LOCUS       sequence-179822-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  titin (NM_133378, nt 99776 to 100510) in pBSSK+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  TITLE     Shiang Lab CMYA5 project plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TTN/pBSSK+
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..815)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..815)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(852..872)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(852..870)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(891..907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(891..907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(904..926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    915..931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(939..945,946..963,964..969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    984..1005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgaggtcgac ggtatcgata agcttgattc catgggctcc agaaatgtat actcccttga
       61 aatccgaaat gcatcagtca gcgacagtgg aaagtacaca attaaggcca aaaatttccg
      121 tggccagtgt tcagctacag cttccttaat ggtccttcct ctagttgaag aaccttccag
      181 agaggtagta ttgagaacaa gtggtgacac aagcttgcaa ggaagcttct cgtctcagtc
      241 agtccaaatg tctgcctcca agcaggaggc ctccttcagc agtttcagca gcagcagtgc
      301 tagcagcatg actgagatga aatttgcaag catgtctgcc caaagcatgt cctccatgca
      361 agagtccttt gtagaaatga gttccagcag ctttatggga atatctaata tgacacaact
      421 ggaaagctca actagtaaaa tgcttaaagc aggcataaga ggaattccgc ctaaaattga
      481 agctcttcca tctgatatca gcattgatga aggcaaagtt ctaacagtag cctgtgcttt
      541 cacgggtgag cctaccccag aagtaacatg gtcctgtggt ggaagaaaaa tccacagtca
      601 agaacagggg aggttccaca ttgaaaacac agatgacctg acaaccctga tcatcatgga
      661 cgtacagaaa caagatggtg gactttatac cctgagttta gggaatgaat ttggatctga
      721 ctctgccact gtgaatatac atattcgatc catttaagag ggcctgtgcg aattcaatcg
      781 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
      841 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
      901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1021 ctgc
//
