LOCUS       sequence-179966-       11104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (constitutive rtTA and 
            puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 11104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TA-ERP2
FEATURES             Location/Qualifiers
     source          1..11104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..159
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     151..168
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     152..168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     175..194
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        175..193
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   260..322
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    731..749
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    768..786
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    805..823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    842..860
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    879..897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    916..934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    953..971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     993..1017
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1096..1220
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1245..1250,1251..1268,1269..1275)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1329..1988
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1395..1414)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2330..2635
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2514..2533
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2676..2800)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2915..2934)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2980..3035
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3034..3053)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3478..3857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3972..5277
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4314..5245
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(5294..5911,5912..5917,5918..5953,5954..5956,5957..5992,
                     5993..5995,5996..6031,6032..6040)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(6067..6641,6642..6644,6645..6653)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6234..6251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6461..6480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6654..7253
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6654..6673)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7150..7170
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7339..7911
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   8285..8319
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8468..8486)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8507..8523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8507..8523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8520..8542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8531..8547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8555..8561,8562..8579,8580..8585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8600..8621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8738..8755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8909..9497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8989..9008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9668..10459,10460..10528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10291..10310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10529..10633)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(10659..11104,1..10))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10796..10817)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11008..11027
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
       61 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      121 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
      181 gactcactat agggcgaatt ggagctcggt attcacgaca gcaggctgaa taataaaaaa
      241 attagaaact attatttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
      301 tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
      361 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
      421 caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata
      481 aagtaacaaa acttttaaac attctctctt ttacaaaaat aaacttattt tgtactttaa
      541 aaacagtcat gttgtattat aaaataagta attagcttaa cctatacata atagaaacaa
      601 attatactta ttagtcagtc agaaacaact ttggcacata tcaatattat gctctcgtta
      661 attaactcga gggatccaag ctggccgcac gtctccctat cagtgataga gaagtcgaca
      721 cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga
      781 tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt
      841 ctccctatca gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg
      901 tacgtctaga acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
      961 agtgatagag aaggtacccc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     1021 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     1081 cgcggtggcg gcatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
     1141 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     1201 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
     1261 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
     1321 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     1381 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1441 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1501 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     1561 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1621 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1681 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1741 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     1801 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1861 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1921 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1981 gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     2041 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     2101 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     2161 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     2221 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     2281 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     2341 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     2401 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     2461 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     2521 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     2581 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     2641 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
     2701 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
     2761 cattttacgt ttctcgttca gctttcttgt acaaagtggt gatggccgct ctagcgaagg
     2821 gatcccccat cgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     2881 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     2941 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     3001 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3061 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     3121 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     3181 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     3241 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     3301 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     3361 ccctcgacct gcaggaattc gattacttaa gcctaggatc cagatctgta cattcgaatc
     3421 gatgaattcg ttaaccatgg aagcttctgc agcggccgca ctagcggtgg cggcctcgac
     3481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3841 tcatcgctat taccatgatg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttgac
     3961 tagtggagcc gagagtaatt catacaaaag gagggatcgc cttcgcaagg ggagagccca
     4021 gggaccgtcc ctaaattctc acagacccaa atccctgtag ccgccccacg acagcgcgag
     4081 gagcatgcgc ccagggctga gcgcgggtag atcagagcac acaagctcac agtccccggc
     4141 ggtgggggga ggggcgcgct gagcgggggc cagggagctg gcgcggggca aactgggaaa
     4201 gtggtgtcgt gtgctggctc cgccctcttc ccgagggtgg gggagaacgg tatataagtg
     4261 cggtagtcgc cttggacgtt ctttttcgca acgggtttgc cgtcagaacg caggtgagtg
     4321 gcgggtgtgg cttccgcggg ccccggagct ggagccctgc tctgagcggg ccgggctgat
     4381 atgcgagtgt cgtccgcagg gtttagctgt gagcattccc acttcgagtg gcgggcggtg
     4441 cgggggtgag agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg tccggcttga
     4501 ggcctagcgt ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt tttttgtcct
     4561 tgctgccctc gattgccttc cagcagcatg ggctaacaaa gggagggtgt ggggctcact
     4621 cttaaggagc ccatgaagct tacgttggat aggaatggaa gggcaggagg ggcgactggg
     4681 gcccgcccgc cttcggagca catgtccgac gccacctgga tggggcgagg cctgtggctt
     4741 tccgaagcaa tcgggcgtga gtttagccta cctgggccat gtggccctag cactgggcac
     4801 ggtctggcct ggcggtgccg cgttcccttg cctcccaaca agggtgaggc cgtcccgccc
     4861 ggcaccagtt gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca aggagctcaa
     4921 aatggaggac gcggcagccc ggtggagcgg gcgggtgagt cacccacaca aaggaagagg
     4981 gccttgcccc tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc cgcatagtca
     5041 cctcgggctt ctcttgagca ccgctcgtcg cggcgggggg aggggatcta atggcgttgg
     5101 agtttgttca catttggtgg gtggagacta gtcaggccag cctggcgctg gaagtcattc
     5161 ttggaatttg cccctttgag tttggagcga ggctaattct caagcctctt agcggttcaa
     5221 aggtattttc taaacccgtt tccaggtgtt gtgaaagcca ccgctaattc aaagcaatcc
     5281 ggagtaattc accatgtcta gactggacaa gagcaaagtc ataaacggcg ctctggaatt
     5341 actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5401 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     5461 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5521 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
     5581 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5641 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5701 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     5761 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     5821 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     5881 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
     5941 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6001 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcattccgc
     6061 cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     6241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     6301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6541 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6601 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgaccg
     6661 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6721 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6781 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     6841 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6901 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6961 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7021 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7081 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7141 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7201 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     7261 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg aaatgaccga
     7321 ccaagcgaat tcgctaggat tatccctaat acctgccacc ccactcttaa tcagtggtgg
     7381 aagaacggtc tcagaactgt ttgtttcaat tggccattta agtttagtag taaaagactg
     7441 gttaatgata acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt ttgtggacca
     7501 ctttggtttt cttttttgcg tgtggcagtt ttaagttatt agtttttaaa atcagtactt
     7561 tttaatggaa acaacttgac caaaaatttg tcacagaatt ttgagaccca ttaaaaaagt
     7621 taaatgagaa acctgtgtgt tcctttggtc aacaccgaga catttaggtg aaagacatct
     7681 aattctggtt ttacgaatct ggaaacttct tgaaaatgta attcttgagt taacacttct
     7741 gggtggagaa tagggttgtt ttccccccac ataattggaa ggggaaggaa tatcatttaa
     7801 agctatggga gggttgcttt gattacaaca ctggagagaa atgcagcatg ttgctgattg
     7861 cctgtcacta aaacaggcca aaaactgagt ccttgggttg catagaaagc tgccaaacgc
     7921 tagccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc atcgtctaaa
     7981 gaactaccca ttttattata tattagtcac gatatctata acaagaaaat atatatataa
     8041 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt
     8101 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa
     8161 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag
     8221 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag
     8281 aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat ttgcgcttta
     8341 ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca cattgtggcc
     8401 aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga ggtaccagct
     8461 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
     8521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10621 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
    10681 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
    10741 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
    10801 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
    10861 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
    10921 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
    10981 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    11041 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    11101 gcta
//
