LOCUS       sequence-17909-e         453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Floxed RFP inserted within the coding sequence of cre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Kaczmarczyk SJ, Green JE
  TITLE     A single vector containing modified cre recombinase and LOX 
            recombination sequences for inducible tissue-specific amplification 
            of gene expression.
  JOURNAL   Nucleic Acids Res 2001 Jun 15;29(12):E56-6.
  PUBMED    11410679
REFERENCE   2  (bases 1 to 453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2001-06-15"; volume: "29(12)"; pages: "E56-6"
COMMENT     Sequence Label: p224 pCMV-RFP/CREM
FEATURES             Location/Qualifiers
     source          1..453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    129..162
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(363..383)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg ttctccatag
       61 aagacaccga ctctagagga tcccctcgag cggccgccta ggccatggcg aatttactga
      121 cggtaccaat aacttcgtat aatgtatact atacgaagtt atctagaccg gtcgccacca
      181 tggtgcgctc ctccaagaac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg
      241 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      301 gccncnacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      361 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
      421 ccgactacaa gaagctgtcc ttccccgaag gct
//
