LOCUS       sequence-178950-        5575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6 wt in pSE358 (TRP1 CEN).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Madhani HD, Bordonne R, Guthrie C
  TITLE     Multiple roles for U6 snRNA in the splicing pathway.
  JOURNAL   Genes Dev. 1990 Dec;4(12B):2264-77.
  PUBMED    2149118
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "1990-12"; volume: "4(12B)"; pages: "2264-77"
COMMENT     Sequence Label: pSX6
FEATURES             Location/Qualifiers
     source          1..5575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(671..688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(671..687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1202..1494
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      1495..1948
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(1787..2461)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2462..2563)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(2580..2598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2666..2770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2771..2839,2840..3631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2989..3008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3802..4390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4291..4310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4544..4561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4678..4699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4714..4719,4720..4737,4738..4744)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4752..4768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4757..4779
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4776..4792
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4776..4792
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttcgcgaag taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag
       61 cagttcccct gcataaggat gaaccgtttt acaaagagat ttatttcgtt ttttttttat
      121 cctcgagtga agtatcgtga cttgtacatt tgaagatacc cagcgtacag cagtgtatct
      181 ttatcttcct gtatgatata gataactaac atctcgaata gaaaattgtc tcgcgttcga
      241 acctaagcct ttgctatcgg ttgaacatag aagaccgcgt gattcatttt agtgcgtaag
      301 aatgctatta gtacttatgt gctttatgaa tgtgaatatt gttacagcta tgcaatggta
      361 cgatcatact ctgaacgatc catttaaatt atatatgtca aacagaagag aaaggttttt
      421 ttattgaaga tttcacagaa aatcaatcat tttgcaatat tccttcgtcc cagagaaggt
      481 ttcggctttt acagcaacga gaatgattct tccattattt gtacctagct tccacatgga
      541 tttcgataag tgtagtttca aaccatgaac gcaattcaaa actttcaaat tggtgcaatc
      601 caagaccctc cctttacatt tggagtcgtt atcaagctta tcgataccgt cgacggatcc
      661 gaagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      721 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      781 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      841 attttctcct tacgcatctg tgcggtattt cacaccgcat aactagcttt taacttgtat
      901 cctaggttat ctatgctgtc tcaccataga gaatattacc tatttcagaa tgtatgtcca
      961 tgattcgccg ggtaaataca tataatacac aaatctggct taataaagtc tataatatat
     1021 ctcataaaga agtgctaaat tggctagtgc tatatatttt taagaaaatt tcttttgact
     1081 aagtccatat cgactttgta aaagttcaca ttagcataca tatattacac gagccagaaa
     1141 tagtaacttt tgcctaaatc acaaattgca aaatttaatt gcttgcaaaa ggtcacatgc
     1201 ttataatcaa cttttttaaa aatttaaaat acttttttat tttttatttt taaacataaa
     1261 tgaaataatt tatttattgt ttatgattac cgaaacataa aacctgctca agaaaaagaa
     1321 actgttttgt ccttggaaaa aaagcactac ctaggagcgg ccaaaatgcc gaggctttca
     1381 tagcttaaac tctttacaga aaataggcat tatagatcag ttcgagtttt cttattcttc
     1441 cttccggttt tatcgtcaca gttttacagt aaataagtat cacctcttag agttggcggt
     1501 tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg
     1561 aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc
     1621 gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat
     1681 cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca
     1741 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat
     1801 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc
     1861 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca
     1921 gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca acccagtcag
     1981 aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca aacaagggaa
     2041 taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct tttggaaata
     2101 cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac gactcatctc
     2161 catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca tcctccttag
     2221 gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt ttatatgctt
     2281 ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt ctattgggca
     2341 cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg gcctctgtgc
     2401 tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta ataacagaca
     2461 tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat agtcaccaat
     2521 gccctccctc ttggccctct ccttttcttt tttcgaccga atttcttgaa gacgaaaggg
     2581 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2641 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2701 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2761 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2821 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2881 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2941 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3001 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3061 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3121 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3181 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3241 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3301 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3361 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3421 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3481 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3541 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3601 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3661 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3721 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3781 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3841 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3901 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3961 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4021 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4081 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4141 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4201 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4261 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4321 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4381 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4441 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4501 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4561 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4621 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4681 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4741 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
     4801 gaattcgagc tcggtacccg gggatccccc gggctgcagg aattcgatat cgtccattcc
     4861 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     4921 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     4981 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc
     5041 gataattctc cataatagtt ctgttattta taatctccag cactaataaa tgctatacgt
     5101 atatttgtac acaatataat ttcagaattt atattgctac catgactgtc tgagaattgg
     5161 gggaataact tgataattgt tgggattcca ttgttcgtaa acgcaataat attaggtata
     5221 tagaagatac taaatgttct ctccgaggat ataggaatgc tcacaatgga atcgatatat
     5281 ttctacataa tagtattgag attattcctc ttttagtttt atataattca ttatcctatt
     5341 acattatcaa tccttgcatt tcagcttcca ttagacttaa tgactgtttc tcaattttta
     5401 tgtcatcttc ctggacctca tgtgatacta taccagtagc atgaatacta ctgaatcgat
     5461 gatactttag agtttcattg caacagtttc aacacagcct ggcatgaaca gtggtaaaag
     5521 tatttcgtcc actattttcg gctactataa ataaatgttt ttttcgcatg catgt
//
