LOCUS       sequence-178979-        9406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-HA; PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9406)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_402
FEATURES             Location/Qualifiers
     source          1..9406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    67..85
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    103..121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    139..157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    174..192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    210..228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    290..407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(620..664)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    849..1082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1575..1700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1747..1927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1928..2154)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2182..2202)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2182..2200)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2221..2237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2221..2237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2234..2256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2245..2261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2269..2275,2276..2293,2294..2299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2452..2469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2623..3211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2703..2722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3382..4173,4174..4242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4005..4024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4243..4314)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4314..4449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4489..4610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4500..4519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4554..4573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4682..4915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5120..5131
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5529..5549
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5589..5597,5598..5633,5634..5636,5637..5672,
                     5673..5675,5676..5711,5712..5717,5718..6335))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6336..6389)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6390..6986)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6470..6490)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6967..6986
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6996..7506)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7059..7077)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7516..7533)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7549..7575)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7583..7707
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7850..7869)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(8407..9066)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8981..9000
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(9120..9126,9127..9144,9145..9150))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(9175..9299)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(join(9328..9406,1..236))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(9331..9355)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    join(9402..9406,1..14)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atcactgata gggagtaaac tcgacatacg ttctctatca ctgataggga taaactcgac
       61 atacgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg
      121 agtaaactcg acatacgttc tctatcactg atagggagta aactcgacat cgttctctat
      181 cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgaca
      241 tatcgattcg cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa
      301 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
      361 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
      421 ctgccatttg tctcgaggtc gagaattgtc ccctcggggt tgggaggtgg gtctgaaacg
      481 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      541 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      601 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
      661 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
      721 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
      781 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
      841 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
      901 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
      961 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     1021 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     1081 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     1141 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     1201 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     1261 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     1321 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     1381 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     1441 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     1501 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     1561 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     1621 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     1681 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     1741 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     1801 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     1861 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     1921 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
     1981 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
     2041 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
     2101 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
     2161 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     2221 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     2281 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     2341 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2401 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3061 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3661 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aaaggcctcc
     4321 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     4381 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     4441 gggcgggatg ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg
     4501 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4561 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc
     4621 tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta
     4681 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     4741 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     4801 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     4861 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     4921 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     4981 ggtacctcta gtccggacgc cgaggcgaaa caggcgggga ggcggcccaa agggagatcc
     5041 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     5101 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca
     5161 gaatccaggt ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca
     5221 ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg
     5281 cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac
     5341 aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc
     5401 cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca
     5461 aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag
     5521 cgtatccaca tagcgtaaaa ggagcaacat agttaagaat acgatatctt ggatcgatcc
     5581 ttacttagtt acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
     5641 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
     5701 agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
     5761 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
     5821 tgtctcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
     5881 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
     5941 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
     6001 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
     6061 gagaggagag cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
     6121 ccttccaggg ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
     6181 agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
     6241 tgagcgagtt tccttgtcgt caggccttcg ataccgacac cattgagtaa ttccagagct
     6301 ccgtttatga ctttgctctt gtccagtcta gacattggac cagggttttc ttcaacatca
     6361 ccacaagtga ggagagaacc tctaccttcg gcaccgggct tgcgggtcat gcaccaggtg
     6421 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     6481 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     6541 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     6601 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     6661 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     6721 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     6781 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     6841 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     6901 tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg
     6961 cgcaccgtgg gcttgtactc ggtcatggtg aattgctggg gagagaggtc ggtgattcgg
     7021 tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc
     7081 gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc
     7141 cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt
     7201 cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg
     7261 gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc
     7321 ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg
     7381 cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt cccggaacca
     7441 cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc
     7501 aaccccggat ccttagtggt ggtggtggtg gtggaccgga cgcgtttacg cataatccgg
     7561 cacatcatac ggataaccgg taaccacttt gtacaagaaa gctgaacgag aaacgtaaaa
     7621 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     7681 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
     7741 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
     7801 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
     7861 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggagactt
     7921 tatctgacag cagacgtgca ctggacaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
     7981 taatatcact ctgtacatcc acaaacttgt tgatcaatcg atgctagcgg ctctctcttt
     8041 tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg
     8101 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
     8161 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
     8221 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
     8281 gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat
     8341 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     8401 agatctttac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     8461 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     8521 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     8581 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     8641 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     8701 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     8761 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     8821 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     8881 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     8941 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     9001 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     9061 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
     9121 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt
     9181 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     9241 aatatattga tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtt
     9301 gatcaatcga tgctagccaa ttctccaggc gatctgacgg ttcactaaac gagctctgct
     9361 tatataggcc tcccaccgta cacgcctacc tcgacatacg ttctct
//
