LOCUS       sequence-17944-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Nemoto S, Finkel T
  TITLE     Redox regulation of forkhead proteins through a p66shc-dependent 
            signaling pathway.
  JOURNAL   Science. 2002 Mar 29. 295(5564):2450-2.
  PUBMED    11884717
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Mar 29. 295(5564):2450-2."
COMMENT     Sequence Label: pcDNA3.1his p66shc S36A
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..77
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(81..89,90..104)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cttagcttac catggggggt tctcatcatc atcatcatca tggtatggct agcatgactg
       61 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggtacct aggatccagt
      121 gtggtggaat tcggcttatg aatctcctgc cccccaagcc caagtacaat ccactccgga
      181 atgagtctct gtcatcgctg gaggaagggg cttctgggtc cacccccccg gaggagctgc
      241 ctgccccacc agcttcatcc ctggggccca tcctgcctcc tctgcctggg gacgatagtc
      301 ccactaccct gtgctccttc ttcccccgga tgagcaacct gaggctggcc aacccggctg
      361 gggggcgccc agggtctaag ggggagccag gaagggcagc tgatgatggg gaggggatcg
      421 taggggcagc catgccagac tcaggccccc tacccctcct ccaggacatg aacaagctga
      481 gtggaggcgg cgggcgcagg actcgggtgg aagggggcca gcttgggggc gaggagtgga
      541 cccgccacgg gagctttgtc aataagccca cgcggggctg gctgcatccc aacgacaaag
      601 tcatgggacc cggggtttcc tacttggttc ggtacatggg ttgtgtggag gtcctccagt
      661 caatgcgtgc cctggacttc acaccc
//
