LOCUS       sequence-17994-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-22b DT 51E/148K
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gaggcttttg
       61 atttcaaaaa atagcgatag cttagaatta accttggtgt gatctactgt tttctggtac
      121 ccaagaacgc ctatggaatc cgacgaaatt tcattagaat gaattttctc cgagctgctt
      181 ctgtgaaatg ccacgtgaag attcgcatgc acaccattac caacataaac aggagattta
      241 gggcgacaaa aagttacatc accgtctata gctctgcaac gcatccttat tttccgacca
      301 tttacggaaa tatgagtctt ggacttatta acgtccagct ttccaggaat agtcggtagt
      361 aggacacccg cgattggaag cggggtattt tcagcagtaa tttttatgtc gtgcccactc
      421 tccccttgaa aaccagttcg gattatcgaa tcttcaacag tgttccaact gacagcatac
      481 ccgtcatgaa gaaatggttg cgttttatgc cccggagaat acgcgggacg attatacgaa
      541 ttatgaacta cttgaaataa attgataata ctctctacaa aattatatgc agcgaaacca
      601 atatcaacta gctctcctac caatggaata gcttgagcaa ccattaaaga cgataaagct
      661 attgattgtg ccactatctc ttctgtattg tggtgaacgg caccgtctgc aatgcccatt
      721 acgctaccga taccaggaag tatcgaaaga gcagcagttg tcttttcca
//
