LOCUS       sequence-17901-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p133 pSV-STOP-luc
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      99..234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    complement(259..292)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          826..891
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cgataggtcc gagctcttac gcgtgctagc ccgggctcga gatctgcgat ctgcatctca
       61 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
      121 gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg
      181 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagtga
      241 acctcttcga gggacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg
      301 ttccggatcc tcggggacac caaatatggc gatctcggcc ttttcgtttc ttggagctgg
      361 gacatgtttg ccatcgatcc atctaccacc agaacggccg ttagatctgc tgccaccgtt
      421 gtttccaccg aagaaaccac cgttgccgta accaccacga cggttgttgc taaagaagct
      481 gccaccgcca cggccaccgt tgtagccgcc gttgttgtta ttgtagttgc tactgttatt
      541 tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct cgttgttgtc
      601 gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa atgcttttct
      661 tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt tcccgttcct
      721 ccatctcttt tatatttttt ttctcgaggg atctttgtga aggaacctta cttctgtggt
      781 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
      841 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
      901 tatggaactg a
//
