LOCUS       sequence-17999-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Shoichet SA, Malik TH, Rothman JH, Shivdasani RA
  TITLE     Action of the Caenorhabditis elegans GATA factor END-1 in Xenopus 
            suggests that similar mechanisms initiate endoderm development in 
            ecdysozoa and vertebrates.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Apr 11. 97(8):4076-81.
  PUBMED    10760276
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Apr 11. 97(8):4076-81."
COMMENT     Sequence Label: p029 End-1::EnR
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    86..220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(123..142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     177..196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     225..244
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        225..243
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcattggcgc cgcggccgcg aattaaaaaa cctcccacac ctccccctga acctgaaaca
       61 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      181 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat
      241 agggctgcag aatctagatt aagatcccag agcagatttc tctggattgg catcgctcat
      301 cttggagctc atgggcggcg ggatgacggc attggctgca ctcctgcgca ggcgactctg
      361 cacgatttcc tcgtgtatcc tgggataggc agccggattg aagcagttaa ggaagttggc
      421 ccgttccagc atcatggccg cagtagcggc tgcggcggca gcttgctgtt gccggctaaa
      481 ctccagcaga tccactcttg taaaggcgga gggcgtggca gtctgggagc gattcgcctc
      541 gaaggggcgg aatatgctgg cctggttctc aatcgacttg ccaggcttct ggacatctcc
      601 gaaacgatcg ctcaggatgt tggagatgga aaaggccagc gagggcttgg ccgtcgactg
      661 ctgctggtgg gcgggtggtg gcaggcgtcc gcccgccgac gtatcatcca catccacatc
      721 aatgtcgtca tcctcctccg cctcctcctc ctcgttggtg gtgtgcgtct gattgtggaa
      781 actcatgtca ccgatcacgg a
//
