LOCUS       sequence-17893-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p138
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    38..71
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(107..310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(136..156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        311..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gaagctctcg cccttgctcc catggtggcg accggtgata acttcgtata atgtatgcta
       61 tacgaagtta tcggtaccgc tagcggatct gacggttcac taaaccagct ctgcttatat
      121 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      181 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      241 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      301 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      361 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      421 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      481 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
      541 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      601 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
      661 actattaata actaatgcat ggcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      781 ctggcgtttt tccataggct ccgccccccc tgacgagcat cacaaaaatc gacgctcaag
      841 tcagaggtgg cgaaacccga caggactata aagataccag gcgttttccc ccctgg
//
