgtcatct ctaggttaaa atttggtttc agaaccgcag
      601 agccaaggag aggaaaatca agaagaagca gcagcagcaa cagcagcagc agcaacaaca
      661 gcctccacag ccgccgccac aaccttccca gcctcagccg ggtgccctgc ggagcgtgcc
      721 cgagcccttg agtcctgtga cctccttgca aggctcagtg cctggttctg tccctggggt
      781 tctggggcca gctggagggg ttttaaactc cactgtcacc caggccacta acttctccct
      841 gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca atggtgagca agggcgagga
      901 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      961 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     1021 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     1081 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     1141 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     1201 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     1261 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     1321 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     1381 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     1441 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     1501 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     1561 caccggcggc atggacgagc tgtacaagta agacccctcc cgtggtctga agcggcggcg
     1621 gcacagcaat cccaggctga gccatgagga gtatggacgc tgcgagaatc ctcagaagag
     1681 attcctctcc tcctacccac gaacagcatc tactgatgga gattgaggac agaagatgag
     1741 tggaattatg gacctcaggg gaagacatgg tttagatttt ttttttcttt ttaacttttc
     1801 ccattccgac tcttcctgcc agcaacgaca aacgaagtga ttcctggggc ttcttcgttc
     1861 atgctctttg ccaggactga ctaccgacat gaagctatca gcctcttttg ccccagctct
     1921 ttgcctctct gtatttctgt gtggagctga ggagagagtg agactggatg gggtgggggt
     1981 agcaatactt gagccaaggt ggctgtttcc tgctgactgc tttctgagaa ccagctggcc
     2041 gtcctgcctc cgggccaggg actattcaaa ctacaggagc cagaggcagc taagatagct
     2101 ggactgaccg aagtctgcag aacctccccc accaggtggt ctgggctttc ttctccacaa
     2161 atcaggaagg ggtggtgggt tcaggggctg cggtgagagg gggttggtta gccaacgcca
     2221 ggcccctgcg acaagggctt gtttagaaag cctgtcacca gagctgctgt aggcggaatg
     2281 tatgtctgtg ttgtaaatgc cagagccaac ctggacttcc tgtcccttcc ctcgtctttg
     2341 gctgaagaag accggaattg tttgctgctg ttcgagtcac tgatctgtgt acccagtgac
     2401 ccctcccgtg gtct
//
