LOCUS       sequence-179836-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  selectable HDR vector to introduce silent mutation (position 700) in
            mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA 
            vectors enabling generation of hemizygous K700E mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gupta AK, Murthy T, Paul KV, Ramirez O, Fisher JB, Rao S, Rosenberg 
            AB, Seelig G, Minella AC, Pillai MM
  TITLE     Degenerate minigene library analysis enables identification of 
            altered branch point utilization by mutant splicing factor 3B1 
            (SF3B1).
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193340. doi: 
            10.1093/nar/gky1161.
  PUBMED    30462273
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193340. doi: 10.1093/nar/gky1161."
COMMENT     Sequence Label: pL452(hygro)-Sf3b1-K700K
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acattaaatt cctagaaaag gaagtttgtc ttttgatggg ttgaggatta atggtactaa
       61 cttaagtggc tgttgtcact tttctcttta ggctgctggt ctggctacta tgatttctac
      121 catgagacct gatatagata acatggatga atatgtccga aacacaacag ctagagcttt
      181 tgctgtcgta gcctctgccc tgggcatccc ttctttattg cctttcttaa aagctgtgtg
      241 caaaagcaag aagtcttggc aagcgaggca cactggtatt aagattgtac aacagatagc
      301 cattctcatg ggctgtgcta tcttgccaca tctcagaagt ttggttgaaa tcattgaaca
      361 tggtaagtgg tgtatctttt atagatcagc tgtcctgatg gggagaggtg tatgtgtaat
      421 taaatgaaag acttctcacg caaattttga aatggggttt gtaatatttt ctgttaacaa
      481 attaccattg cctaaatttt atgtactata aaaaaattat tgcagtttgg atacatggtt
      541 agatacattg ctaaaatttg gattgtatta tgaaaattag gtgtgggtac ctactcataa
      601 ccatgtctga ttgtataggt ctcgtggatg agcagcagaa ggttcggacc atcagtgctc
      661 tggccattgc tgctttggct gaggcagcaa ctccttatgg tattgagtct tttgattctg
      721 tcctgaaacc gctttggaaa ggtatccgcc aacacagagg aaaggtgaat cgctcattag
      781 atcagttttt gttgctagta
//
