LOCUS       sequence-179918-       11463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector featuring 49202 promoter in front of 
            AttR1/2 sites on episomal vector maintained in the diatom 
            Phaeodactylum tricornutum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Bielinski VA, Bolt TM, Dupont CL, Weyman PD
  TITLE     Episomal tools for RNAi in the diatom Phaeodactylum tricornutum
  JOURNAL   PeerJ Preprints. 2017 5:e2907v1
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            Preprints. 2017 5:e2907v1"
COMMENT     Sequence Label: p49202-DEST
FEATURES             Location/Qualifiers
     source          1..11463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(833..1495)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(1496..1682)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     misc_feature    1683..2186
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(2492..2866)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3570..3588)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3840..3964
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4005..4310)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4107..4126)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4652..5311)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5226..5245
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(5365..5371,5372..5389,5390..5395))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(5420..5544)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7096..7113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7096..7112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7105..7127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     oriT            7389..7498
                     /note="incP origin of transfer"
     CDS             7531..7902
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     8117..8139
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8133..8324)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8963..10153)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     9782..9801
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(10201..10206,10207..10223,10224..10229))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(10256..10274)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10342..10446
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10447..10515,10516..11307)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10665..10684)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagatgc gcaggtcgac tctagaggtc aagtccagac tcctgtgtaa aactacataa
      841 gaacaccttt ggtggaggga acatcgttgg taccattggg cgaggtggct tctcttatgg
      901 caaccgcaag agccttgaac gcactctcac tacggtgatg atcattcttg cctcgcagac
      961 aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct ttcaagaaaa tgcgggatca
     1021 tctcgcaaga gagatctcct actttctccc tttgcaaacc aagttcgaca actgcgtacg
     1081 gcctgttcga aagatctacc accgctctgg aaagtgcctc atccaaaggc gcaaatcctg
     1141 atccaaacct ttttactcca cgcacggccc ctagggcctc tttaaaagct tgaccgagag
     1201 caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca atgcactcaa
     1261 cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc agaaacccta
     1321 tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact gcttctgcct
     1381 ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa gagatcgcaa
     1441 tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct gtcatctttg
     1501 ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt
     1561 atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc
     1621 gctaggtgga aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat agagtgtact
     1681 aggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg
     1741 aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa
     1801 gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa
     1861 caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa atagatatac
     1921 attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac
     1981 agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag gtaaaaggta
     2041 gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt
     2101 ctttaatttc tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa
     2161 ttataattat ttttatagca cgtgatgctc gctgaagacg agctagtgtt attcctgact
     2221 gtgaaaccaa agcggagtga ctgcaactaa tgaaaattaa atttttaagg aaggatagag
     2281 actcccaact gttcgtgcac catgagagca aggatgccca tttgtgccgc acgtccctgg
     2341 ttgagttcga tagcacgctt ctgaagcttc ttgtcctcac tgaaagtgtc ccagccaaag
     2401 tcgaggtagt tgttgcggcc gcatgggccc agatctccga ggcctcggac ccgtcgggcc
     2461 gcgtcggacc ggcggtgttg gtcggcgtcg gtcagtcctg ctcctcggcc acgaagtgca
     2521 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     2581 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     2641 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     2701 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     2761 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     2821 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatgggt accctcagct
     2881 agaatattat cgataccgtc gacttggtta atttttcgat ttcgaagata tgtgaagatc
     2941 caggtggtgt agatcacgaa agcgttccgc cgcgtgccaa tccatccatc ggcagggatc
     3001 gtcgttcaac ctgcctacac gtgcttcgca cgtagaaatt ctaccagtac aacgaaagct
     3061 gctgtccgac gttacgaaca tggtctagtc agacagttga aagacccgga gttttgagat
     3121 cttcggtcga tcataaaaaa ccttgctagc acagaaatat tgctgagtta cgttcacacc
     3181 aaaaacagag cactgttcca agtccgcaag ttacttttac atgtaaaagc gttgtctatg
     3241 tttatgaaat cagcaccgca tgtcgaaaat gctattttga gaatcgccga tgctaatcat
     3301 tggcctctaa aaaaacggaa gtatcaagaa ttccagacaa atcaggattt acagataaca
     3361 taaatcgcga aatggaattt tgcaaccaac gttaacaggt atgtctcctt accaaacgct
     3421 agtccagtcc ctcccagctg atgtctttca accaacaagt atctacgact gcagcactaa
     3481 tgcaaatgat aatataaaaa agaagacaat gtttttccca gtgattcaac tcgaattgca
     3541 ctaatcctgt taggtaccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     3601 taatcatggt cctgaagacg agctagtgtt attcctgact gtgaaaccaa agcggagtga
     3661 ctgcaactaa tgaaaattaa atttttaagg aaggatagag actcccaact gttcgtgcac
     3721 catgagagca aggatgccca tttgtgccgc acgtccctgg ttgagttcga tagcacgctt
     3781 ctgaagcttc ttgtcctcac tgaaagtgtc ccagccaaag tcgaggtagc tcagaattca
     3841 ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     3901 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     3961 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
     4021 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     4081 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     4141 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
     4201 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     4261 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
     4321 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     4381 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     4441 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
     4501 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
     4561 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
     4621 atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt
     4681 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     4741 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     4801 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     4861 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     4921 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     4981 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     5041 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     5101 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     5161 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     5221 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     5281 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     5341 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
     5401 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
     5461 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     5521 ttcagctttt ttgtacaaac ttgtgatatc aagcttatcg ataccgtcga cttgacttgg
     5581 tggtgcggaa agaggcgtag gacaaccaaa aaagctggtg caatttgcgg cgttttggtt
     5641 tttatcagag agagttgcaa tgttttggac aacgcgtttt tggcgttgga ttcggttcgt
     5701 gttcatcgag ttctcgtgcc gcggagcatc tgggcgttct cgttctcagt catggcattt
     5761 tcgaagcatg tccatcaact tgattgcagg gagctcttat gtctcggcac tccgtaatct
     5821 caccatgccc ctccacatag tgaggatcga actctgctct taacaattga tatatttgaa
     5881 tagattggtt ggaccacata gatgccttcc gaaagttgag ctttctgtac aatctcggag
     5941 aattcgctcg agcaatccaa cgacaaaaaa ccctcaccag cgactcggtc gtacatagac
     6001 tacaattact ttttgcggat tcctcggacc aacgaattcg gattgtttga aaccatgaat
     6061 cggagttacg acattggaca ttcctgatgc tcaatgagta atatatataa agagcgaagt
     6121 gtagcatttc tgtaatcgtt ttaacgatcg ccgcaaatga gaacctcaaa agcgacccca
     6181 tagtgcacgc ctagtgaaat agggattctt gcgaattcct tgactgtgga ccaacgaatt
     6241 cggattgttt gaaaccataa attggagcta ggacgttgga aattcgtact actcactgac
     6301 tctgagtaat acagatataa agagagaagt aaggcatttc tctaattgtt ttaacgatcg
     6361 ctggaaatga gaactgactg ctccgcgcat tgtatggtac caaattttga acaaggagag
     6421 tgtcatgacc aatgacttta atatgatcac tggagacttt acataggaag ccaaataggg
     6481 cgtatcaagc tgtaaactca cgaggggaag gtcggttgac gcgaccgagt agggaaactt
     6541 ttgaatcgga gcgcgtccgc aacgatttca ctgtcaacaa aaatttgtct tcaatcgtgg
     6601 tgtcaatgac tgtatgagaa gcccaatgac caatagatct tggaacgatg ccatctgcct
     6661 ttcgtttgaa tggcccacag agatcccccc gactagcatc cgtccgttcg ctcgttggga
     6721 ggatctgggc acccacgtgc tttctggtag atggcgagcg caagattgac ttttactgtt
     6781 aacctatcct gcaaattcag aatgatcaag atccaaccaa gctcaagttt cgagttctca
     6841 attcaatggt tgccatcttc tctatcccag cgagagacgg tcacgacccc cctcactgtc
     6901 aagtatatac ttatatctaa atagaatatc cttgcagatt caagaagtct ttgctggcta
     6961 gaggtcttga agattggaaa gtgtcaaatt ccacgtcact accgcagcct gttcttccac
     7021 tacgagctta cgcactggaa taaccgggtg cggtagtgac gtgggatctg acgtaccgtt
     7081 tggacgggtg cgatcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7141 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7201 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcatcttcc
     7261 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     7321 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     7381 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     7441 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct
     7501 accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag
     7561 ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag
     7621 gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa
     7681 atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg
     7741 ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc
     7801 ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc
     7861 aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa
     7921 cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga
     7981 cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagacgatcc gccccgacac
     8041 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8101 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8161 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     8221 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     8281 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     8341 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     8401 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     8461 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     8521 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     8581 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     8641 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     8701 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     8761 aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc
     8821 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     8881 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     8941 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     9001 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
     9061 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc gaagttaggc
     9121 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     9181 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     9241 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     9301 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     9361 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     9421 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     9481 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     9541 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat
     9601 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     9661 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     9721 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     9781 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     9841 cgatgcgtcc ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga
     9901 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
     9961 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
    10021 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
    10081 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
    10141 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
    10201 attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
    10261 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    10321 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10381 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10441 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10501 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10561 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10621 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10681 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10741 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10801 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10861 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10921 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10981 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    11041 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    11101 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    11161 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11221 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11281 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11341 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11401 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11461 aag
//
