LOCUS       sequence-179980-       10706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: 6His-MBP-TEV-huAsCpf1
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1334..1525
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1519..1541)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1627..1766
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1682..1701
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1781..1798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2032..2051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2711..3502,3503..3571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3334..3353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3572..3676)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3702..4157)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3839..3860)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4051..4070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(4194..4241)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4237..4255
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4308..4325)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4341..4385)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(4386..4409)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(4434..4514)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(4521..4568)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4569..8489)
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4840..4849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(8490..8510)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(8517..8534)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      8687..8696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9651..9668)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             9682..9704
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    9719..9743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(9743..9762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9744..9762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9846..9865)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        10071..10148
                     /gene="lacI"
                     /label=lacI promoter
     CDS             join(10149..10706,1..525)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(10168..10187)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
       61 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
      121 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
      181 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
      241 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta
      301 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
      361 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
      421 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      541 tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa
      601 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
      661 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
      721 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
      781 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
      841 ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc tgtcgttgag
      901 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
      961 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     1021 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
     1081 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     1141 aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
     1201 cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     1261 cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta
     1321 cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
     1381 aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
     1441 catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
     1501 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     1561 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     1621 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     1681 gcggcatcag agcagattgt actgagagtg caccattgcg gtgtgaaata ccgcacagat
     1741 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2821 catctggccc cagtgctgca atgataccgc gactaccacg ctcaccggct ccagatttat
     2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3001 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     3721 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3781 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3841 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3901 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3961 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4021 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4081 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4141 gccgctacag ggcgcgtccc attcgccaat ccggatatag ttcctccttt cagcaaaaaa
     4201 cccctcaaga cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca
     4261 gcagccaact cagcttcctt tcgggctttg ttagcagccg gatctcagtg gtggtggtgg
     4321 tggtgctcga caagctttta ctcgtgccat tcaattttct gggcttcaaa gatatcgttc
     4381 aggcctttat catcatcatc tttataatcg ctaccaccac cctcgaggct ttaggcatag
     4441 tcggggacat catatgggta tgcataatca ggcacgtcgt agggataagc gtaatctgga
     4501 acatcgtatg ggtaggatcc ctttttcttt tttgcctggc cggccttttt cgtggccgcc
     4561 ggccttttgt tgcgcagctc ctggatgtag gccagccagt cctgattgga gatgccgttc
     4621 tgcagcttca gatccttgct ctccttcagg tgattcagca gcagctggcc cttcagggcg
     4681 atgtggtagg cgccattggc atcggcgtcc atgggccact ctgggttctg aaaccgggag
     4741 tcgaagcaca cgccattcag atcgcgcacg gggctgttga tatagtcctc gcctgtggcg
     4801 gcattggagt tccgcatctg cagcacgctg cggatcaggg ccaccatggt gtcgatggcg
     4861 tgagaatcgt cattctccag cagctttggc aggatgttgg agccatccct gaacacgatg
     4921 cccttctcct ccagcagggc gatcagctcg ttggcaggat acaggtcccg gtatctgccg
     4981 gtgaatctgt gattctcgat cactggcacg attctcttgc cggcgatgaa aggggtgccc
     5041 ttggcgtcaa actgtgtctc gttcttctcg aacacgatat cccatgcagg cataaagccg
     5101 ggcaggcccc tctggaagga cagatttctg ttcatcttaa agtgcaggat gaagtcgccg
     5161 gttttcacgt cgtagtgcag aaagtcgaag ccctccagga agtgcttgcg gctctcgtga
     5221 ttcttgatgg ttttccacac gaaggggtcc acgaagccgg tcaggggatc gatcttagat
     5281 gtatatgggg caggcacgta aaacaggaag ccagactggg tgcccatctt ggcaaaggag
     5341 gtgaactggt ctgtcagctg gtatgggttc agcacgcctc ccactttctc tgctggatag
     5401 tccttcagca ccaggcaatt cagcttatcg atcagcatct tctcgaactg ctggtacacg
     5461 gccttctcgg cgatgccggt cctcttgctc ttaaagccga aattcaggtt ctccagcacc
     5521 accacggcct ggtagtggat catcaggtcc acgatctcgt ggatgacctg gctcagatag
     5581 ccctgcttca gatccttgat tgtgcccacc acagaccagg cctgccttgc tgccaccctc
     5641 tccttctccc tgttgtccag cttcttctgg taatcaaact gctggatggt gttcaggctc
     5701 cgctgctcca ggatcttgcc ggtggagtcg atcactgtga tatagatcag gtttctctcg
     5761 ccccgatcga tgccgatgat aggtgtctcg gggtgctcct tcaggtaggc attcaccctc
     5821 tggttgaact tagatgggga attggcggcc tgatagttca gtgtgatagg cacgtggaaa
     5881 aagaacttgt cgctggtaaa gcgcctatcc ttgatgatct cgtgagacac ctccttggtg
     5941 atcacgttgg gcagcagggc cctggcctca tcagacaggt cgtgggacag tctgtgattc
     6001 acatagtcgt acagctcctg gtacagggtg tcggggattg gggttttctg atccttcagc
     6061 ttcttgttca gcatcttctc tcccagccgg tgtgccatcc tcttcatcct ggacttaggg
     6121 cggtagaaca gctcggcctg gccattcagc ttgatgcttg tcttggccag gttctctgga
     6181 gaaaacaggc cggtccaata cagtgtgtgc agattaggct tgccgtggtg gcccttggca
     6241 aagtccttgt tatagatctg gaacaggtac agcttgcctg tctccacggc atccatgatc
     6301 tccttctcgg cgattctctg gaagctgatg tggtacagca ggggattcag ctcggcatag
     6361 tactcgccca ggtccttata ctgagaggat ggccgcaggc tagacagatc gatagaggtt
     6421 gtcttggtat acttggacag aaaatccctt gtgaagtcga tccacttgca cagggcctct
     6481 ctgtagccct tctggtcgcc ggttttcttg gcgtaggctg tctgaaactt ctttggctcc
     6541 ttctcaggat tgttcaggtc gtagatctcc tttgtgatct ccagaggctc gatgaaattg
     6601 ttggacagca ggatgggggt tgtgtgggtc tgaaagtggg ctgtcacggc cttcagctgg
     6661 gtgctgcact ttgggatcat cttggcggca tcagggaagt agtcatagta catcttatca
     6721 aagccctcgc tggttttctc tgtgggctcg aagctcaggg ccttatacct gcccttctgc
     6781 tttggcatga tgcccagata gtacaggccg ttcttcacaa acaggatggc gccattgttc
     6841 ttctccttat tcacgtccca gccagaggcc agtgtaggca tctgaaagtt cagcttgaac
     6901 ttctccacgg agtagggctt cttggtggca taatttctgg ccttgttgta gaagctcaga
     6961 gaaggctcca tctccagctt gatgccggtc agccgggcag agaactcggg gtccacctcg
     7021 ttggactcat ccacggcaaa ccagtccagc aggtggtaca ggcccagcag gctgtccagc
     7081 tgagacttca ggatctcctt ctcctcctgc ttcttcaggg ttgtaggcag tggctgatcc
     7141 agggcggcgt gtgcgtggga caggatctcg ctggttttct gcttgaaggc ctcgctcagc
     7201 tccttgcctg cggcagagat gatctcctgc aggttgatat cctcgtgctt caggctgcgc
     7261 tgcaccttct ccttggcaga cttggtgatc ttgcctgtca gctcggagat tctccgctca
     7321 tacagggcat tcctcagtgt atcccagtgg tcgcacaggg cgctgctgat tgtctccagc
     7381 ttcttgtggc tgatgaagat gtgtgtcagg tcgatgctgt tcagctcgtt aaacagggcc
     7441 tcggctgtct ccagcacgtt ctcgtttctc agcagtgtct tgtacttgca gaaggactgg
     7501 atcacttcct cgtcgctctt aaactcctcc aggatgaaag acagggtgtt cctatcggac
     7561 aggatctgct taaacagggg gatgaatctg tgtggcaggg aggcgatgat gtgggctgtc
     7621 tcatcattct tctggatggc cagattcagc acctcgttca ggcccttgat cttctcggtg
     7681 cctgcctccc gagagattcc tcccagcagc tggttataca ggtcgatctg ggtctgtgtc
     7741 agcagctggt tataaaaagg gaaggaaaac acctcctcga tggaggtgct cacgaagatg
     7801 ccgatggcct tcttcacgtt ctcaaagtgc tcccgcaggc tgggcacggc ggtgatcagg
     7861 cgtgtgaaga tgtgacaatt ctccttaaac ttggggaagt tgtcctgcac gatgcggtgt
     7921 gggatggctg tgctgatatc ctcggcgctg aacacgttct tcctgttctc ataaaagccg
     7981 gagaagtagg ttgtaaactt gtcgaagctc cgcagcaggg cgttctcgtg ctcggttgtg
     8041 gtcacggtgc ccagctgctt cagcaccttg ccattaaaca gctcggcctt gaacaggccc
     8101 ttgtagatct cggcgtgtct cttattgatg gcatcggtca ggttgtctgt ccggccgatg
     8161 aagtagtcgt ggatggcatt gcgatatgtg gcctgctcct cgatcagggc gttccttgtc
     8221 tcctcggttt tctcctttct ataggagtcg atggcggcgc tcaggttctc ccaatccagc
     8281 tgcaccagct gcaggcactg gtcggcatag gtcttgtaga tccgatcgat gatgggcttc
     8341 agctccttgt agtgatcatt gcgggccttg tcctcctcga tgaagccctg ctcctggatg
     8401 tgcttcaggg tcttgccctg tgggatcagc tcaaaccgca gtgtcttgct cacctgatac
     8461 aggttggtaa agccctcgaa ctgtgtcatt ccctgaaaat acagattttc gctagcgcta
     8521 ccacgcggaa ccagggatcc ggtgcttgca ccggtctggg catctttcag ggcttcatca
     8581 acggtctgac gacctgatgc ggcattaatc actgcggtac gaacggcata ccaaaatgcg
     8641 ctcatctgcg gaatattcgg cataatttcg cctttctgtg cattttccat ggtggctgca
     8701 atacgcggat ctttaaccag ttcttcttca tagcttttca gtgcaactgc acccagcggt
     8761 ttatctttat tcactgcttc cagaccttca tcggtcagca gatagttttc cagaaattct
     8821 tttgccagtt ctttgttcgg gcttgctgca ttaatacctg cgctcagaac accaacaaac
     8881 ggtttgctcg gctgaccttt aaatgtcggc agaacggtaa caccataatt cactttgctg
     8941 gtatcaatat ttgaccatgc ccacggacca ttaattgtca ttgcggtttc acctttgtta
     9001 aatgctgctt ctgcaatgct ataatcggta tcggcgttca tgtgtttgtt tttaatcaga
     9061 tcaaccagaa aggtcagacc ggcttttgca cccgcattat caacacccac gtctttgata
     9121 tcatatttgc cgttttcata tttgaatgca tagccaccat ctgctgcaat cagaggccag
     9181 gtaaaatacg gttcttgcag attaaacatc agtgcgcttt taccttttgc tttcagttct
     9241 ttatccagtg ccggaatttc ttcccaggtt ttaggcggat tcggcagcag atctttgtta
     9301 tagatcaggc tcagtgcttc aactgcaatc ggatatgcaa tcagtttacc gttatagcga
     9361 actgcatccc aggtaaacgg atacagtttg tcctgaaatg ctttatccgg tgtaatttct
     9421 gccagcagac cgctctgtgc ataaccacca aaacgatcat gtgcccaaaa gataatatcc
     9481 ggaccatcac cggttgctgc aacctgcgga aatttttctt ccagtttatc cggatgttca
     9541 acggtcactt taatgccggt atctttttcg aattttttgc caacttctgc cagaccatta
     9601 tagcctttat cgccattgat ccaaataacc agtttgcctt cttcgatttt gtgatggtga
     9661 tgatgatgac ccatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
     9721 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
     9781 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     9841 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     9901 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     9961 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
    10021 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg
    10081 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
    10141 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
    10201 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
    10261 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
    10321 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
    10381 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
    10441 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
    10501 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
    10561 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
    10621 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
    10681 gtcaccagca aatcgcgctg ttagcg
//
