LOCUS       sequence-179953-        7106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses transmembrane domains of syntaxin17 tagged with SECFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Itakura E, Kishi-Itakura C, Mizushima N
  TITLE     The hairpin-type tail-anchored SNARE syntaxin 17 targets to 
            autophagosomes for fusion with endosomes/lysosomes.
  JOURNAL   Cell. 2012 Dec 7;151(6):1256-69. doi: 10.1016/j.cell.2012.11.001.
  PUBMED    23217709
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1256-69"
COMMENT     Sequence Label: pMRXIP SECFP-Stx17TM
FEATURES             Location/Qualifiers
     source          1..7106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    8..365
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             430..846
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     776..798
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     814..830
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    856..1230
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1168..1184
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1262..1264,1265..1267,1268..1981)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGILTLGMDELYK"
     primer_bind     complement(1307..1328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1568..1587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1915..1936
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2298..2864
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2314..2866
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2458..2475)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2685..2704
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2878..3477
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2878..2897)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3374..3394
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3701..4170
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4300..4317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4471..5059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4551..4570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5223..6014,6015..6083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5846..5865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6084..6188)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6256..6274
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        6376..6755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg
       61 attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg
      121 gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc agggacttcg
      181 ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg actctttggt
      241 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc
      301 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc
      361 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
      421 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
      481 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
      541 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
      601 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
      661 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
      721 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
      781 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      841 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
      901 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
      961 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
     1021 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
     1081 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
     1141 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
     1201 gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt aaggatctgc
     1261 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1321 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1381 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1441 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1501 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
     1561 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1621 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1681 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     1741 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     1801 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1861 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     1921 cctgctggag ttcgtgaccg ccgccgggat cttgactctc ggcatggacg agctgtacaa
     1981 gggtggcggt ggctcgggcg gtggtgggtc gggtggcggc ggatctatcg atgaattgaa
     2041 ttcgctggca gctctgcctg tggcaggtgc actcatcggg ggaatggtag ggggtcctat
     2101 tggcctcctt gcaggcttca aagtggcagg aattgcagct gcacttggtg gtggggtgtt
     2161 gggcttcaca ggtggaaaat tgatacaaag aaagaaacag aaaatgatgg agaagctcac
     2221 ttccagctgt ccagatcttc ccagccaaac tgacaagaaa tgcagttaag gatccctcga
     2281 gcggccgcta cgtaaattcc gccccccccc ctaacgttac tggccgaagc cgcttggaat
     2341 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2401 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2461 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2521 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2581 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2641 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2701 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2761 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     2821 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     2881 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     2941 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     3001 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     3061 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     3121 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     3181 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     3241 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     3301 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     3361 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     3421 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     3481 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctcgacgata
     3541 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     3601 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     3661 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     3721 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     3781 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     3841 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     3901 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     3961 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     4021 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     4081 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     4141 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     4201 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     4261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5161 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     5221 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5281 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5341 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5401 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5461 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5521 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5581 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5641 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5701 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5761 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5821 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     5881 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5941 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6001 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6061 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6121 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6181 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     6241 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcactc gaggagcttg
     6301 gcccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
     6361 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     6421 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     6481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     6541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     6601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     6661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     6721 agtacatcta cgtattagtc atcgctatta ccatgctaga gaaccatcag atgtttccag
     6781 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     6841 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     6901 ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     6961 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     7021 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     7081 tgcccaggga ccaccgaccc accacc
//
