LOCUS       sequence-178975-        8011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains dll4 enhancer 1 sequence, and dll4 specific gene promoter 
            driving expression of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Quillien A, Abdalla M, Yu J, Ou J, Zhu LJ, Lawson ND
  TITLE     Robust Identification of Developmentally Active Endothelial 
            Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.
  JOURNAL   Cell Rep. 2017 Jul 18;20(3):709-720. doi: 
            10.1016/j.celrep.2017.06.070.
  PUBMED    28723572
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-07-18- 18"; volume: "20"; issue: "3"; pages: "709-720"
COMMENT     Sequence Label: pT2-cryR;dll4CE1-P1Egfp
FEATURES             Location/Qualifiers
     source          1..8011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..26
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    680..704
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1360..1369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1366..1368,1369..1371,1372..2082)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1366..1368,1369..1371,1372..2082)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1411..1432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1672..1691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2019..2040
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2083..2148
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(2169..2193)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2219..2239)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2247..2263)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2279..2413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(2852..3039,3040..3051)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3085..3101)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3085..3101)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3138..3158)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3138..3156)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3177..3193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3190..3212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3201..3217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3225..3231,3232..3249,3250..3255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3270..3291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3408..3425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3579..4167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3659..3678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4338..5129,5130..5198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4961..4980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5199..5303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5466..5487)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5836..5847,5848..6010)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    6379..6500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6623..7333)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(6703..6726)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6901..6920)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7170..7188
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7330..7339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     7981..7997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctatcaact ttgtatagaa aagttgcagc tgaacactga aaaggcagag aaaaggctgc
       61 cctcaggaac agccgaccgt ttttcatgat aaggatattt cattgttttt gcaacaagac
      121 agcttgagtt taaaaaaaaa aaaaaaagtc ctacagccga gcggctcagt tgtttggcgg
      181 tgatataatg cccgttcagt ctttgttgtg tgggtgtcag ttggcggttg ttgagagggg
      241 gggcatgagt agtgttgccc ctttgtttgt tttcaatggc tcataaacag tctgcataaa
      301 ccgatagaaa aaggccagct tccccttcgc tctgtgtgcg gctgattcct cgttcacact
      361 cctcgggtcc tacgactctg ggattgtttt gagctgtcca gcgaggaagg aagccagtgt
      421 gagaaagtgg ctgttttttt ggagtggaca ggatttggcc tcatcgcgcg cggttcctct
      481 tttcgtcggc tgaaacaatg cagtggcgtc acacccctcg actggtgctc ggcgtttcac
      541 atggaaaaat gttcccagaa ttccgctccc aggctcagag gtaaggctgg gaatggagag
      601 cggaggaaac gcagagcgag agacgaacag gaatggagga aaagccagag acctgagcag
      661 aactgaacag tgctcatatg caagtttgta caaaaaagca ggctcgctag gatactaaaa
      721 tgcgctttca ggctgtccca gcttgaaaca tgaagaggct ctgctccgga acatcaggta
      781 aggcatgacc ctccactgac cgtgcacgct ttcagtgatg taatataatt atccgcgcaa
      841 ttaactggaa cgaggacaaa cgacaagtaa taggctgtgc gttgtgcgaa gtttcatccg
      901 atttagtttt tcttcagata cgcgtattct gcactgcatg gtgtgaaatg gtcagtattt
      961 aatcgtgcct gctttaaata tgccaacaga tgctgggctt gttaataatc actcgcgctc
     1021 aattggtcat catcatccgc gtagtggtgg gtattaaatg tggatatcat tgttgccaag
     1081 agaggggggc tgactgagca taagtgggcg gcgtttagcg atagacaaga gtttatataa
     1141 tgagcagcgc ggacacaaag ccagtataaa tactgaggga cgtaagaaac aagtcttcat
     1201 tcacgaatta cacgagaagc gcgaggatgc ccaacagcgc aatgctgacg cacaaagttt
     1261 agaagcaaaa caagacataa ccattgacac taaacacatc ggtgcgctca ctgtggaata
     1321 ccaaacatgc tcagtggata tcttgagacg aagaggatcg ccaccatggt gagcaagggc
     1381 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1441 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1501 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1561 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1621 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1681 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1741 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1801 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1861 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1921 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1981 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2041 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga
     2101 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga
     2161 taactgatac ccagctttct tgtacaaagt ggggcgcgcc tacgtaacta gtctcgagca
     2221 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     2281 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2341 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2401 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2461 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     2521 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     2581 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     2641 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     2701 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     2761 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     2821 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     2881 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     2941 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     3001 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     3061 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     3121 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     3181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4201 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     4261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     5341 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5401 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5461 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5521 gaaaaaccgt ctatcagggc gatggcccac gctggctaag aactcatcag cctccccggt
     5581 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     5641 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     5701 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     5761 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
     5821 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     5881 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     5941 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     6001 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
     6061 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
     6121 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
     6181 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
     6241 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
     6301 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
     6361 acaggcccat ttacgcctta agatacattg atgagtttgg acaaaccaca actagaatgc
     6421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6481 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     6541 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     6601 atgatctaga gtcgcggccg ctttacttgt acagctcgtc catgccgccg gtggagtggt
     6661 ggccctcggc gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
     6721 ccagcttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
     6781 tggtcttgac ctcggcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
     6841 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg
     6901 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
     6961 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     7021 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
     7081 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga
     7141 tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc
     7201 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     7261 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgttg tcatcctcgc
     7321 ccttgctcac catggtggcg accggtaatg tcagacctgg taactcctta ctgtaactta
     7381 ttgcacctga tgaacaaaag atgtgaactg aacgcaagta ggaggctaat agaggctagt
     7441 acagtacaaa gatccccaaa tgatgtgtgc cggccttagt ggaggcagac ccctggttat
     7501 atacctgtgc tgcagaaaga ggggcttaca aatggatctc tctggaatgg cattatctca
     7561 tggtgggaac agtgttgtca gcagaatcag cggggtggac tctgacctga gaatgacagt
     7621 ctagcactga atggctcaga caatactctt gtggccaact atcaccggaa aataagtgcc
     7681 tgccaaaaca aaccagacca gatgaagcta catggctgta gaacgtcagt atttaatgtg
     7741 ttgtggccac ttggttcacc agatttaaga gctaattttt ttaaatatca cagataatca
     7801 atcgcttttt tttttcttga ctgaaaagct tagactggaa aattctttga aaaagtttgc
     7861 actacagaaa ataaatacat aacatcctgc actgaatgca cactattaat agaaaagaga
     7921 gcattattgt aggaaatgta ttgtatatgt acattctgtg catttcacaa ttttctcgag
     7981 caggaaacag ctatgaccat gattacgcca a
//
