LOCUS       sequence-17940-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Nemoto S, Finkel T
  TITLE     Redox regulation of forkhead proteins through a p66shc-dependent 
            signaling pathway.
  JOURNAL   Science. 2002 Mar 29. 295(5564):2450-2.
  PUBMED    11884717
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Mar 29. 295(5564):2450-2."
COMMENT     Sequence Label: pcDNA3.1his p66shc
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..172
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(176..184,185..199)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttaccatgg ggggttctca
      121 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      181 gtacgacgat gacgataagg taccgagctc ggatccacta gtccagtgtg gtggaattcg
      241 gcttatgaat ctcctgcccc ccaagcccaa gtacaatcca ctccggaatg agtctctgtc
      301 atcgctggag gaaggggctt ctgggtccac ccccccggag gagctgcctt ccccatcagc
      361 ttcatccctg gggcccatcc tgcctcctct gcctggggac gatagtccca ctaccctgtg
      421 ccccttcttc ccccggatga gcaacctgag gctggccaac ccggctgggg ggcgcccagg
      481 gtctaagggg gagccaggaa gggcagctga tgatggggag gggatcgtag gggcagccat
      541 gccagactca ggccccctac ccctcctcca ggacatgaac aagctgagtg gaggcggcgg
      601 gcgcaggact cgggtggaag ggggccagct tgggggcgag gagtggaccc gccacgggag
      661 ctttgtcaat aagcccacgc ggggctggct gcatcccaac gacaaagtca tgggacccgg
      721 ggtttcctac ttggttcggt acatgggttg tgtggaggtc ctccagtcaa tgcgtgccct
      781 ggacttcaac acccggactc aggtcaccag ggaggccatc agtctggtgt gtgaggctgt
      841 gccgggtgct aagggggcga caaggaggag aaagccctgt agccgcccgc tcagctctat
      901 cct
//
