LOCUS       sequence-17902-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p133 pSV-STOP-luc
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    72..105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    130..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(154..173)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     208..227
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(689..709)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccggggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa cagtaccgga
       61 atgccaagct tataacttcg tataatgtat gctatacgaa gttatcctag gagcttactt
      121 accatgtcag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
      181 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      241 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      301 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      361 gattatgatc tctagtcaag gcactataca tcaaatattc cttattaacc cctttacaaa
      421 ttaaaaagct aaaggtacac aatttttgag catagttatt aatagcagac actctatgcc
      481 tgtgtggagt aagaaaaaac agtatgttat gattataact gttatgccta cttataaagg
      541 ttacagaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt
      601 aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttagca
      661 attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat
      721 gttgagagtc agcagtagcc tcatcatcac
//
