LOCUS       sequence-179086-        7465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOBIR1 split YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, 
            Joosten MH, Robatzek S
  TITLE     Avr4 promotes Cf-4 receptor-like protein association with the 
            BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and
            plant immunity.
  JOURNAL   New Phytol. 2016 Apr;210(2):627-42. doi: 10.1111/nph.13802. Epub 
            2016 Jan 14.
  PUBMED    26765243
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.13802";
            journalName: "New Phytol"; date: "2016-04"; volume: "210"; issue: 
            "2"; pages: "627-42"
COMMENT     Sequence Label: p35S::SISOBIR1-YFPc
FEATURES             Location/Qualifiers
     source          1..7465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     347..363
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     348..364
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    380..404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2316..2340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2351..2602
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2539..2560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2894..2918
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      3030..3739
                     /label=oriV
                     /note="incP origin of replication"
     promoter        3812..3903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3904..3972,3973..4764)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4122..4141)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4935..5523
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5424..5443
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5835..5859
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(5953..6205)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6225..6776)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(6818..6997)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ggtcgacggt
      361 atcgataagc ttcccatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      421 caccatgact tcgaatatcc acttttttct tttatacgtt gtgagccttt tcctttttgt
      481 tcaagcaaga ttgaatcttt atccaccaga tcatgctgca ctcttgcttg tacaaaagga
      541 cttaggcatc atttctgtta acaacccgtg tacgctcgca ggaatatcgt gcgagcgtag
      601 accgggtaac acaacacaag ttgtgagagt tacccgtatt gtattcagat ccaacggatt
      661 aaagggaact ttgtcttctg ctattggcaa actcactgag ctcaaagagc tttctctttc
      721 cgacaatcaa ctatctgaac aaatccccgt tcagattatt gattgtcgga agttagagat
      781 tcttcaactt caaaggaaca gattttctgg gaagattcca tccgaattgt cagctttaaa
      841 ccgtttaagg atagttgact tttcatcgaa tgagttttcg gggaatcttg atttcttgaa
      901 gtactttcct aatctggaaa aactgtctct ggctgataat atgttcactg gaaaaatacc
      961 attttcattg aaatctttcc gaaatcttcg ttttctcaac atttcaggga atagtttcct
     1021 tgaaggtcca gtacctgtca tgagccaaat tgagcattta tcagcagatt tgaatcgaaa
     1081 aaatggcgtt cctaaacgtt acattctagc tgagaattca acaaggatat ctgcaatggg
     1141 acctgcttcg gttccagctc cagctccagc acctgtcaat cgtgttgtac cggcgatgca
     1201 taaacgtaag aacaagaaaa ggaagttacg atcttggttt cttggtttcc tagctggaac
     1261 ttttgctggg ggtatatctg ctgtgctctg ttccttgcta ttcaagatgg taatgttctt
     1321 cgtaagaaga gggaataacg attcaagttt aacgatatac agtccactga tcaagaaagc
     1381 cgaggacttg gctttcttag agaaggaaga tggagtggca tcgcttgaaa tgattggaaa
     1441 aggtggctgt ggagaagttt atagagcaga gctaccgggg agtaatggga agattatagc
     1501 tataaagaag attatccaat ccccaatgga tgctgcagag atcacggagg aagatactaa
     1561 ggcgttgaac aagaaaatgc gtcaagttaa atcagaaatt caaattgtag gtcaaatcag
     1621 acaccggaat ctgcttccat tactcgcgca tatgccaagg ccagactgtc attacttggt
     1681 ctacgaatac atgaaaaatg ggagtttaca ggatatcctg cagcaagtca cagaaggcac
     1741 aagggaatta gattggttgg gacgacacag aattgcagcg ggggtagctg ctggtctcga
     1801 gtatctccat ataaaccata ctcaacgcat aattcacaga gatttaaagc cagcaaatat
     1861 cctacttgat gatgacatgg aagctcgagt tgctgatttt ggccttgcaa aggcagttcc
     1921 agatgctcat acacatatca ccacttcaaa tgttgcagga actgttggat ttattgcacc
     1981 agaatattat cagacactga agtttacaga taagtgtgat atatacagct ttggtgtggt
     2041 gctagctgtg ttggttatcg gtaagggtcc atcggatgat tttttccaac atacttctga
     2101 gatgagttta gttaagtggc tgaggaatgt aatgacttct gacgatccta aaatagcaat
     2161 tgatcctaag cttataggaa atggatacga cgaacaaatg cttttggttc tcaagatagc
     2221 ttgcttttgt actctcgaca atccaaagga gaggcctaac agtaaggatg ttaggtgcat
     2281 gttaactcag atcaagcata agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     2341 tgatgggatg gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     2401 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2461 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2521 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2581 cggcatggac gagctgtaca agtaatctag agtccgcaaa aatcaccagt ctctctctac
     2641 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa
     2701 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     2761 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg
     2821 accgggcggc cgccaccgcg gtggaggggg atcagattgt cgtttcccgc cttcagttta
     2881 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2941 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     3001 tacatcaccg acgagcaagg caagaccgag cgcctttccg acgctcaccg ggctggttgc
     3061 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
     3121 gccgtgtgcg agacaccggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca
     3181 ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga
     3241 cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg
     3301 gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa
     3361 gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct
     3421 tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc
     3481 tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct
     3541 aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct
     3601 gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc
     3661 cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc
     3721 ctcaccccaa aaatggcagc gctggcagtc cttgccattg ccgggatcgg ggcagtaacg
     3781 ggatgggcga tcagcccgaa agctacccct atttgtttat ttttctaaat acattcaaat
     3841 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3901 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3961 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4021 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4081 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4141 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4201 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4261 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4321 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4381 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4441 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4501 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4561 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4621 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4681 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4741 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4801 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4861 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4921 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4981 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5041 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5101 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5161 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5221 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5281 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5341 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5401 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5461 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5521 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5581 atggactcta gctagaggat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     5641 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     5701 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     5761 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     5821 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     5881 aataacacat tgcggacgtt tttaatgatc gaatactaac gtctctacca gatatcagct
     5941 tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     6001 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     6061 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     6121 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     6181 tttattgcca aatgtttgaa cgatctgctt gactctaggg gtcatcagat ttcggtgacg
     6241 ggcaggaccg gacggggcgg caccggcagg ctgaagtcca gctgccagaa acccacgtca
     6301 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc
     6361 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg
     6421 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc
     6481 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt
     6541 gccttccagg gacccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc
     6601 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc
     6661 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc
     6721 ggcatgtccg cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatggta
     6781 gatcccctcg atcgagttga gagtgaatat gagactctaa ttggataccg aggggaattt
     6841 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
     6901 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
     6961 aacccgcggc tcagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
     7021 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc
     7081 agattgtcgt ttcccgcctt cggtttgggc gcgcccggtt cagaagacca gagggctatt
     7141 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     7201 tgtcacttca tcgaaaggac agtagaaaag gaagatggct tctacaaatg ccatcattgc
     7261 gataaaggaa aggctatcgt tcaagatgcc tctaccgaca gtggtcccaa agatggaccc
     7321 ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     7381 gattgatgtg atacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     7441 cagtctcaga agaccagagg gctat
//
