LOCUS       sequence-179827-        2881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMYA5 (NM_153610, nt 11648 to 12279) in pENTR3C, Gateway Entry 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  TITLE     Shiang Lab CMYA5 project plasmids (will be published)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMYA5tnC/pENTR3C
FEATURES             Location/Qualifiers
     source          1..2881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1110..1209)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1321..1340)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1332..2141
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1399..1418)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2231..2819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2720..2739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt tcatgggact ccagctgaaa
      481 gttaacctcc aacccaatga taactacttt ttctatgtga gggccatcaa tgcatttggg
      541 acaagtgaac agagtgaagc tgctctcatc tccaccagag gaaccagatt tctcttgttg
      601 agagaaacag ctcatcctgc tctacacatt tcctcaagtg ggacagtgat cagctttggt
      661 gagaggagac ggctgacgga aatcccgtca gtgctgggtg aggagctgcc ttcctgtggc
      721 cagcattact gggaaaccac agtcacagac tgcccagcat atcgactcgg catctgctcc
      781 agctcggctg tgcaggcagg tgccctagga caaggggaga cctcatggta catgcactgc
      841 tctgagccac agagatacac atttttctac agtggtattg tgagtgatgt tcatgtgact
      901 gagcgtccag ccagagtggg catcctgctg gactacaaca accagagact tatcttcatc
      961 aacgcagaga gcgagcagtt gctcttcatc atcaggcaca ggtttaatga gggtgtccac
     1021 cctgcctttg ccctggagaa acctggaaaa tgtactttgc acctggggat agagcccccg
     1081 gattctgtaa ggcacaagga attatctaga cccagctttc ttgtacaaag ttggcattat
     1141 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1201 cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg
     1261 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     1321 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     1381 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     1441 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     1501 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     1561 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     1621 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     1681 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     1741 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     1801 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     1861 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     1921 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     1981 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2041 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2101 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     2161 tgtaacatta ttcagattgg gccccgttcc actgagcgtc agaccccgta gaaaagatca
     2221 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2281 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2341 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2401 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2461 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2521 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2581 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2641 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2701 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2761 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2821 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2881 t
//
