LOCUS       sequence-179092-       13134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xHA-tagged 
            constitutively active MKK6 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo 
            Z, Yang D, Luo H, McManus BM, Janes KA
  TITLE     An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis 
            during coxsackievirus B3 infection.
  JOURNAL   Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 
            10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.
  PUBMED    23332156
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2012.11.009"; journalName: "Cell Host Microbe"; 
            date: "2013-01-16- 16"; volume: "13"; issue: "1"; pages: "67-76"
COMMENT     Sequence Label: pSLIK 3xHA-MKK6-EE neo
FEATURES             Location/Qualifiers
     source          1..13134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2613..2637
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2669..2983
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2713..2731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2748..2766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2784..2802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2820..2838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2891..2909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2956..2980
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2995..3012
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3016..3096
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(4143..4167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4217..5428
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5265..5284
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5487..6104,6105..6110,6111..6146,6147..6149,6150..6185,
                     6186..6188,6189..6224,6225..6233)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6271..6844
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6438..6455)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6665..6684
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6860..7654
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6860..7654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6914..6933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7524..7543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7822..8410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7875..7895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8293..8304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8413..8429)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8414..8430)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8939..9119
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9145..9162)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9151..9375
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9421..9849
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9508..9527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9718..9739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9858..9878)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9863..10192
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10043..10178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10105..10124
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10240..10287
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10306..10680
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10810..10931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10847..10866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10901..10920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10980..10996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10980..10996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10993..11015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11004..11020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11028..11034,11035..11052,11053..11058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11073..11094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11211..11228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11382..11970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11462..11481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12141..12932,12933..13001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12764..12783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13002..13106)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattct agacagttat caacaagttt gtacaaaaaa gcaggcttta
     2641 aaggaaccaa ttcagtcgac tggatcccga gtttactccc tatcagtgat agagaacgta
     2701 tgtcgagttt actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga
     2761 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
     2821 ccctatcagt gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg
     2881 tcgagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga
     2941 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gccactagtc ggatctggtt
     3001 ccgcgtggat ctatgtaccc atacgatgtt ccagattacg cttacccata cgatgttcca
     3061 gattacgctt acccatacga tgttccagat tacgctggat ctatgtctca gtcgaaaggc
     3121 aagaagcgaa accctggcct taaaattcca aaagaagcat ttgaacaacc tcagaccagt
     3181 tccacaccac ctagagattt agactccaag gcttgcattt ctattggaaa tcagaacttt
     3241 gaggtgaagg cagatgacct ggagcctata atggaactgg gacgaggtgc gtacggggtg
     3301 gtggagaaga tgcggcacgt gcccagcggg cagatcatgg cagtgaagcg gatccgagcc
     3361 acagtaaata gccaggaaca gaaacggcta ctgatggatt tggatatttc catgaggacg
     3421 gtggactgtc cattcactgt caccttttat ggcgcactgt ttcgggaggg tgatgtgtgg
     3481 atctgcatgg agctcatgga tacatcacta gataaattct acaaacaagt tattgataaa
     3541 ggccagacaa ttccagagga catcttaggg aaaatagcag tttctattgt aaaagcatta
     3601 gaacatttac atagtaagct gtctgtcatt cacagagacg tcaagccttc taatgtactc
     3661 atcaatgctc tcggtcaagt gaagatgtgc gattttggaa tcagtggcta cttggtggac
     3721 gaggttgcta aagaaattga tgcaggttgc aaaccataca tggcccctga aagaataaac
     3781 ccagagctca accagaaggg atacagtgtg aagtctgaca tttggagtct gggcatcacg
     3841 atgattgagt tggccatcct tcgatttccc tatgattcat ggggaactcc atttcagcag
     3901 ctcaaacagg tggtagagga gccatcgcca caactcccag cagacaagtt ctctgcagag
     3961 tttgttgact ttacctcaca gtgcttaaag aagaattcca aagaacggcc tacataccca
     4021 gagctaatgc aacatccatt tttcacccta catgaatcca aaggaacaga tgtggcatct
     4081 tttgtaaaac tgattcttgg agactaacaa ttggaagact aatgcgtttt cgagatatct
     4141 agacccagct ttcttgtaca aagtggttga taacatctcg agccataaag atggttaatt
     4201 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     4261 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     4321 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     4381 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4441 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4501 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4561 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     4621 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     4681 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     4741 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     4801 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     4861 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     4921 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     4981 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     5041 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     5101 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     5161 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     5221 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     5281 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     5341 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     5401 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     5461 gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     5521 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5581 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     5641 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     5701 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     5761 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     5821 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     5881 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     5941 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     6001 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     6061 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     6121 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     6181 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     6241 aggatccgcg gccgcactag aggaattccg cccctctccc cccccccccc ctaacgttac
     6301 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6361 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6421 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6481 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6541 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6601 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6661 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6721 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6781 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6841 ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6901 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6961 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7021 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7081 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7141 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7201 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7261 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7321 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7381 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7441 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7501 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7561 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7621 cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct
     7681 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     7741 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     7801 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     7861 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7921 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7981 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8041 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8101 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8161 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8221 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8281 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8341 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8401 gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac
     8461 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     8521 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     8581 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8641 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     8701 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     8761 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     8821 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     8881 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     9181 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     9241 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     9301 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     9361 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     9421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     9481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     9541 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9601 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     9661 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     9721 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     9781 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     9841 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     9901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    10021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
    10141 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
    10201 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
    10261 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
    10321 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
    10381 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
    10441 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
    10501 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
    10561 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
    10621 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
    10681 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10741 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10801 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10861 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10921 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    10981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    11041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    11101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    11161 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    11221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    11281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    11341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    11401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    11461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    11521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    11941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    12001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    12061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    12121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    12181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    12241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    12301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    12361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    12421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    12481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    12721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    12961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    13021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    13081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
