LOCUS       sequence-17941-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Nemoto S, Finkel T
  TITLE     Redox regulation of forkhead proteins through a p66shc-dependent 
            signaling pathway.
  JOURNAL   Science. 2002 Mar 29. 295(5564):2450-2.
  PUBMED    11884717
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Mar 29. 295(5564):2450-2."
COMMENT     Sequence Label: pcDNA3.1his p66shc
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(68..76,77..91)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
       61 gggtcgggat ctgtacgacg atgacgataa ggtaccgagc tcggatccac tagtccagtg
      121 tggtggaatt cggcttatga atctcctgcc ccccaagccc aagtacaatc cactccggaa
      181 tgagtctctg tcatcgctgg aggaaggggc ttctgggtcc acccccccgg aggagctgcc
      241 ttccccatca gcttcatccc tggggcccat cctgcctcct ctgcctgggg acgatagtcc
      301 cactaccctg tgccccttct tcccccggat gagcaacctg aggctggcca acccggctgg
      361 ggggcgccca gggtctaagg gggagccagg aagggcagct gatgatgggg aggggatcgt
      421 aggggcagcc atgccagact caggccccct acccctcctc caggacatga acaagctgag
      481 tggaggcggc gggcgcagga ctcgggtgga agggggccag cttgggggcg aggagtggac
      541 ccgccacggg agctttgtca ataagcccac gcggggctgg ctgcatccca acgacaaagt
      601 catgggaccc ggggtttcct acttggttcg gtacatgggt tggtgtggag gtcctccagt
      661 caatgcgtgc cctggacttc acacccggac tcaggtcacc agggaggcat cagtctggtg
      721 tgtgaggctg tg
//
