LOCUS       sequence-179096-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab
            to include two additional T residues shortly after the TEV cleavage 
            site. Additional mass used as marker in mass spectrometry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Reid DJ, Keener JE, Wheeler AP, Zambrano DE, Diesing JM, 
            Reinhardt-Szyba M, Makarov A, Marty MT
  TITLE     Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry.
  JOURNAL   Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 
            10.1021/acs.analchem.7b03569. Epub 2017 Oct 23.
  PUBMED    29048874
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.7b03569"; journalName: "Anal Chem"; date: 
            "2017-11-7- 7"; volume: "89"; issue: "21"; pages: "11189-11192"
COMMENT     Sequence Label: pMSP1D1T2
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtcatc atcatcatca tcatcacgat tatgatattc ctactactga gaatttgtat
       61 tttcagggtt ctaccaccac cttcagtaaa cttcgcgaac aactgggccc cgtgacgcag
      121 gaattctggg acaacctgga aaaagaaacc gagggactgc gtcaggaaat gtccaaagat
      181 ttagaagagg tgaaggccaa ggttcagcca tatctcgatg actttcagaa aaaatggcag
      241 gaagagatgg aattatatcg tcaaaaggtg gaaccgctgc gtgcggaact gcaagagggg
      301 gcacgccaaa aactccatga gctccaagag aagctcagcc cattaggcga agaaatgcgc
      361 gatcgcgccc gtgcacatgt tgatgcactc cggactcatt tggcgccgta ttcggatgaa
      421 cttcgccagc gtttggccgc acgtctcgag gcgctgaaag aaaacggggg tgcccgcttg
      481 gctgagtacc acgcgaaagc gacagaacac ctgagcacct tgagcgaaaa agcgaaaccg
      541 gcgctggaag atctacgcca gggcttattg cctgttcttg agagctttaa agtcagtttt
      601 ctgtcagctc tggaagaata tactaaaaag ctgaataccc ag
//
