LOCUS       sequence-179929-        5608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid containing mouse Tusc5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Fazakerley DJ, Naghiloo S, Chaudhuri R, Koumanov F, Burchfield JG, 
            Thomas KC, Krycer JR, Prior MJ, Parker BL, Murrow BA, Stockli J, 
            Meoli CC, Holman GD, James DE
  TITLE     Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumor 
            Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin 
            Action in Adipocytes.
  JOURNAL   J Biol Chem. 2015 Sep 25;290(39):23528-42. doi: 
            10.1074/jbc.M115.657361. Epub 2015 Aug 3.
  PUBMED    26240143
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.657361"; journalName: "J Biol Chem"; date: 
            "2015-09-25- 25"; volume: "290"; issue: "39"; pages: "23528-42"
COMMENT     Sequence Label: pBABE puro Tusc5
FEATURES             Location/Qualifiers
     source          1..5608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..423
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     353..375
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     391..407
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      458..467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1003..1023)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1008..1337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1188..1323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1347..1946
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1347..1366)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1843..1863
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2167..2636
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2766..2783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2937..3525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3017..3036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3685..4476,4477..4545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4308..4327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4546..4650)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4681..5150
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5215..5414
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 aattagggcc agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc
       61 gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc
      121 tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac
      181 ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag
      241 gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc
      301 tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa
      361 cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc
      421 gccggccgga tcccagtgtg gtggtacgta ggaattcgcc accatggcca acccagcgca
      481 gcctccgctt caggacccag gctccacctc acccttggaa ctgcctgaga tggagaaact
      541 actcacaaaa gtggagaaca aggatgacca agccctgaat ctgtccaagt ctctgtcagg
      601 ggctctggac ctggagcaga atggtcacag cctgcccttc aaggtcatct ccgaggggca
      661 ccgccagccc tccctttccg gctccccttc ccgggtcagc tctcggcgag catcctccgt
      721 catcaccaca tcctacgccc aggaccaaga agcccccaaa gattacctgg tccttgccat
      781 tgcctcttgc ttctgccccg tctggcccct caacctcatc ccccttatct tttctatcat
      841 gtctcgaagt agtgtgcagc agggggacct ggatggggca cggaggctgg gccgcctggc
      901 acggctactc agcatcacct tcatcatcct gggcatcgtt atcatcattg tggctgtcac
      961 tgtcaatttc acagttccga agtaagaatt cgtcgaccct gtggaatgtg tgtcagttag
     1021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1081 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1141 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1201 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1261 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1321 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     1381 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     1441 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     1501 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     1561 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     1621 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     1681 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     1741 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     1801 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     1861 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     1921 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     1981 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     2041 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     2101 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     2161 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     2221 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     2281 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     2341 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     2401 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     2461 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     2521 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     2581 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     2641 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     2701 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2761 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2821 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2881 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2941 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3001 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3061 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3121 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3181 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3241 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3301 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3361 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3421 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3481 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3541 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3601 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat
     3661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcagc
     4681 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     4741 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4801 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     4861 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     4921 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     4981 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     5041 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5101 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     5161 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
     5221 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     5281 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     5341 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     5401 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     5461 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     5521 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     5581 tctgactgtg tttctgtatt tgtctgaa
//
