LOCUS       sequence-17972-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     Unpublished, Paco
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYES2.1-TRP1URA3
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             58..729
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 atcggactac tagcagctgt aatacgactc actataggga atattaagct cgcccttatg
       61 tctgttatta atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc
      121 acagaggccg cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg
      181 cccaatagaa agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa
      241 gcatataaaa atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct
      301 aaggaggatg ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga
      361 gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta
      421 tttccaaaag actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt
      481 cccttgtttg attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct
      541 gactgggttg gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg
      601 acgccagaaa atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc
      661 ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat
      721 gctaagaaaa agggcgagct tcgaggtcac ccatcgaaa
//
