LOCUS       sequence-17995-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-22b DT 51E/148K
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     35..100
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaaa tacctgctgc cgaccgctgc
       61 tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc atggatatcg gaattaattc
      121 ggatccgggc gctgatgatg ttgttgattc ttctaaatct tttgtgatgg aaaacttttc
      181 ttcgtaccac gggactaaac ctggttatgt agattccatt caaaaaggta tacaaaagcc
      241 aaaatctggt acacaaggaa attatgacga tgattgggag gggttttata gtaccgacaa
      301 taaatacgac gctgcgggat actctgtaga taatgaaaac ccgctctctg gaaaagctgg
      361 aggcgtggtc aaagtgacgt atccaggact gacgaaggtt ctcgcactaa aagtggataa
      421 tgccgaaact attaagaaag agttaggttt aagtctcact gaaccgttga tggagcaagt
      481 cggaacggaa gagtttatca aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct
      541 tcccttcgct gaggggagtt ctagcgttaa gtatattaat aactgggaac aggcgaaagc
      601 gttaagcgta gaacttgaga ttaattttga aacccgtgga aaacgtggcc aagatgcgat
      661 gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
//
