LOCUS       sequence-179764-       10381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector featuring FcpB promoter in front of 
            AttR1/2 sites on episomal vector maintained in the diatom 
            Phaeodactylum tricornutum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Bielinski VA, Bolt TM, Dupont CL, Weyman PD
  TITLE     Episomal tools for RNAi in the diatom Phaeodactylum tricornutum
  JOURNAL   PeerJ Preprints. 2017 5:e2907v1
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            Preprints. 2017 5:e2907v1"
COMMENT     Sequence Label: pFcpB-DEST
FEATURES             Location/Qualifiers
     source          1..10381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..23,24..29)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             77..1267
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(429..448)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             1906..2097
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2091..2113)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2328..2699)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2732..2841
                     /note="incP origin of transfer"
     primer_bind     3103..3125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3117..3134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3118..3134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3604..3728
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3753..3758,3759..3776,3777..3783)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3837..4496
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3903..3922)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4838..5143
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5022..5041
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5184..5308)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5560..5578
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             6282..6656
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    6962..7465
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7466..7652
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             7653..8315
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(8374..8391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8545..9133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8625..8644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9304..10095,10096..10164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9927..9946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10165..10269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10337..10355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgacagctt atcatcgata agctttaatg cggtagttta tcacagttaa attgctaacg
       61 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
      121 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
      181 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
      241 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
      301 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
      361 cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      421 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      481 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      541 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      601 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag
      661 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      721 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
      781 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
      841 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
      901 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
      961 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     1021 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     1081 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     1141 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     1201 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     1261 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
     1321 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc
     1381 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca
     1441 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     1501 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     1561 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     1621 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     1681 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     1741 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     1801 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     1861 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     1921 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     1981 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     2041 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2101 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2161 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg gatcgtcttg ccttgctcgt
     2221 cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
     2281 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
     2341 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
     2401 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
     2461 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
     2521 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
     2581 gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
     2641 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     2701 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     2761 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     2821 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     2881 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     2941 ataatgaccc cgaagcaggg ttatgcagcg gaagatgcca ttcgccattc aggctgcgca
     3001 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     3061 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     3121 aaacgacggc cagtgaatct cgcctattca tggtgtatac aagttcaaca tccaaagctg
     3181 gaacttttgg aaacagaaag aatgtccgaa tagggcacgg cgtgccgtat tgttggagtg
     3241 gactagcaga aagtgaggaa ggcacaggat gagtttcctc gagacacata gcttcagcgt
     3301 cgtgtaggct aggcagaggt gagttttctc gagacatacc ttcagcgtcg tcttcactgt
     3361 cacagtcaac tgacagtaat cgttgatccg gagagattca aaattcaatc tgtttggacc
     3421 tggataagac acaagagcga catcctgaca tgaacgccgt aaacagcaaa tcctggttga
     3481 acacgtatcc ttttgggggc ctccagctac gacgctcgcc ccagctgggg cttccttact
     3541 atacacagcg catatttcac ggttgccaga agtcaagtcg acggtatcga taagcttgat
     3601 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     3661 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     3721 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     3781 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     3841 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     3901 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     3961 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     4021 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     4081 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     4141 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     4201 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     4261 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     4321 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     4381 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     4441 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     4501 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     4561 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     4621 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     4681 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     4741 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     4801 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     4861 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     4921 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     4981 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     5041 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     5101 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     5161 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     5221 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     5281 ctcgttcagc tttcttgtac aaagtggtga attctgagct acctcgactt tggctgggac
     5341 actttcagtg aggacaagaa gcttcagaag cgtgctatcg aactcaacca gggacgtgcg
     5401 gcacaaatgg gcatccttgc tctcatggtg cacgaacagt tgggagtctc tatccttcct
     5461 taaaaattta attttcatta gttgcagtca ctccgctttg gtttcacagt caggaataac
     5521 actagctcgt cttcaggacc atgattacgc caagctcgaa attaaccctc actaaaggga
     5581 acaaaagctg gtacctaaca ggattagtgc aattcgagtt gaatcactgg gaaaaacatt
     5641 gtcttctttt ttatattatc atttgcatta gtgctgcagt cgtagatact tgttggttga
     5701 aagacatcag ctgggaggga ctggactagc gtttggtaag gagacatacc tgttaacgtt
     5761 ggttgcaaaa ttccatttcg cgatttatgt tatctgtaaa tcctgatttg tctggaattc
     5821 ttgatacttc cgttttttta gaggccaatg attagcatcg gcgattctca aaatagcatt
     5881 ttcgacatgc ggtgctgatt tcataaacat agacaacgct tttacatgta aaagtaactt
     5941 gcggacttgg aacagtgctc tgtttttggt gtgaacgtaa ctcagcaata tttctgtgct
     6001 agcaaggttt tttatgatcg accgaagatc tcaaaactcc gggtctttca actgtctgac
     6061 tagaccatgt tcgtaacgtc ggacagcagc tttcgttgta ctggtagaat ttctacgtgc
     6121 gaagcacgtg taggcaggtt gaacgacgat ccctgccgat ggatggattg gcacgcggcg
     6181 gaacgctttc gtgatctaca ccacctggat cttcacatat cttcgaaatc gaaaaattaa
     6241 ccaagtcgac ggtatcgata atattctagc tgagggtacc catggccaag ttgaccagtg
     6301 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     6361 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     6421 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     6481 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     6541 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     6601 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgaccga
     6661 cgccgaccaa caccgccggt ccgacgcggc ccgacgggtc cgaggcctcg gagatctggg
     6721 cccatgcggc cgcaacaact acctcgactt tggctgggac actttcagtg aggacaagaa
     6781 gcttcagaag cgtgctatcg aactcaacca gggacgtgcg gcacaaatgg gcatccttgc
     6841 tctcatggtg cacgaacagt tgggagtctc tatccttcct taaaaattta attttcatta
     6901 gttgcagtca ctccgctttg gtttcacagt caggaataac actagctcgt cttcagcgag
     6961 catcacgtgc tataaaaata attataattt aaatttttta atataaatat ataaattaaa
     7021 aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa
     7081 gactctaggg ggatcgccaa caaatactac cttttacctt gctcttcctg ctctcaggta
     7141 ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt
     7201 ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat
     7261 aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt
     7321 tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca
     7381 taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc
     7441 gcgtgtaagt tacaggcaag cgatcctagt acactctata tttttttatg cctcggtaat
     7501 gattttcatt ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat
     7561 tatcacataa tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa
     7621 aaaatgagca ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc
     7681 gtattacaaa tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga
     7741 tagagcactc gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat
     7801 cgcaagtgat taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg
     7861 ccaagcattc cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc
     7921 atcacaccac tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg
     7981 ccgtgcgtgg agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca
     8041 gagcggtggt agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg
     8101 agaaagtagg agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg
     8161 ctagcagaat taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg
     8221 agagtgcgtt caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca
     8281 acgatgttcc ctccaccaaa ggtgttctta tgtagtttta cacaggagtc tggacttgac
     8341 ctctagagtc gacctgcgca tctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9601 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9901 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     9961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10261 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10321 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg
    10381 t
//
