LOCUS       sequence-179975-        7308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses syntaxin17 lacking N-terminal domain tagged with GFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Uematsu M, Nishimura T, Sakamaki Y, Yamamoto H, Mizushima N
  TITLE     Accumulation of undegraded autophagosomes by expression of 
            dominant-negative STX17 (syntaxin 17) mutants.
  JOURNAL   Autophagy. 2017 Aug 3;13(8):1452-1464. doi: 
            10.1080/15548627.2017.1327940. Epub 2017 Jun 9.
  PUBMED    28598244
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2017.1327940"; journalName: "Autophagy"; date: 
            "2017-08-3- 3"; volume: "13"; issue: "8"; pages: "1452-1464"
COMMENT     Sequence Label: pMRXIP-GFP-STX17-Delta-NTD
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..370
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             435..851
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     781..803
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     819..835
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    861..1235
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1173..1189
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1247..1249,1250..1252,1253..1963)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1247..1249,1250..1252,1253..1963)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1292..1313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1553..1572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1900..1921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2505..3071
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2521..3073
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2665..2682)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2892..2911
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3085..3684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3085..3104)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3581..3601
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3908..4377
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4507..4524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4678..5266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4758..4777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5430..6221,6222..6290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6053..6072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6291..6395)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6463..6481
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        6583..6962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
       61 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
      121 ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga
      181 cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct
      241 ttggtgcacc ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac
      301 agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc
      361 ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
      421 aaaatatggg cccgggccag actgttacca ctcccttaag tttgacctta ggtcactgga
      481 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
      541 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
      601 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
      661 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
      721 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
      781 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
      841 ctctaggcgc ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa
      901 acttccctga ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac
      961 aggctctcta cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac
     1021 aactggaccg accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc
     1081 gacaccagac taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca
     1141 cccccaccgc cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg
     1201 ctgccgaccc cgggggtgga ccatcctcta gactgccgga tctgccatgg tgagcaaggg
     1261 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1321 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1381 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1441 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1501 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1561 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1621 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1681 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1741 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1801 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1861 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1921 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtggcg gtggctcggg
     1981 cggtggtggg tcgggtggcg gcggatctat cgatgaattg aattcacgcg ttcgcgagtt
     2041 aacggatccg attcctcaag atcaaaatgc tgcagaatcg tgggaaacct tagaagcgga
     2101 cttaattgaa cttagccaac tggtcactga cttctctctc ctagtgaatt ctcagcagga
     2161 gaagattgac agcattgcag accatgtcaa cagtgctgct gtgaatgttg aagagggaac
     2221 caaaaactta gggaaggctg caaaatacaa gctggcagct ctgcctgtgg caggtgcact
     2281 catcggggga atggtagggg gtcctattgg cctccttgca ggcttcaaag tggcaggaat
     2341 tgcagctgca cttggtggtg gggtgttggg cttcacaggt ggaaaattga tacaaagaaa
     2401 gaaacagaaa atgatggaga agctcacttc cagctgtcca gatcttccca gccaaactga
     2461 caagaaatgc agttaaggat ccctcgagcg gccgctacgt aaattccgcc ccccccccta
     2521 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     2581 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     2641 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     2701 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     2761 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     2821 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     2881 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     2941 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     3001 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3061 cacgatgata atatggccac aaccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3121 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3181 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     3241 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     3301 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     3361 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     3421 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     3481 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     3541 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     3601 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     3661 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     3721 agcgcacgac cccatggctc gacgataaaa taaaagattt tatttagtct ccagaaaaag
     3781 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     3841 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     3901 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     3961 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     4021 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     4081 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     4141 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     4201 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     4261 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     4321 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat
     4381 gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata
     4441 gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4501 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4561 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4621 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5101 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5341 gagattatca aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa
     5401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     6061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6421 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6481 ccctttcgtc ttcactcgag gagcttggcc cattgcatac gttgtatcca tatcataata
     6541 tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta
     6601 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     6661 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     6721 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     6781 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6841 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6901 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     6961 tgctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     7021 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     7081 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg
     7141 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     7201 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     7261 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgaccca
//
