LOCUS       sequence-17897-e         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p144 pSV-EGFP/beta-gal
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(254..287)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      296..305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(347..368)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaagcttggg atctctataa
       61 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
      121 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      181 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      241 cggtctggta ccgataactt cgtatagcat acattatacg aagttatcac cggtcgccac
      301 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      361 cggcgacgta aacggccaca agttcaacgt gtccggcgag ggcgagggcg atgccaccta
      421 ccgcaagctg accctgaagt tcatctgcac c
//
