LOCUS       sequence-179954-        8979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Mi D, Li Z, Lim L, Li M, Moissidis M, Yang Y, Gao T, Hu TX, Pratt T,
            Price DJ, Sestan N, Marin O
  TITLE     Early emergence of cortical interneuron diversity in the mouse 
            embryo.
  JOURNAL   Science. 2018 Feb 22. pii: science.aar6821. doi: 
            10.1126/science.aar6821.
  PUBMED    29472441
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Feb 22. pii: science.aar6821. doi: 10.1126/science.aar6821."
COMMENT     Sequence Label: RV-Cag-Dio-EYFP-P2A-cMaf
FEATURES             Location/Qualifiers
     source          1..8979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             719..894
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    957..1156
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1357..1773
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1703..1725
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1741..1757
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1787..2166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2169..2444
                     /label=chicken beta-actin promoter
     intron          2445..3453
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3377..3399
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(3523..3556)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3607..3640)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4201..4218)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      4758..4767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4777..4833)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(4849..5559,5560..5562,5563..5565))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4891..4912)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5240..5259
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5499..5520
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5562..5571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5583..5616
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5667..5700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5710..6298
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5763..5783)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6181..6192)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6371..6964
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7165..7182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7336..7924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7416..7435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8102..8893,8894..8962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8725..8744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatccggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
       61 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      121 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      661 cccattgacg caaatgggcg gtaggcatgt acggtgggag gtctatataa gcagagctca
      721 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      781 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
      841 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
      901 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      961 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     1021 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1081 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1141 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1201 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1261 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1321 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1381 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1441 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1501 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1561 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1621 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1681 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1741 ctttatccag ccctcactcc ttctctaggc gccggaattc gatgtcgaca ttgattattg
     1801 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1861 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1921 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1981 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2041 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2101 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2161 accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     2221 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     2281 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     2341 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     2401 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc
     2461 cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc tgactgaccg
     2521 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     2581 tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa gggctccggg
     2641 agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     2701 agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     2761 tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     2821 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     2881 ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc gagttgctga
     2941 gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc
     3001 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     3061 gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3121 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     3181 atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa
     3241 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     3301 ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg gggacggctg ccttcggggg
     3361 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     3421 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg
     3481 ctgtctcatc attttggcaa agaattcgct agcggatccg gaataacttc gtataggata
     3541 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
     3601 ctcttaataa cttcgtatag catacattat acgaagttat ttaattaact acatgaagaa
     3661 ctcggggctg ctagggttgt cgctggagct gccgttctct ctgaagccgt tggacaccag
     3721 cttttcgtac ttctctttgt aggcgtccct ttcgcgcacg agtctgctga tctcttgctt
     3781 caggtggtcg acctgctgca gaagctggtt cttctcgctt tccagcacgt gtctctgctg
     3841 cactctcttg aatctgcagc tctgggcgta gcctctgttc ttcagggtcc gccttttctg
     3901 cttcagccgg atcacttctt ctttggacac gcctctcagc tgtctgttca gctcgcgcac
     3961 gctcatggtg accagctgct cgtcagagaa tctgtcgtcg aagtgcagtc cgcctgcagc
     4021 atgatgaggg tgaagtgctc cgcctgcacc agctgttcct ccgccacctc cgccgccacc
     4081 gccaccaccg gcagatgcag gtcctccgcc gccagctccg cctgctccgc tagcagaagc
     4141 agaagctccg ccagcagcac ctggagcacc agctgtagga tgatgatggt ggccggcagc
     4201 atggtgatgg tggtggtgat agtgaggagc agcgccagac tgtgcagcag ctgcagcaat
     4261 cacagcagac accacagcag cggcaggtcc catttcctcg ccagatcctc caagagaagc
     4321 accagcacca gcgcctgcag cagcagccag ctgctgtgcg cctctagcat atccatcgaa
     4381 gccgccttgc agctggtggc tgttgctgat cagggcttcc acagcatcct cagggctaaa
     4441 gcccagagcc tcagggttca gctgctgagg gtagcctgtc atccagtagt aatcttccag
     4501 gtgagccttc tgctcgctgc cagagccagg agatggagca gagaagcttg gagaaggtgg
     4561 cacgctgctg caaggtgtgc tcataggggt gctgctcaga gatccgccag cgatcagtct
     4621 gccgcactgg ctgatgattc tgtctgtctc gacgggctct ttcttcactt cgaacttcat
     4681 caggtcgaag tcgttcacgt attccatggc cagagggctt gtaggcaggt cgctgttgtt
     4741 catggccagt tcagatgcca tggtggcctc cggtccaggc ccggggtttt cttcaacatc
     4801 acctgcttgc tttagcagag agaagtttgt ggcgccggat cctctgtact tgtacagctc
     4861 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     4921 cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt tgtcgggcag
     4981 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     5041 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     5101 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     5161 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     5221 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     5281 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     5341 gtagcgggcg aagcactgca ggccgtagcc gaaggtggtc acgagggtgg gccagggcac
     5401 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     5461 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     5521 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cggcgcgccg
     5581 cgataacttc gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac
     5641 tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat
     5701 gtttaaacca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5761 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5821 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5881 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5941 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6001 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6061 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6121 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6181 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6241 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6301 ggaattcgag ctcgagcttg ttaacatcga taaaataaaa gattttattt agtctccaga
     6361 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     6421 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     6481 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     6541 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     6601 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     6661 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6721 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6781 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     6841 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     6901 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     6961 catttccgac ttgtggtctc gctgccttgg gagggtctcc tctgagtgat tgactacccg
     7021 tcagcggggg tcttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     7081 tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7141 tgcgtattgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7741 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8041 aaaatgaagt ttgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8701 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8941 cacggaaatg ttgaatactc atactcttcc tttttcaat
//
