LOCUS       sequence-179133-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Lubertozzi D, Keasling JD
  TITLE     Marker and promoter effects on heterologous expression in 
            Aspergillus nidulans.
  JOURNAL   Appl Microbiol Biotechnol. 2006 Oct . 72(5):1014-23.
  PUBMED    16699756
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2006 Oct . 72(5):1014-23."
COMMENT     Sequence Label: pDL12
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1..116
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             128..3175
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(130..152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3351..3918
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(3996..4016)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3996..4014)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4035..4051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4035..4051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4048..4070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4059..4075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4083..4089,4090..4107,4108..4113))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4128..4149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4265..4282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4436..5024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4516..4535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5195..5986,5987..6055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5818..5837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6056..6160)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6228..6246
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6284..6306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6406..6425
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtaagtactt tgctacatcc atactccatc cttcccatcc cttattcctt tgaacctttc
       61 agttcgagct ttcccacttc atcgcagctt gactaacagc taccccgctt gagcagacat
      121 caccatggtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      181 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      241 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
      301 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
      361 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc
      421 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
      481 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
      541 taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg
      601 tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
      661 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
      721 cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca
      781 tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat
      841 gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca
      901 ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
      961 cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc
     1021 gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
     1081 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
     1141 caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt
     1201 catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
     1261 cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
     1321 cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct
     1381 gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
     1441 cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
     1501 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta
     1561 tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
     1621 ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
     1681 acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct
     1741 tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
     1801 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
     1861 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
     1921 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt
     1981 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
     2041 tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
     2101 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
     2161 gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
     2221 cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct
     2281 cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
     2341 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc
     2401 acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg
     2461 tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg
     2521 ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac
     2581 ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg
     2641 gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
     2701 taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg
     2761 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
     2821 tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
     2881 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
     2941 tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca
     3001 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
     3061 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     3121 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     3181 accgggcagg ggggatcgat ccacttaacg ttactgaaat catcaaacag cttgacgaat
     3241 ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat ggtgttcagg
     3301 atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag tgatttaata
     3361 gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat acagctcatc
     3421 tgcaatgcat taatgcattg gacctcgcaa ccctagtacg cccttcaggc tccggcgaag
     3481 cagaagaata gcttagcaga gtctattttc attttcggga gacgagatca agcagatcaa
     3541 cggtcgtcaa gagacctacg agactgagga atccgctctt ggctccacgc gactatatat
     3601 ttgtctctaa ttgtactttg acatgctcct cttctttact ctgatagctt gactatgaaa
     3661 attccgtcac cagcccctgg gttcgcaaag ataattgcac tgtttcttcc ttgaactctc
     3721 aagcctacag gacacacatt catcgtaggt ataaacctcg aaaatcattc ctactaagat
     3781 gggtatacaa tagtaactat ggttgcctag tgaatgctcc gtaacaccca atacgccggc
     3841 cgaaactttt ttacaactct cctatgagtc gtttacccag aatgcacagg tacacttgtt
     3901 tagaggtaat ccttctttct agagtcgacc tgcaggcatg caagcttaag ggcgaattcg
     3961 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     4021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcg
     4261 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4321 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4441 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4921 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4981 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5041 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5101 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5161 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5221 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5281 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5341 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5401 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5461 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5521 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5581 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5641 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5701 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5761 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5821 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5881 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5941 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6001 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6061 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6121 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6181 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     6241 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6301 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6361 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6421 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     6481 accgcatcag gcgatatcta gatctcgagc tcgcgaaagc ttgcatgcaa taattgcagc
     6541 aaatattgat gaagcgagag gtaggacgat gaaggactgt gagcagttca aggtatcagc
     6601 agagtcaagg gcctgatgca atggcggtga tccgtgatca gcgaacggaa ggggcgctaa
     6661 ctctgtttct ttaccaatga tcggaagctc ctgctggcgg acttatgagt cattcacgaa
     6721 tcatttctca gttatttgtg gatgccctcg ttctgtccac aatttctttc cgccccaagt
     6781 cttttaagtt ctttaacatc tatattcttg cacttccaat ggcatccctt cgctccgtac
     6841 tcaagagcca gagcttgcga cacaccgtgc gatcctactc ctcgcaaacc atgcctcccg
     6901 cctcaccctt tgctccccgc cacttcctct ccattgcgga cctctcgccc tccgagttcg
     6961 caacccttgt tcgcaatgcc tcctcacaca aacgggctat caagtcgggg tcaatgcccc
     7021 aaaacttgca gggatcactc cttgggaaaa ctgtggccat gatcttcagc aaacgaagca
     7081 cgaggacaag ggtatctaca gaaggggccg ttgtgcagat gggaggtcat ccgatgttct
     7141 tgggcaagga tgatatccaa ctaggtgtca acgagtccct atacgacacc tccgttgtca
     7201 tttcgtctat ggtatcctgc attgtagccc gtgtcggtaa acatgcagag gtcgcagatc
     7261 tggcgaagca ctcttcggtt ccagtcatca atgctttgtg tgactctttc caccctctcc
     7321 aagccgtggc cgatttccag accatctatg aagcattcac ccccaaggcg caccaccttt
     7381 caagtctagg gttggaagga ttgaagatcg cttgggtggg tgacgccaac aacgtcctgt
     7441 tcgatatggc cattgctgct acaaaaatgg gtgtcgacat tgctgtcgct actcccaagg
     7501 ggtacgaaat ccctcctcac atgctggagc tcatcaagtc tgctggagag ggtgtctcga
     7561 aaccaggaaa gcttctgcaa accaatattc ccgaagaagc ggtcaaggac gccgatattc
     7621 tggtcacaga cacctgggtc tctatgggcc aagaggaaga gaaggctcag aggctgaagg
     7681 agtttgatgg tttccaaatc actgctgaac tcgccaagcg aggaggagct aaggagggct
     7741 ggaagttcat gcactgtctc ccgcgacacc ctgaggaggt cagcgacgag gttttctaca
     7801 gcaaccggtc acttgtcttc cctgaggctg agaaccggtt atgggctgcg atttccgcct
     7861 tggagggttt cgttgtcaat aagggaaaaa ttgaataaat ataaccaggc ttccatttaa
     7921 atatatagag gctggcgtta tcaaactgat gagttgacgg gtatgagatc attccgtccc
     7981 taaatatatt tactccgatc acgtaaaagc ctgttagtag aagcattttc ccaattatcc
     8041 tgaccaattc tttctagcat atatcaaata actaattgac atgttctctc gcttccttat
     8101 attactcaga gtattggaaa tggggcaaat cgcacccggt gactttcaca tgtcacgaat
     8161 gcggagtcgt cctagccaag gtagatccag gcctaacaca ccccaaccct cgactctcct
     8221 cattccatac tcttgacctc tatccagcac attcttctga ggtttcgcaa tggctgtagg
     8281 tcgacggatc ccccgggctg caggtcgact ctagaggatc ctctagagtc gacctgcagc
     8341 ccgggggatc atccgatagc tctgcaaagg gcgttgcaca atgcaagtca aacgttgcta
     8401 gcagttccag gtggaatgtt atgatgagca ttgtattaaa tcaggagata tagcatgatc
     8461 tctagttagc tcaccacaaa agtcagacgg cgtaaccaaa agtcacacaa cacaagctgt
     8521 aaggatttcg gcacggctac ggaagacgga gaagcccacc ttcagtggac tcgagtacca
     8581 tttaattcta tttgtgtttg atcgagacct aatacagccc ctacaacgac catcaaagtc
     8641 gtatagctac cagtgaggaa gtggactcaa atcgacttca gcaacatctc ctggataaac
     8701 tttaagccta aactatacag aataagatgg tggagagctt ataccgagct cccaaatctg
     8761 tccagatcat ggttgaccgg tgcctggatc ttcctataga atcatcctta ttcgttgacc
     8821 tagctgattc tggagtgacc cagagggtca tgacttgagc ctaaaatccg ccgcctccac
     8881 catttgtaga aaaatgtgac gaactcgtga gctctgtaca gtgaccggtg actctttctg
     8941 gcatgcggag agacggacgg acgcagagag aagggctgag taataagcgc cactgcgcca
     9001 gacagctctg gcggctctga ggtgcagtgg atgattatta atccgggacc ggccgcccct
     9061 ccgccccgaa gtggaaaggc tggtgtgccc ctcgttgacc aagaatctat tgcatcatcg
     9121 gagaatatgg agcttcatcg aatcaccggc agtaagcgaa ggagaatgtg aagccagggg
     9181 tgtatagccg tcggcgaaat agcatgccat taacctaggt acagaagtcc aattgcttcc
     9241 gatctggtaa aagattcacg agatagtacc ttctccgaag taggtagagc gagtacccgg
     9301 cgcgtaagct ccctaattgg cccatccggc atctgtaggg cgtccaaata tcgtgcctct
     9361 cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag gagctggcca gcggcgcaga
     9421 ccgggaacac aagctggcag tcgacccatc cggtgctctg cactcgacct gctgaggtcc
     9481 ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc ggtgtgtcgg cggggttgac
     9541 aaggtcgttg cgtcagtcca acatttgttg ccatattttc ctgctctccc caccagctgc
     9601 tcttttcttt tctctttctt ttcccatctt cagtatattc atcttcccat ccaagaacct
     9661 ttatttcccc taa
//
