LOCUS       sequence-179922-       10913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector featuring nitrate reductase (NR) promoter
            in front of AttR1/2 sites on episomal vector maintained in the 
            diatom Phaeodactylum tricornutum..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Bielinski VA, Bolt TM, Dupont CL, Weyman PD
  TITLE     Episomal tools for RNAi in the diatom Phaeodactylum tricornutum
  JOURNAL   PeerJ Preprints. 2017 5:e2907v1
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            Preprints. 2017 5:e2907v1"
COMMENT     Sequence Label: pNR-DEST
FEATURES             Location/Qualifiers
     source          1..10913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1372..1563
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1557..1579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1794..2165)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2198..2307
                     /note="incP origin of transfer"
     primer_bind     2569..2591
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2583..2600
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2584..2600
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3322..3446
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3471..3476,3477..3494,3495..3501)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3555..4214
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3621..3640)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4556..4861
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4740..4759
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4902..5026)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5558..5576
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             6280..6654
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    6960..7463
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7464..7650
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             7651..8313
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     primer_bind     complement(8372..8389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8543..9131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8623..8642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9302..10093,10094..10162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9925..9944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10163..10267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10335..10353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(10380..10385,10386..10402,10403..10408)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(10456..10913,1..733)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(10808..10827)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
       61 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc
      121 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
      181 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
      241 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
      301 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
      361 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
      421 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
      481 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
      541 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct
      601 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
      661 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
      721 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
      781 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
      841 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc
      901 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      961 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     1021 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     1081 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     1141 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     1201 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     1261 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     1321 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     1381 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     1441 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     1501 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     1561 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1621 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcggatc gtcttgcctt
     1681 gctcgtcggt gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg
     1741 ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc
     1801 tctgccctcg ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg
     1861 atcttcgcca gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg
     1921 gtgagccaga gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc
     1981 tcgcggacgt gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc
     2041 aggtaggccg acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg
     2101 tctggaaggc agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca
     2161 gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga
     2221 ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg
     2281 ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct
     2341 acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa
     2401 atcgctataa tgaccccgaa gcagggttat gcagcggaag atgccattcg ccattcaggc
     2461 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2521 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2581 gttgtaaaac gacggccagt gagcgaacag aagacccaat cgtggagcag tcatcatcat
     2641 tgtggaaatg tgtcctagac tgttgcagac agtcaccaac ggaatgcttt cgttaagagc
     2701 aaggcgacaa cggttccttc gttgagatgg tggttccact gtgcaggtca cgtagtgaag
     2761 tgatgttgcg gtagtctgca atcccgctag atacacgtca ttcgtgaaag gcctgttgtt
     2821 ttcatgtccg ggtctgcacg ggcactagaa ggaaggaagc aaagacgaac ggaagtgact
     2881 gtaaacgaga agtgcacgaa gccttttctt gtgacgtcac aacccgaata gccctacgtg
     2941 gcgtgcgact ttccggcact ttggatatcg ttgccctata tgtattgggt gtatacatgt
     3001 cgtattccaa ctacgacaga aaacagtcag ttacgggtat tcgaacttcc ggcacctgca
     3061 gcgaggagtt ttgtgtccgt cgcacatcct ccgtgctttc ggcacctacg taggcggcaa
     3121 acttcctgcc ttccgccccc gccttgcggc attccggctc cgggccagaa ttgcccgggt
     3181 gttcacaatt ttgcctcctc acgaaaaaac gttctacttt gtattttggt cgggtttcgg
     3241 atccttccag caaccattct cattcaaagt caccacttgt gcgaacgaag tcaagtcgac
     3301 ggtatcgata agcttgatat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     3361 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     3421 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
     3481 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
     3541 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     3601 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     3661 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     3721 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     3781 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     3841 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     3901 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     3961 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     4021 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     4081 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     4141 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     4201 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
     4261 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     4321 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     4381 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     4441 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     4501 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     4561 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     4621 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     4681 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     4741 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     4801 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     4861 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     4921 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     4981 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgaat tctgagaagt
     5041 tcttgactga ttgtcatatc aattttgcac tttacaatac attttcaata gcaatttact
     5101 ttaagactac cgcagttttt ttcttacatt agatgaacat aaaacgacga tgaggatcac
     5161 tgttagggaa acgatgaact acgctgagga cttcctatta ttcgtccttt gatggcgtgg
     5221 ttgttctttg gtttgcggcc gagcgcaacg accaaggcct gtaccaaaaa cgaagttgat
     5281 aatcagatat ttgagttgta acaagaaact aaagcattat tttagcagta gcacgaatca
     5341 ggatctaaaa tgaacgcaat tgcatggttg atgtctaagc tgcgttagaa cttagaaatt
     5401 gaagagaaca cgcccttcga agacgcccgc cttcgtcagg aagtctttca cagcttcagt
     5461 catgcctttc ataccacaaa gcaaagctcc tgaattccga ggaatatcga ctccgtcttc
     5521 ctctaaagca gtctgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     5581 aaaagctggt acctaacagg attagtgcaa ttcgagttga atcactggga aaaacattgt
     5641 cttctttttt atattatcat ttgcattagt gctgcagtcg tagatacttg ttggttgaaa
     5701 gacatcagct gggagggact ggactagcgt ttggtaagga gacatacctg ttaacgttgg
     5761 ttgcaaaatt ccatttcgcg atttatgtta tctgtaaatc ctgatttgtc tggaattctt
     5821 gatacttccg tttttttaga ggccaatgat tagcatcggc gattctcaaa atagcatttt
     5881 cgacatgcgg tgctgatttc ataaacatag acaacgcttt tacatgtaaa agtaacttgc
     5941 ggacttggaa cagtgctctg tttttggtgt gaacgtaact cagcaatatt tctgtgctag
     6001 caaggttttt tatgatcgac cgaagatctc aaaactccgg gtctttcaac tgtctgacta
     6061 gaccatgttc gtaacgtcgg acagcagctt tcgttgtact ggtagaattt ctacgtgcga
     6121 agcacgtgta ggcaggttga acgacgatcc ctgccgatgg atggattggc acgcggcgga
     6181 acgctttcgt gatctacacc acctggatct tcacatatct tcgaaatcga aaaattaacc
     6241 aagtcgacgg tatcgataat attctagctg agggtaccca tggccaagtt gaccagtgcc
     6301 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     6361 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     6421 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     6481 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     6541 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     6601 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgaccgacg
     6661 ccgaccaaca ccgccggtcc gacgcggccc gacgggtccg aggcctcgga gatctgggcc
     6721 catgcggccg caacaactac ctcgactttg gctgggacac tttcagtgag gacaagaagc
     6781 ttcagaagcg tgctatcgaa ctcaaccagg gacgtgcggc acaaatgggc atccttgctc
     6841 tcatggtgca cgaacagttg ggagtctcta tccttcctta aaaatttaat tttcattagt
     6901 tgcagtcact ccgctttggt ttcacagtca ggaataacac tagctcgtct tcagcgagca
     6961 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
     7021 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
     7081 ctctaggggg atcgccaaca aatactacct tttaccttgc tcttcctgct ctcaggtatt
     7141 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
     7201 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
     7261 atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc
     7321 ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata
     7381 aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc
     7441 gtgtaagtta caggcaagcg atcctagtac actctatatt tttttatgcc tcggtaatga
     7501 ttttcatttt ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta
     7561 tcacataatg aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa
     7621 aatgagcagg caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt
     7681 attacaaatg aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata
     7741 gagcactcga tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg
     7801 caagtgatta acgtccacac aggtataggg tttctggacc atatgataca tgctctggcc
     7861 aagcattccg gctggtcgct aatcgttgag tgcattggtg acttacacat agacgaccat
     7921 cacaccactg aagactgcgg gattgctctc ggtcaagctt ttaaagaggc cctaggggcc
     7981 gtgcgtggag taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga
     8041 gcggtggtag atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag
     8101 aaagtaggag atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct
     8161 agcagaatta ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag
     8221 agtgcgttca aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac
     8281 gatgttccct ccaccaaagg tgttcttatg tagttttaca caggagtctg gacttgacct
     8341 ctagagtcga cctgcgcatc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8401 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8461 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8521 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8581 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8641 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8701 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8761 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8821 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8881 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8941 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9001 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9061 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9121 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9181 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9241 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9301 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9361 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9421 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9481 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9541 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9601 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9661 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9721 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9781 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9841 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9901 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9961 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10021 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10081 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10141 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10201 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10261 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10321 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttctcatgtt
    10381 tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc
    10441 agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
    10501 ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
    10561 gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
    10621 caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
    10681 ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
    10741 ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
    10801 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
    10861 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg act
//
