LOCUS       sequence-178933-        2431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Yao X, Wang X, Hu X, Liu Z, Liu J, Zhou H, Shen X, Wei Y, Huang Z, 
            Ying W, Wang Y, Nie YH, Zhang CC, Li S, Cheng L, Wang Q, Wu Y, Huang
            P, Sun Q, Shi L, Yang H
  TITLE     Homology-mediated end joining-based targeted integration using 
            CRISPR/Cas9.
  JOURNAL   Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
  PUBMED    28524166
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2017 May 19. doi: 10.1038/cr.2017.76."
COMMENT     Sequence Label: AAV_Sox2 HMEJ donor
FEATURES             Location/Qualifiers
     source          1..2431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             833..889
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             890..1600
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1035..1053)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1303..1322
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcccctgtc gcacatgtga gggggtcccg ggggcagcgg cgtaagatgg cccaggagaa
       61 ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gcggagtgga aacttttgtc
      121 cgagaccgag aagcggccgt tcatcgacga ggccaagcgg ctgcgcgctc tgcacatgaa
      181 ggagcacccg gattataaat accggccgcg gcggaaaacc aagacgctca tgaagaagga
      241 taagtacacg cttcccggag gcttgctggc ccccggcggg aacagcatgg cgagcggggt
      301 tggggtgggc gccggcctgg gtgcgggcgt gaaccagcgc atggacagct acgcgcacat
      361 gaacggctgg agcaacggca gctacagcat gatgcaggag cagctgggct acccgcagca
      421 cccgggcctc aacgctcacg gcgcggcaca gatgcaaccg atgcaccgct acgacgtcag
      481 cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct cgcccaccta
      541 cagcatgtcc tactcgcagc agggcacccc cggtatggcg ctgggctcca tgggctctgt
      601 ggtcaagtcc gaggccagct ccagcccccc cgtggttacc tcttcctccc actccagggc
      661 gccctgccag gccggggacc tccgggacat gatcagcatg tacctccccg gcgccgaggt
      721 gccggagccc gctgcgccca gtagactgca catggcccag cactaccaga gcggcccggt
      781 gcccggcacg gccattaacg gcacactgcc cctgtcgcac atgttaatta acgccactaa
      841 cttctccctg ttgaaacaag caggggatgt cgaagagaat cccgggccaa tggtgagcaa
      901 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      961 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1021 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     1081 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     1141 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1201 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     1261 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     1321 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     1381 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     1441 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     1501 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     1561 ccgccactcc accggcggca tggacgagct gtacaagtaa ggcgcgcctg agggctggac
     1621 tgcgaactgg agaaggggag agattttcaa agagatacaa gggaattggg aggggtgcaa
     1681 aaagaggaga gtaggaaaaa tctgataatg ctcaaaagga aaaaaaatct ccgcagcgaa
     1741 acgacagctg cggaaaaaaa ccaccaatcc catccaaatt aacgcaaaaa ccgtgatgcc
     1801 gactagaaaa cttttatgag agatcttggg acttcttttt gggggactat ttttgtacag
     1861 agaaaacctg agggcggcgg ggagggcggg ggaatcggac catgtataga tctggaggaa
     1921 aaaaactacg caaaactttt ttttaaagtt ctagtggtac gttaggcgct tcgcagggag
     1981 ttcgcaaaag tctttaccag taatatttag agctagactc cgggcgatga aaaaaaagtt
     2041 ttaatatttg caagcaactt ttgtacagta tttatcgaga taaacatggc aatcaaatgt
     2101 ccattgttta taagctgaga atttgccaat atttttcgag gaaagggttc ttgctgggtt
     2161 ttgattctgc agcttaaatt taggaccgtt acaaacaagg aaggagttta ttcggatttg
     2221 aacattttag ttttaaaatt gtacaaaagg aaaacatgag agcaagtact ggcaagaccg
     2281 ttttcgtggt cttgtttaag gcaaacgttc tagattgtac taaattttta acttactgtt
     2341 aaaggcaaaa aaaaaatgtc catgcaggtt gatatcgttg gtaatttata atagcttttg
     2401 ttcaatcccc ctcacatgtg cgacaggggc a
//
