LOCUS       sequence-179837-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  selectable HDR vector to introduce silent mutation (position 700) in
            mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA 
            vectors enabling generation of hemizygous K700E mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gupta AK, Murthy T, Paul KV, Ramirez O, Fisher JB, Rao S, Rosenberg 
            AB, Seelig G, Minella AC, Pillai MM
  TITLE     Degenerate minigene library analysis enables identification of 
            altered branch point utilization by mutant splicing factor 3B1 
            (SF3B1).
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193340. doi: 
            10.1093/nar/gky1161.
  PUBMED    30462273
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193340. doi: 10.1093/nar/gky1161."
COMMENT     Sequence Label: pL452(hygro)-Sf3b1-K700K
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agataaccga aacttaattt actaacgtat atatttttaa atagggtctg gctgctttct
       61 tgaaggctat tgggtatctt attcctctta tggatgcaga atatgccaac tactatacta
      121 gagaagtgat gttaatcctc attcgagaat ttcagtctcc tgatgaagaa atgaagaaaa
      181 ttgttttgaa ggtaagtaat tagttgctca gtttgtgggt atgtgcatgt tttaggaagt
      241 tcagagagat ctttataaca ttaaaaacaa atcaaaccta tttttgtact gtaaaagtta
      301 ttacagcttg gatacatggt tagatacatt gctaaaattt ggattgcatt acgaaaatta
      361 ggtattggta cctactcata actgtgttgg attgttttgt gttttgccag tgctgcattt
      421 gatatatata tttctttttt attaacctta taggtggtga aacagtgttg tggaactgat
      481 ggtgtagaag caaactacat taaaacagag attcttccac ctttctttaa acacttttgg
      541 caacacagaa tggctttgga tagaagaaat taccgacagg tgacctttta ttgctaaact
      601 ttgattctca taaatctttt tgactaattc tttacctact tagaatgcat attacatgct
      661 ataatttgct ttgactgtct taaaatttat acttgtatgt actttactgt gacaagagat
      721 ggaattttta aagtccattc tttctgtttg gtctgtctgt ctgagatagg gtctcactaa
      781 gtagcctaga aatcactctg
//
