LOCUS       sequence-179983-        7363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing mouse Blimp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-Blimp1
FEATURES             Location/Qualifiers
     source          1..7363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     RBS             3170..3178
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(4385..4404)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4450..4505
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4504..4523)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4866..4882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4866..4882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4879..4901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4890..4906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4914..4920,4921..4938,4939..4944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4959..4980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5039..5234
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5085..5220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5240..5374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5277..5296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5331..5350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5442..5459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5613..6201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5693..5712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6372..7163,7164..7232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6995..7014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7233..7337)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcat gagagaggct
     1741 tatctcagat gttggatctt ctcttggaaa aacgtgtggg tacgaccttg ccaaaggctg
     1801 cattttaaaa ccgtgcttct tcaaggcagt ctactttaca cggctttgga ctcttactca
     1861 actgtacaag ctgcccccaa gtctagctcc ggctccgtga agtttcaagg actggcagag
     1921 actgggatca tgaaaatgga catggaggac gctgatatga ctttgtggac agaggccgag
     1981 tttgaagaga agtgtacata catagtgaac gaccacccct gggattccgg cgctgacggg
     2041 ggtacttctg ttcaagccga ggcatcctta ccaaggaacc tgcttttcaa gtatgctgcc
     2101 aacaacagca aagaggttat tggcgtggta agtaaggagt acataccgaa gggaacacgc
     2161 tttggacccc tcatcggtga agtctacact aatgacacag ttcccaagaa tgccaacagg
     2221 aagtattttt ggcggatcta ttccagagag gagttccacc acttcattga tggctttaat
     2281 gaggagaaaa gcaactggat gcgctacgtg aatccagctc actctgcccg ggagcaaaac
     2341 ctggctgcct gtcagaacgg gatgaacatc tacttctaca ctattaagcc tatccctgcc
     2401 aaccaggaac ttcttgtgtg gtattgtcgg gactttgcgg agaggctcca ctacccttat
     2461 cctggagagc tcacagtgat aaatctcaca caaacggaaa gcaacccaaa gcaatacagt
     2521 agtgagaaaa atgaactcta cccaaagagt gtccccaaga gagagtacag cgtgaaagaa
     2581 attctaaaac tggactccaa tccctccaaa aggaaggaca tctaccgttc caacatttca
     2641 cccttcactt tagaaaagga catggatggc tttcggaaaa atgggagccc cgacatgccc
     2701 ttctaccctc gggtggttta tcctatccgg gcacctctgc cagaagactt tttgaaagcg
     2761 tccctggcct atgggatgga gagacccacc tacataactc acagtcccct tccgtcttcc
     2821 acaactccaa gtccccctgc gagcagcagc ccggagcaga gccttaagag ctccagcccc
     2881 cacagcagcc cgggaaacac ggtgtcaccc ctggcgccag gcctcccaga acaccgggac
     2941 tcctactcct acttgaatgt ttcctatggt tccgagggcc tgggctccta ccctggctat
     3001 gcacctgccc cccacctccc accagctttc attccttctt acaatgctca ctaccccaag
     3061 ttcctgttgc caccgtacgg cattagttcc aatggcttga gcaccatgaa caacatcaat
     3121 ggtatcaaca acttcagcct cttccctagg ttgtatcccg tctacagtaa cctccttagt
     3181 ggcagcagcc tgcctcatcc catgctcaat ccagcttccc taccgagttc cctgcctacc
     3241 gatggagccc ggaggctgct tccaccggag caccccaaag aggtgcttat cccagcaccc
     3301 cacagtgcct tctcccttac cggggctgca gccagcatga aggacgagag tagtcccccc
     3361 agcggatctc caacggcggg aactgcagcc acgtcagaac acgtggtaca acccaaagct
     3421 acctcatcag tgatggcggc ccccagcact gacggagcca tgaatctcat taaaaacaaa
     3481 cgaaacatga ctggttacaa gactcttcct taccctctga agaaacagaa tggcaagatc
     3541 aagtatgagt gcaatgtctg tgccaagacg ttcggtcagc tctccaacct gaaggtccac
     3601 ctgagagtgc acagtggaga acggcctttc aagtgccaga cctgcaacaa gggttttact
     3661 cagctcgccc acctgcagaa acactacttg gtacacacag gagagaagcc acatgagtgc
     3721 caggtctgcc acaagagatt tagcagcaca agcaatctca agacccacct tcgattgcat
     3781 tctggagaaa aaccttacca atgtaaggtg tgccctgcca agtttacgca atttgtgcac
     3841 ctgaagctgc acaagcgact gcatacccgg gagcggcctc acaagtgtgc ccagtgtcac
     3901 aagagctaca tccatctctg cagcctcaag gtccacctga agggcaactg ccctgcgggc
     3961 ccagctgctg ggctgccttt ggaggatctg acccgaatca atgaagaaat tgagaggttc
     4021 gacatcagcg acaatgcaga ccgtcttgag gacatggagg acagtgtcga tgtgacctcc
     4081 atggtggaga aggagattct agctgtggtc agaaaagaga aagaagaaac cagtctgaaa
     4141 gtgtctttgc aaagaaacat ggggaacggc ctcctctcct cagggtgcag cctctatgag
     4201 tcatcggacc tgtccctcat gaagttgcct cacagcaacc cactacctct ggtgcctgta
     4261 aaggtcaaac aagaaacagt tgaaccgatg gatccttaag cggccgcact cctcaggtgc
     4321 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     4381 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     4441 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     4501 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     4561 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     4621 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     4681 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     4741 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     4801 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa
     4861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     5041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5161 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5221 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5341 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     5401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
