LOCUS       sequence-179095-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab
            to include an additional T residue shortly after the TEV cleavage 
            site. Additional mass used as marker in mass spectrometry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Reid DJ, Keener JE, Wheeler AP, Zambrano DE, Diesing JM, 
            Reinhardt-Szyba M, Makarov A, Marty MT
  TITLE     Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry.
  JOURNAL   Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 
            10.1021/acs.analchem.7b03569. Epub 2017 Oct 23.
  PUBMED    29048874
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.7b03569"; journalName: "Anal Chem"; date: 
            "2017-11-7- 7"; volume: "89"; issue: "21"; pages: "11189-11192"
COMMENT     Sequence Label: pMSP1D1T1
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtcatc atcatcatca tcatcacgat tatgatattc ctactactga gaatttgtat
       61 tttcagggtt ctaccacctt cagtaaactt cgcgaacaac tgggccccgt gacgcaggaa
      121 ttctgggaca acctggaaaa agaaaccgag ggactgcgtc aggaaatgtc caaagattta
      181 gaagaggtga aggccaaggt tcagccatat ctcgatgact ttcagaaaaa atggcaggaa
      241 gagatggaat tatatcgtca aaaggtggaa ccgctgcgtg cggaactgca agagggggca
      301 cgccaaaaac tccatgagct ccaagagaag ctcagcccat taggcgaaga aatgcgcgat
      361 cgcgcccgtg cacatgttga tgcactccgg actcatttgg cgccgtattc ggatgaactt
      421 cgccagcgtt tggccgcacg tctcgaggcg ctgaaagaaa acgggggtgc ccgcttggct
      481 gagtaccacg cgaaagcgac agaacacctg agcaccttga gcgaaaaagc gaaaccggcg
      541 ctggaagatc tacgccaggg cttattgcct gttcttgaga gctttaaagt cagttttctg
      601 tcagctctgg aagaatatac taaaaagctg aatacccag
//
