LOCUS       sequence-178960-        7845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains nrp1b specific gene promoter driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;nrp1bP1Egfp
FEATURES             Location/Qualifiers
     source          1..7845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1008..1017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1014..1016,1017..1019,1020..1730)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1014..1016,1017..1019,1020..1730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1059..1080)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1320..1339)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1667..1688
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1731..1796
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(1817..1841)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1867..1887)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1895..1911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1927..2061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(2500..2687,2688..2699)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2733..2749)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2786..2804)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2825..2841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2838..2860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2849..2865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2873..2879,2880..2897,2898..2903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2918..2939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3056..3073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3227..3815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3307..3326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3986..4777,4778..4846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4609..4628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4847..4951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5114..5135)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5484..5495,5496..5658)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    6027..6148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6271..6981)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(6351..6374)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6549..6568)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6818..6836
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      6978..6987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     7629..7645
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7665..7685
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     7687..7706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7687..7705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7714..7821
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     7730..7746
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7731..7747
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7781..7797)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(join(7834..7845,1..7))
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gttaattcaa gtttgtacaa aaaagcaggc tcgctaaatg acatgtgtga tattaactca
       61 acagtgacat gcttgctttc ctgcacctat taatggatta agactgcaca tattttttat
      121 tttattttac tatgtgaatt gggtaagctt aactgacagt agtgtatgtg tgtgaatgag
      181 agtgtatgga tgtttcccaa tgatgggttg cagctggaag aataattatt aatattattt
      241 agatcagagc aaactatttt taactattag aggacatacc ccttccttca cccccatata
      301 tcttgttttg atgtttgcca gggaggatca agcattactt agggggtcag accccccccc
      361 ctccccccat tcacattctg acttagagta aaatatctaa cttgtaatgt tagtgtttac
      421 tgaaacctta tttgaaaatg cagttattaa catatacaac agatgcgtaa acaaacatac
      481 ctcaactttt ttattgactt cttcttaggt tgtcacgtta attgggggtg ccaaacccct
      541 catttctctg cactgcgctc ccacgcagag gagcgcgaag tctctctggt ttggcccccg
      601 gtctccgtgt ctggttggca gagagagaca gcgcgtcggt ctctgcctgg aaggggtgag
      661 agggtttgct cgtttccagt cctgcccatt ttctctcaag gaggaggaac gggagcaacc
      721 agtgctgccg agagttttga caccctcagt cttcatatcg gatgctgaca ccttcgcact
      781 agtgagaacc tgctaaatat acccagcagt gcccgtgtac gcgcgacaca gaggacggaa
      841 atcagtcata tattagggag tgacccgact gctgaagcgc ccgcagacag cgagccgagc
      901 ccaggacgcg ctgtcaagca tctccaacac ttcggattat gaaattgaag caaacacagc
      961 agtggcggtg actctacgag aatctcccgc tttccagggt tgtgatcgcc accatggtga
     1021 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1081 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1141 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1201 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1261 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1321 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1381 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1441 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1501 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1561 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1621 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1681 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     1741 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     1801 gatctagata actgataccc agctttcttg tacaaagtgg ggcgcgccta cgtaactagt
     1861 ctcgagcaac tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc
     1921 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1981 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2041 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2101 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     2161 tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga tcctgtggct
     2221 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     2281 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     2341 agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa
     2401 tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc
     2461 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
     2521 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     2581 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     2641 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
     2701 ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag cggccgccac
     2761 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     2821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2881 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3841 ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg agattatcaa
     3901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     4981 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5041 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5101 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5161 caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa ctcatcagcc
     5221 tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg gctactacat
     5281 ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca atgctcttgg
     5341 atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa tgccctctga
     5401 tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc acacaggtca
     5461 gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt
     5521 gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa
     5581 tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag
     5641 taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa
     5701 cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc
     5761 aaactataag acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga
     5821 tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg
     5881 ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt
     5941 gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg
     6001 tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac aaaccacaac
     6061 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     6241 ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca tgccgccggt
     6301 ggagtggtgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga
     6361 ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
     6421 cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct tcagcctctg
     6481 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
     6541 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
     6601 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
     6661 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
     6721 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
     6781 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt
     6841 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
     6901 cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttgtc
     6961 atcctcgccc ttgctcacca tggtggcgac cggtaatgtc agacctggta actccttact
     7021 gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg aggctaatag
     7081 aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg aggcagaccc
     7141 ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc tggaatggca
     7201 ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc tgacctgaga
     7261 atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat caccggaaaa
     7321 taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga acgtcagtat
     7381 ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt aaatatcaca
     7441 gataatcaat cgcttttttt tttcttgact gaaaagctta gactggaaaa ttctttgaaa
     7501 aagtttgcac tacagaaaat aaatacataa catcctgcac tgaatgcaca ctattaatag
     7561 aaaagagagc attattgtag gaaatgtatt gtatatgtac attctgtgca tttcacaatt
     7621 ttctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa
     7681 agttggtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
     7741 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg
     7801 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagg
//
