LOCUS       sequence-179902-        2175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal 
            His6-tag (TEV cleavable) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ludewig H, Czekster CM, Oueis E, Munday ES, Arshad M, Synowsky SA, 
            Bent AF, Naismith JH
  TITLE     Characterization of the Fast and Promiscuous Macrocyclase from Plant
            PCY1 Enables the Use of Simple Substrates.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):801-811. doi: 
            10.1021/acschembio.8b00050. Epub 2018 Feb 12.
  PUBMED    29377663
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.8b00050"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "801-811"
COMMENT     Sequence Label: pJ414-PCY1:S495A
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctacca gcggtttcag caagccgctg cattatccgc cggtccgccg cgatgaaacc
       61 gttgttgacg attactttgg tgtgaaagtc gcggacccat accgctggtt ggaggacccg
      121 aactctgagg aaacgaaaga atttgttgat aaccaagaaa aactggcaaa ctcggtcctg
      181 gaagagtgtg agctgattga caagtttaag caaaagatta tcgatttcgt gaatttcccg
      241 cgttgcggtg tgccgttccg ccgcgccaac aaatactttc acttctacaa cagcggcctg
      301 caggcgcaaa acgtctttca gatgcaagat gatttggacg gtaagcctga agttctgtac
      361 gatccgaatc tgcgcgaggg cggtcgcagc ggcctgtcct tgtacagcgt cagcgaagat
      421 gccaagtact ttgcctttgg tattcatagc ggcctgaccg aatgggtgac catcaagatt
      481 ctgaaaactg aagatcgttc ttatttgccg gataccctgg aatgggtgaa gttcagcccg
      541 gctatctgga cccacgataa caaaggtttc ttttactgtc cgtatccgcc actgaaagag
      601 ggcgaggacc acatgactcg ctccgcagtt aaccaagagg ctcgttatca cttcctgggc
      661 acggatcaga gcgaagatat cctgctgtgg cgcgatctgg agaatccggc gcaccacctg
      721 aaatgtcaga tcaccgacga tggcaagtat tttctgctgt atatcttgga cggttgtgat
      781 gatgccaata aagtttactg cttggatctg acgaagctgc cgaacggcct ggagagcttt
      841 cgtggtcgcg aagattcagc accgttcatg aagctgattg atagcttcga cgcgtcgtac
      901 acggcgattg ctaatgatgg ttccgtgttc accttccaga cgaacaaaga cgcaccgcgt
      961 aagaaactgg ttcgcgtgga tctgaataat ccgagcgttt ggacggacct ggtgcctgag
     1021 agcaaaaaag atctgctgga gagcgcccac gcagtgaatg aaaatcaact gattctgcgt
     1081 tacttgagcg atgtgaaaca tgttctggaa attcgtgacc tggagagcgg tgcactgcaa
     1141 caccgtctgc cgatcgacat tggcagcgtt gacggtatta cggcgcgtcg tcgcgactcc
     1201 gtggttttct ttaagttcac cagcatcctg accccgggca ttgtttatca gtgcgacctg
     1261 aaaaacgacc cgacccagct gaagatcttc cgtgagagcg tggtcccgga cttcgaccgc
     1321 tccgaatttg aagtcaaaca agttttcgtt ccgagcaagg acggtaccaa aatcccgatc
     1381 ttcattgccg cgcgtaaggg catcagcttg gacggcagcc acccgtgcga gatgcacggt
     1441 tatggcggtt ttggcattaa tatgatgcca acgttcagcg cagcgcgtat tgtctttctc
     1501 aagcatctgg gtggcgtctt ttgcctggcg aatatccgtg gcggcggcga atacggtgag
     1561 gaatggcaca aggccggctt tcgtgacaaa aagcaaaatg tttttgacga tttcatctcg
     1621 gcggcggagt atctgatcag cagcggttat accaaggcac gtcgtgtggc gatcgagggt
     1681 ggtagcaatg gtggcctgct ggtcgcggcg tgtattaacc aacgcccgga cttgtttggt
     1741 tgcgctgagg ccaactgcgg tgtcatggat atgctgcgtt ttcataagtt caccctgggt
     1801 tacctgtgga ctggtgatta cggttgtagc gataaggaag aagaattcaa gtggctgatt
     1861 aaatactctc caattcacaa cgtgcgtcgt ccgtgggagc agccgggtaa cgaagaaacg
     1921 caatatcctg cgaccatgat tctgaccgcg gaccacgacg accgcgtagt cccgctgcac
     1981 tctttcaaac tgctggcgac catgcaacac gtgctgtgca ctagcttaga ggactctccg
     2041 cagaagaatc cgattatcgc acgtattcag cgtaaagctg cccattacgg tcgtgcgacg
     2101 atgacgcaga tcgcagaagt ggcggaccgc tacggtttca tggcgaaagc cctggaagca
     2161 ccgtggatcg attga
//
