LOCUS       sequence-179153-       14487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of recombinant proteins in plants. The plasmid 
            contains gfp, which is replaced by the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Mardanova ES, Blokhina EA, Tsybalova LM, Peyret H, Lomonossoff GP, 
            Ravin NV
  TITLE     Efficient Transient Expression of Recombinant Proteins in Plants by 
            the Novel pEff Vector Based on the Genome of Potato Virus X.
  JOURNAL   Front Plant Sci. 2017 Feb 28;8:247. doi: 10.3389/fpls.2017.00247. 
            eCollection 2017.
  PUBMED    28293244
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-02-28"; volume: "8:247. doi"; pages: " 
            10.3389/fpls.2017.00247. eCollection 2017"
COMMENT     Sequence Label: pEff
FEATURES             Location/Qualifiers
     source          1..14487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..428
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1113..1365
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        1527..1706
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1559..1742
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1763..2557
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1817..1836)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2427..2446
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2943..3195
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3292..3316
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      3685..4316
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(4406..5554)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(5853..6647)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(7237..7254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7488..7507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    7939..7963
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8102..8121
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8246..8268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8260..8277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8261..8277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8380..8725
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      9431..9440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(13304..13325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13565..13584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13962..13983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      14229..14481
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ctagggtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
       61 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      121 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      181 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
      241 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
      301 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      361 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      421 agagaacacg ggggactcta gaggatccga aatgcgggtg atagtatcac catacgaagc
      481 tgaagacatt ttaaaaagat caacagacat gctacgaaac attgatagcg gtgttttgtc
      541 tacaaaggaa tgcattaagg ctttcagcac catcactaga gacctgcact gtgcaaaggc
      601 atcttaccaa tggggagttg acacaggttt gtatcaacgt aattgcgcag aaaagcacct
      661 tattgataca gttgaaagta acataaggtt ggcacagccg cttgttaggg agaaggtagc
      721 agtgcatttt tgcaaggatg agcctaaaga acttgttgcc ttcataacta gaaaatatgt
      781 cgaattgact ggagtaggtg ttagggaagc tgttaaaagg gagatgcgga gtctcactaa
      841 gacagttctc aataagatgt ccctcgaaat ggcattctat atgtcaccta gagcttggaa
      901 gaacgcagaa tggctggaat tgaaatttag tcccgtaaag attttcagag atttgcttct
      961 cgatgttgaa actctcaatg aactctgcgc agaggatgac gttcatgtcg ataaagttaa
     1021 tgaaaacggt gatgagaacc atgatctcga acttcaggat gagtgctgaa tttaaattgt
     1081 acaagtaact gcaggtcgac tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     1141 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     1201 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     1261 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     1321 actaggataa attatcgcgc gcggtgtcat ctatgttact agatctacgt accccgcgcc
     1381 agcttggcgt aatcatggtc atagctgttg cgatcgctct gcagataacg cgtggccggc
     1441 ctacagtatg agcggagaat taagggagtc acgttatgac ccccgccgat gacgcgggac
     1501 aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac tcagccgcgg
     1561 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag
     1621 tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc actgacgttc
     1681 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc aaataatctg
     1741 caccggatct ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     1801 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     1861 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     1921 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     1981 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     2041 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     2101 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     2161 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     2221 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     2281 gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt
     2341 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     2401 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     2461 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     2521 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     2581 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     2641 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     2701 gatctcatgc tggagttctt cgcccacggg atctctgcgg aacaggcggt cgaaggtgcc
     2761 gatatcatta cgacagcaac ggccgacaag cacaacgcca cgatcctgag cgacaatatg
     2821 atcgcggcgt ccacatcaac ggcgtcggcg gcgactgccc aggcaagacc gagatgcacc
     2881 gcgatatctt gctgcgttcg gatattttcg tggagttccc gccacagacc cggatgatcc
     2941 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3001 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3061 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3121 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3181 ctatgttact agatcgggac tgtaggccgg ccctcactgg tgaaaagaaa aaccacccca
     3241 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     3301 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     3361 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     3421 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     3481 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     3541 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
     3601 atttctcgct taaccgtgac agagtagaca ggctgtctcg cggccgaggg gcgcagcccc
     3661 tgggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
     3721 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
     3781 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
     3841 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
     3901 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
     3961 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
     4021 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
     4081 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
     4141 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
     4201 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
     4261 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa
     4321 aggcgctcgg tcttgccttg ctcgtcggtg atgtacacta gtcgctggct gctgaacccc
     4381 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     4441 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     4501 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     4561 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     4621 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     4681 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     4741 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     4801 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     4861 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     4921 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     4981 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     5041 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     5101 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     5161 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     5221 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     5281 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     5341 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     5401 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     5461 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     5521 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     5581 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     5641 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     5701 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
     5761 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
     5821 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
     5881 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5941 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     6001 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     6061 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     6121 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     6181 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     6241 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     6301 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     6361 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     6421 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     6481 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     6541 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     6601 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     6661 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     6721 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     6781 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
     6841 tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt
     6901 ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa
     6961 gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt
     7021 tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg
     7081 gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc
     7141 acttaaaaaa ctcaggccgc agtcggtaac tatgcggtgt gaaataccgc acagatgcgt
     7201 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7741 cttatcgcca ctggcagcag gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
     7801 gcgcggaaat ggacgggccc ccggcgccag atctggggaa ccctgtggtt ggcatgcaca
     7861 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
     7921 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttgtg
     7981 aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     8041 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     8101 aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg ggaagggcga
     8161 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8221 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     8281 ttgttaatta ataggcctcg aggtcaacat ggtggagcac gacacgcttg tctactccaa
     8341 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     8401 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     8461 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     8521 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     8581 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     8641 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     8701 aagttcattt catttggaga ggagaaaact aaaccataca ccaccaacac aaccaaaccc
     8761 accacgccca attgttacac acccgcttga aaaagaaagt ttaacaaatg gccaaggtgc
     8821 gcgaggttta ccaatctttt acagactcca ccacaaaaac tctcatccaa gatgaggctt
     8881 atagaaacat tcgccccatc atggaaaaac acaaactagc taacccttac gctcaaacgg
     8941 ttgaagcggc taatgatcta gaggggttcg gcatagccac caatccctat agcattgaat
     9001 tgcatacaca tgcagccgct aagaccatag agaataaact tctagaggtg cttggttcca
     9061 tcctaccaca agaacctgtt acatttatgt ttcttaaacc cagaaagcta aactacatga
     9121 gaagaaaccc gcggatcaag gacattttcc aaaatgttgc cattgaacca agagacgtag
     9181 ccaggtaccc caaggaaaca ataattgaca aactcacaga gatcacaacg gaaacagcat
     9241 acattagtga cactctgcac ttcttggatc cgagctacat agtggagaca ttccaaaact
     9301 gcccaaaatt gcaaacattg tatgcgacct tagttctccc cgttgaggca gcctttaaaa
     9361 tggaaagcac tcacccgaac atatacagcc tcaaatactt cggagatggt ttccagtata
     9421 taccaggcaa ccatggtggc ggggcatacc atcatgaatt cgctcatcta caatggctca
     9481 aagtgggaaa gatcaagtgg agggacccca aggatagctt tctcggacat ctcaattaca
     9541 cgactgagca ggttgagatg cacacagtga cagtacagtt gcaggaatcg ttcgcggcaa
     9601 accacttgta ctgcatcagg agaggagact tgctcacacc ggaggtgcgc actttcggcc
     9661 aacctgacag gtacgtgatt ccaccacaga tcttcctccc aaaagttcac aactgcaaga
     9721 agccgattct caagaaaact atgatgcagc tcttcttgta tgttaggaca gtcaaggtcg
     9781 caaaaaattg tgacattttt gccaaagtca gacaattaat taaatcatct gacttggaca
     9841 aatactctgc tgtggaactg gtttacttag taagctacat ggagttcctt gccgatttac
     9901 aagctaccac ctgcttctca gacacacttt ctggtggctt gctaacaaag acccttgcac
     9961 cggtgagggc ttggatacaa gagaaaaaga tgcagctgtt tggtcttgag gactacgcga
    10021 agttagtcaa agcagttgat ttccacccgg tggatttttc tttcaaagtg gaaacttggg
    10081 acttcagatt ccaccccttg caagcgtgga aagccttccg accaagggaa gtgtcggatg
    10141 tagaggaaat ggaaagtttg ttctcagatg gggacctgct tgattgcttc acaagaatgc
    10201 cagcttatgc ggtaaacgca gaggaagatt tagctgcaat caggaaaacg cccgagatgg
    10261 atgtcggtca agaagttaaa gagcctgcag gagacagaaa tcaatactca aaccctgcag
    10321 aaactttcct caacaagctc cacaggaaac acagtaggga ggtgaaacac caggccgcaa
    10381 agaaagctaa acgcctagct gaaatccagg agtcaatgag agctgaaggt gatgccgaac
    10441 caaatgaaat aagcgggacg atgggggcaa tacccagcaa cgccgaactt cctggcacga
    10501 atgatgccag acaagaactc acactcccaa ccactaaacc tgtccctgca aggtgggaag
    10561 atgcttcatt cacagattct agtgtggaag aggagcaggt taaactcctt ggaaaagaaa
    10621 ccgttgaaac agcgacgcaa caagtcatcg aaggacttcc ttggaaacac tggattcctc
    10681 aattaaatgc tgttggattc aaggcgctgg aaattcagag ggataggagt ggaacaatga
    10741 tcatgcccat cacagaaatg gtctccgggc tggaaaaaga ggacttccct gaaggaactc
    10801 caaaagagtt ggcacgagaa ttgttcgcta tgaacagaag ccctgccacc atccctttgg
    10861 acctgcttag agccagagac tacggcagtg atgtaaagaa caagagaatt ggtgccatca
    10921 caaagacaca ggcaacgagt tggggcgaat acttgacagg aaagatagaa agcttaactg
    10981 agaggaaagt tgcgacttgt gtcattcatg gagctggagg ttctggaaaa agtcatgcca
    11041 tccagaaggc attgagagaa attggcaagg gctcggacat cactgtagtc ctgccgacca
    11101 atgaactgcg gctagattgg agtaagaaag tgcctaacac tgagccctat atgttcaaga
    11161 cctctgaaaa ggcgttaatt gggggaacag gcagcatagt catctttgac gattactcaa
    11221 aacttcctcc cggttacata gaagccttag tctgtttcta ctctaaaatc aagctaatca
    11281 ttctaacagg agatagcaga caaagcgtct accatgaaac tgctgaggac gcctccatca
    11341 ggcatttggg accagcaaca gagtacttct caaaatactg ccgatactat ctcaatgcca
    11401 cacaccgcaa caagaaagat cttgcgaaca tgcttggtgt ctacagtgag agaacgggag
    11461 tcaccgaaat cagcatgagc gccgagttct tagaaggaat cccaactttg gtaccctcgg
    11521 atgagaagag aaagctgtac atgggcaccg ggaggaatga cacgttcaca tacgctggat
    11581 gccaggggct aactaagccg aaggtacaaa tagtgttgga ccacaacacc caagtgtgta
    11641 gcgcgaatgt gatgtacacg gcactttcta gagccaccga taggattcac ttcgtgaaca
    11701 caagtgcaaa ttcctctgcc ttctgggaaa agttggacag caccccttac ctcaagactt
    11761 tcctatcagt ggtgagagaa caagcactca gggagtacga gccggcagag gcagagccaa
    11821 ttcaagagcc tgagccccag acacacatgt gtgtcgagaa tgaggagtcc gtgctagaag
    11881 agtacaaaga ggaactcttg gaaaagtttg acagagagat ccactctgaa tcccatggtc
    11941 attcaaactg tgtccaaact gaagacacaa ccattcagtt gttttcgcat caacaagcaa
    12001 aagatgagac cctcctctgg gcgactatag atgcgcggct caagaccagc aatcaagaaa
    12061 caaacttccg agaattcctg agcaagaagg acattgggga cgttctgttt ttaaactacc
    12121 aaaaagctat gggtttaccc aaagagcgta ttcctttttc ccaagaggtc tgggaagctt
    12181 gtgcccacga agtacaaagc aagtacctca gcaagtcaaa gtgcaacttg atcaatggga
    12241 ctgtgagaca gagcccagac ttcgatgaaa ataagattat ggtattcctc aagtcgcagt
    12301 gggtcacaaa ggtggaaaaa ctaggtctac ccaagattaa gccaggtcaa accatagcag
    12361 ccttttacca gcagactgtg atgctttttg gaactatggc taggtacatg cgatggttca
    12421 gacaggcttt ccagccaaaa gaagtcttca taaactgtga gaccacgcca gatgacatgt
    12481 ctgcatgggc cttgaacaac tggaatttca gcagacctag cttggctaat gactacacag
    12541 ctttcgacca gtctcaggat ggagccatgt tgcaatttga ggtgctcaaa gccaaacacc
    12601 actgcatacc agaggaaatc attcaggcat acatagatat taagactaat gcacagattt
    12661 tcctaggcac gttatcaatt atgcgcctga ctggtgaagg tcccactttt gatgcaaaca
    12721 ctgagtgcaa catagcttac acccatacaa agtttgacat cccagccgga actgctcaag
    12781 tttatgcagg agacgactcc gcactggact gtgttccaga agtgaagcat agtttccaca
    12841 ggcttgagga caaattactc ctaaagtcaa agcctgtaat cacgcagcaa aagaagggca
    12901 gttggcctga gttttgtggt tggctgatca caccaaaagg ggtgatgaaa gacccaatta
    12961 agctccatgt tagcttaaaa ttggctgaag ctaagggtga actcaagaaa tgtcaagatt
    13021 cctatgaaat tgatctgagt tatgcctatg accacaagga ctctctgcat gacttgttcg
    13081 atgagaaaca gtgtcaggca cacacactca cttgcagaac actaatcaag tcagggagag
    13141 gcactgtctc actttcccgc ctcagaaact ttctttaacc gttaagttac cttagagatt
    13201 tgaataagtc tagattttta tttttaattt tctttcaaat acttccatca ggcgcgccat
    13261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
    13321 cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
    13381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
    13441 cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
    13501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
    13561 caaggacgac ggcaactaca agacccgcgc cgaggtatgt agtgtttttg gataactgag
    13621 tttgcctatg attttgtatt tactgagatg tttgtcctct ttctgcaggt gaaccgcatc
    13681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    13741 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    13801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    13861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    13921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    13981 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaacc cgggtgtaca
    14041 agtaatctag cgataccgtc gaggtcgtat cactggaaca acaaccgctg aggctgttgt
    14101 cactctacca ccaccataac tacgtctaca taaccgacgc ctaccccagt ttcatagtat
    14161 tttctggttt gattgtatga ataatataaa taaaaaaaaa aaaaaaaaaa aaaaactagt
    14221 ggtaccgcga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
    14281 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
    14341 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
    14401 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
    14461 tgtcatctat gttactagat cgtatac
//
