LOCUS       sequence-17929-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Ichida M, Finkel T
  TITLE     Ras regulates NFAT3 activity in cardiac myocytes.
  JOURNAL   J Biol Chem. 2001 Feb 2. 276(5):3524-30.
  PUBMED    11044444
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Feb 2. 276(5):3524-30."
COMMENT     Sequence Label: NFAT luciferase reporter
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..182
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    183..212
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    213..242
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     polyA_signal    281..402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(292..311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     346..365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(729..750)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatttt accaacagta ccggaatgcc
       61 aagcttgcgg gtcgctgcag ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat
      121 gcgaagtgga cctgggaccg cgccagatcc ccggaggaaa aactgtttca tacagaaggc
      181 gtggaggaaa aactgtttca tacagaaggc gtggaggaaa aactgtttca tacagaaggc
      241 gtggtacctc ccgggttatg ttagctcagt tacagtacca taagatacat tgatgagttt
      301 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      361 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      421 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      481 cacgtacctt aatattactt acttatcatg gtagcttggg ctggcgtaat agcgaagagg
      541 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa
      601 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
      661 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      721 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
      781 ggcgaaaaac cgtctatca
//
