LOCUS       sequence-179974-        6629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses syntaxin17 lacking N-terminal domain tagged with Myc in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N
  TITLE     Accumulation of undegraded autophagosomes by expression of 
            dominant-negative STX17 (syntaxin 17) mutants.
  JOURNAL   Autophagy. 2017 Aug 3;13(8):1452-1464. doi: 
            10.1080/15548627.2017.1327940. Epub 2017 Jun 9.
  PUBMED    28598244
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2017.1327940"; journalName: "Autophagy"; date: 
            "2017-08-3- 3"; volume: "13"; issue: "8"; pages: "1452-1464"
COMMENT     Sequence Label: pMRXIP 3xMyc-STX17-Delta-NTD
FEATURES             Location/Qualifiers
     source          1..6629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..428
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     358..380
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     396..412
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    438..812
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     750..766
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             832..861
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             868..897
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             904..933
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1419..1971
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1563..1580)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1790..1809
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1983..2582
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1983..2002)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2479..2499
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2806..3275
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3405..3422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3576..4164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3656..3675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4328..5119,5120..5188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4951..4970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5189..5293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5361..5379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        5481..5860
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6219..6576
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 atatgggccc gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
       61 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
      121 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
      181 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
      241 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
      301 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
      361 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      421 taggcgcccc catatggcca tatgagatct tatatggggc acccccgccc cttgtaaact
      481 tccctgaccc tgacatgaca agagttacta acagcccctc tctccaagct cacttacagg
      541 ctctctactt agtccagcac gaagtctgga gacctctggc ggcagcctac caagaacaac
      601 tggaccgacc ggtggtacct cacccttacc gagtcggcga cacagtgtgg gtccgccgac
      661 accagactaa gaacctagaa cctcgctgga aaggacctta cacagtcctg ctgaccaccc
      721 ccaccgccct caaagtagac ggcatcgcag cttggataca cgccgcccac gtgaaggctg
      781 ccgaccccgg gggtggacca tcctctagac tgccggatca tcgccatggg tgaacaaaag
      841 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      901 ggtgaacaaa aattaatctc agaagaagac ttgaacgctc gagaattggg gaattcacgc
      961 gttcgcgagt taacggatcc gattcctcaa gatcaaaatg ctgcagaatc gtgggaaacc
     1021 ttagaagcgg acttaattga acttagccaa ctggtcactg acttctctct cctagtgaat
     1081 tctcagcagg agaagattga cagcattgca gaccatgtca acagtgctgc tgtgaatgtt
     1141 gaagagggaa ccaaaaactt agggaaggct gcaaaataca agctggcagc tctgcctgtg
     1201 gcaggtgcac tcatcggggg aatggtaggg ggtcctattg gcctccttgc aggcttcaaa
     1261 gtggcaggaa ttgcagctgc acttggtggt ggggtgttgg gcttcacagg tggaaaattg
     1321 atacaaagaa agaaacagaa aatgatggag aagctcactt ccagctgtcc agatcttccc
     1381 agccaaactg acaagaaatg cagttaagga tccccctaac gttactggcc gaagccgctt
     1441 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     1501 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     1561 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     1621 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     1681 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     1741 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     1801 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     1861 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     1921 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     1981 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     2041 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     2101 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     2161 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     2221 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     2281 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     2341 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     2401 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     2461 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     2521 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     2581 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catggctcga
     2641 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     2701 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     2761 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     2821 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     2881 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     2941 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     3001 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     3061 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     3121 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     3181 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     3241 tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt
     3301 tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa
     3361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4261 acctagatcc ttttgcggcc ggccgcaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5341 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cactcgagga
     5401 gcttggccca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
     5461 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     5521 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5581 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5641 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5701 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     5761 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     5821 ttggcagtac atctacgtat tagtcatcgc tattaccatg ctagagaacc atcagatgtt
     5881 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     5941 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6001 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     6061 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     6121 gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag
     6181 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg
     6241 tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta
     6301 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc
     6361 cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc
     6421 caaaaatccc gatcgttttg gactctttgg tgcacccccc ttagaggagg gatatgtggt
     6481 tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt
     6541 ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg
     6601 tctgactgtg tttctgtatt tgtctgaaa
//
