LOCUS       sequence-179774-        3264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis
            to purify specific genomic regions of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     enChIP systems using different CRISPR orthologues and epitope tags.
  JOURNAL   BMC Res Notes. 2018 Feb 27;11(1):154. doi: 
            10.1186/s13104-018-3262-4.
  PUBMED    29482606
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2018-02-27"; volume: "11(1):154. doi"; pages: " 
            10.1186/s13104-018-3262-4"
COMMENT     Sequence Label: Sa-dCas9-NLS-3xFLAG/pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..3264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3169..3189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3196..3261
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgggcaaac ggaactacat cctggggctt gccattggga taaccagcgt tggctacgga
       61 attattgatt atgagacacg cgatgtgatt gacgccgggg ttaggctgtt caaagaggcc
      121 aacgttgaaa acaacgaggg aagacggagt aagcgcggag caagaagact caagcgcaga
      181 cggagacatc ggattcagag ggtgaaaaag ctgctcttcg attacaatct cctgaccgat
      241 catagtgagc tgagcggaat caacccctac gaggcgcgag tgaaagggct ttcccagaag
      301 ctgtccgaag aggagttctc cgccgcgttg ctgcacctgg ccaaacggag gggggttcac
      361 aatgtaaacg aagtggagga ggacacgggc aatgaactta gtacgaaaga acagatcagt
      421 aggaactcta aggctctcga agagaaatac gtcgctgagt tgcagcttga gagactgaaa
      481 aaagacggcg aagtacgcgg atctattaat aggttcaaga cttcagatta cgtaaaggaa
      541 gccaagcagc tcctgaaagt acagaaagcg taccatcagc tcgatcagag cttcatcgat
      601 acctacatag atttgctgga gacacggagg acatactacg agggcccagg ggaaggatct
      661 ccttttgggt ggaaggacat caaggaatgg tacgagatgc ttatgggaca ttgtacatat
      721 tttccggagg agctcaggag cgtcaagtac gcctacaatg ccgacctgta caatgccctc
      781 aatgacctca ataacctcgt gattaccagg gacgagaacg agaagctgga gtactatgaa
      841 aagttccaga ttatcgagaa tgtgtttaag cagaagaaga agccgacact taagcagatt
      901 gcaaaggaaa tcctcgtgaa tgaggaagat atcaagggat acagagtgac aagtacaggc
      961 aagcccgagt tcacaaatct gaaggtgtac cacgatatta aggacataac cgcacgaaag
     1021 gagataatcg aaaacgctga gctcctcgat cagatcgcaa aaattcttac catctaccag
     1081 tctagtgagg acattcagga ggaactgact aatctgaaca gtgagctcac ccaagaggaa
     1141 attgagcaga tttcaaacct gaaaggctac accgggacgc acaatctgag cctcaaagca
     1201 atcaacctca ttctggatga actttggcac acaaatgaca accaaattgc catattcaac
     1261 cgcctgaaac tggtgccaaa aaaagtggat ctgtcacagc aaaaggaaat ccctacaacc
     1321 ttggttgacg attttattct gtcccccgtt gtcaagcgga gcttcatcca gtcaatcaag
     1381 gtgatcaatg ccatcattaa aaaatacgga ttgccaaacg atataattat cgagcttgca
     1441 cgagagaaga actcaaagga cgcccagaag atgattaacg aaatgcagaa gcgcaaccgc
     1501 cagacaaacg aacgcataga ggaaattata agaacaaccg gcaaagagaa tgccaagtat
     1561 ctgatcgaga aaatcaagct gcacgacatg caagaaggca agtgcctgta ctctctggaa
     1621 gctatcccac tcgaagatct gctgaataat ccattcaatt acgaggtgga cgccatcatc
     1681 cctagatccg taagctttga caattccttc aataacaaag ttctggttaa acaggaggaa
     1741 aattctaaaa aagggaaccg gaccccgttc cagtacctga gctccagtga cagcaagatt
     1801 agctacgaga cttttaagaa acatattctg aatctggcca aaggcaaagg caggatcagc
     1861 aagaccaaga aggagtacct cctcgaagaa cgcgacatta acagatttag tgtgcagaaa
     1921 gatttcatca accgaaacct tgtcgatact cggtacgcca cgagaggcct gatgaatctc
     1981 ctcaggagct acttccgcgt caataatctg gacgttaaag tcaagagcat aaatggggga
     2041 ttcaccagct ttctgaggag aaagtggaag tttaagaagg aacgaaacaa aggatacaag
     2101 caccatgctg aggatgcttt gatcatcgct aacgcggact ttatctttaa ggaatggaaa
     2161 aagctggata aggcaaagaa agtgatggaa aaccagatgt tcgaggagaa gcaggcagag
     2221 tcaatgcctg agatcgagac agagcaggaa tacaaggaaa ttttcatcac ccctcatcag
     2281 attaaacaca taaaggactt caaagactat aaatactctc atagggtgga caaaaaaccc
     2341 aatcgcgagc tcattaatga caccctgtac tcaacacgga aggatgataa aggtaatacc
     2401 ttgattgtga ataatcttaa tggattgtat gacaaagata acgacaagct caagaagctg
     2461 atcaacaagt ctccagagaa gctccttatg tatcaccacg acccacagac ttatcagaaa
     2521 ttgaaactga tcatggagca atacggggat gagaagaacc cactctacaa atattatgag
     2581 gaaacaggta attacctgac caagtactcc aagaaggata acggaccagt gatcaaaaag
     2641 ataaagtact atggcaacaa acttaatgcg catttggaca taactgacga ttaccccaat
     2701 tctcgaaaca aggttgtgaa gctctccctg aagccttata gatttgacgt gtacctggat
     2761 aatggggttt ataaattcgt caccgtgaaa aatctggacg tgatcaaaaa ggagaactat
     2821 tatgaagtaa actcaaagtg ctatgaggag gcgaagaagc tgaagaagat ctccaatcag
     2881 gccgagttca tcgcttcctt ctataataac gatctcatca agatcaatgg agagctttat
     2941 cgcgtcattg gtgtgaacaa tgacttgctg aacaggatcg aagtcaatat gatagacatt
     3001 acctaccggg agtatctcga aaacatgaat gataaacggc cgcctcgcat catcaagaca
     3061 atcgcatcta aaactcagtc aataaaaaag tactctaccg atatcctggg gaatctctat
     3121 gaagtgaagt caaagaagca cccacaaatc attaaaaaag gtggatcccc caagaagaag
     3181 aggaaagtct cgagcgacta caaagaccat gacggtgatt ataaagatca tgacatcgat
     3241 tacaaggatg acgatgacaa gtaa
//
