LOCUS       sequence-179765-       10601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector featuring H4 promoter in front of AttR1/2
            sites on episomal vector maintained in the diatom Phaeodactylum 
            tricornutum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Bielinski VA, Bolt TM, Dupont CL, Weyman PD
  TITLE     Episomal tools for RNAi in the diatom Phaeodactylum tricornutum
  JOURNAL   PeerJ Preprints. 2017 5:e2907v1
REFERENCE   2  (bases 1 to 10601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            Preprints. 2017 5:e2907v1"
COMMENT     Sequence Label: pH4-DEST
FEATURES             Location/Qualifiers
     source          1..10601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             70..1260
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(422..441)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             1899..2090
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2084..2106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2321..2692)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2725..2834
                     /note="incP origin of transfer"
     protein_bind    3817..3941
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3966..3971,3972..3989,3990..3996)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4050..4709
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4116..4135)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5051..5356
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5235..5254
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5397..5521)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5773..5791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             6495..6869
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    7175..7678
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7679..7865
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             7866..8528
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(8587..8604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8758..9346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8838..8857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9517..10308,10309..10377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10140..10159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10378..10482)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10550..10568
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(10595..10600,10601,1..16,17..22)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag
       61 gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat
      121 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat
      181 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt
      241 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc
      301 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg
      361 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      421 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      481 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      541 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      601 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc
      661 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
      721 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
      781 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
      841 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
      901 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
      961 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     1021 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     1081 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
     1141 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
     1201 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
     1261 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
     1321 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
     1381 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
     1441 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
     1501 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
     1561 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
     1621 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     1681 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     1741 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     1801 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     1861 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     1921 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     1981 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     2041 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2101 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2161 gcgcgtcagc gggtgttggc gggtgtcggg gcggatcgtc ttgccttgct cgtcggtgat
     2221 gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc
     2281 aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc
     2341 ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca
     2401 gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt
     2461 tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct
     2521 catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg taggccgaca
     2581 ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt
     2641 acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc
     2701 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca
     2761 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt
     2821 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt
     2881 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga
     2941 ccccgaagca gggttatgca gcggaagatg ccattcgcca ttcaggctgc gcaactgttg
     3001 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3061 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaacgacg
     3121 gccagtggca atctcacgca ccaggcgctg gaagggcaac ttgcggatga gaaggtccgt
     3181 ggacttctgg taacgacgga tctcacgcag agcgacggtt ccagggcgat aacggtgggg
     3241 cttcttgact cctccggtag ccggagcgga cttgcgggca gccttggtgg caagctgctt
     3301 gcgcggcgct ttgcctccgg tggatttacg ggcggtttgc ttggttcggg ccattttgac
     3361 ggtttttttt acaagagaag agttcttgaa atttgtgagg ttaaagtgtg tggcttccgc
     3421 cgtagtcaag gagcgtgcgg ttgccgatcg caccggtacg ttctgtagaa atgaacacag
     3481 tgtgttgaat tgaaagtatg gcgcaggtat ggtgtgtgat aagtagcagc cgcgccgaga
     3541 caaacaaact ttggtttcta cgacaatctc tgtagacaag tactagaaac ccgtttgaac
     3601 gagcataaat ctgcaccggc aggccaccag acatcgtttc aacgtaatat tctacgtaac
     3661 cattttatcc caggaaacct acggcctgtg aaccactgaa aggaagcact cacaattcgc
     3721 tctcggcaac aaccgacaat agtcttactc acagtcaata ccgaaaacaa acaacagcca
     3781 acaagtcaag tcgacggtat cgataagctt gatatcacaa gtttgtacaa aaaagctgaa
     3841 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     3901 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     3961 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     4021 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     4081 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     4141 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     4201 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     4261 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     4321 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     4381 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     4441 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     4501 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     4561 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     4621 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     4681 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
     4741 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     4801 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     4861 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     4921 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     4981 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     5041 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     5101 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     5161 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     5221 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     5281 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     5341 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     5401 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     5461 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     5521 tgaattctga gctacctcga ctttggctgg gacactttca gtgaggacaa gaagcttcag
     5581 aagcgtgcta tcgaactcaa ccagggacgt gcggcacaaa tgggcatcct tgctctcatg
     5641 gtgcacgaac agttgggagt ctctatcctt ccttaaaaat ttaattttca ttagttgcag
     5701 tcactccgct ttggtttcac agtcaggaat aacactagct cgtcttcagg accatgatta
     5761 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccta acaggattag
     5821 tgcaattcga gttgaatcac tgggaaaaac attgtcttct tttttatatt atcatttgca
     5881 ttagtgctgc agtcgtagat acttgttggt tgaaagacat cagctgggag ggactggact
     5941 agcgtttggt aaggagacat acctgttaac gttggttgca aaattccatt tcgcgattta
     6001 tgttatctgt aaatcctgat ttgtctggaa ttcttgatac ttccgttttt ttagaggcca
     6061 atgattagca tcggcgattc tcaaaatagc attttcgaca tgcggtgctg atttcataaa
     6121 catagacaac gcttttacat gtaaaagtaa cttgcggact tggaacagtg ctctgttttt
     6181 ggtgtgaacg taactcagca atatttctgt gctagcaagg ttttttatga tcgaccgaag
     6241 atctcaaaac tccgggtctt tcaactgtct gactagacca tgttcgtaac gtcggacagc
     6301 agctttcgtt gtactggtag aatttctacg tgcgaagcac gtgtaggcag gttgaacgac
     6361 gatccctgcc gatggatgga ttggcacgcg gcggaacgct ttcgtgatct acaccacctg
     6421 gatcttcaca tatcttcgaa atcgaaaaat taaccaagtc gacggtatcg ataatattct
     6481 agctgagggt acccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6541 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6601 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     6661 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     6721 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     6781 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     6841 tgcacttcgt ggccgaggag caggactgac cgacgccgac caacaccgcc ggtccgacgc
     6901 ggcccgacgg gtccgaggcc tcggagatct gggcccatgc ggccgcaaca actacctcga
     6961 ctttggctgg gacactttca gtgaggacaa gaagcttcag aagcgtgcta tcgaactcaa
     7021 ccagggacgt gcggcacaaa tgggcatcct tgctctcatg gtgcacgaac agttgggagt
     7081 ctctatcctt ccttaaaaat ttaattttca ttagttgcag tcactccgct ttggtttcac
     7141 agtcaggaat aacactagct cgtcttcagc gagcatcacg tgctataaaa ataattataa
     7201 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
     7261 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
     7321 taccttttac cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
     7381 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
     7441 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
     7501 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
     7561 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
     7621 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatcct
     7681 agtacactct atattttttt atgcctcggt aatgattttc attttttttt tccacctagc
     7741 ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta tacattatat
     7801 aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga taaacgaagg
     7861 caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc aagattcaga
     7921 ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc ccagaaaaag
     7981 aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc cacacaggta
     8041 tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg tcgctaatcg
     8101 ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac tgcgggattg
     8161 ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa aggtttggat
     8221 caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt tcgaacaggc
     8281 cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga
     8341 tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc cacgttgatt
     8401 gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct cttgcggttg
     8461 ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc aaaggtgttc
     8521 ttatgtagtt ttacacagga gtctggactt gacctctaga gtcgacctgc gcatctcttc
     8581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     9841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
    10141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10501 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10561 cacgaggccc tttcgtcttc aagaattctc atgtttgaca g
//
