LOCUS       sequence-180001-        6817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP1.18.1
FEATURES             Location/Qualifiers
     source          1..6817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1833..1850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1839..2063
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2195..2783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2275..2294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3112..3159
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3184..3203
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3391..4179)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3502..3521)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4106..4125
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4214..4571)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4228..4363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4380..4571
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4528..4548
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4631..5422,5423..5491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5254..5273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5492..5596)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5623..6078
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5710..5729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5920..5941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        6090..6469
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6470..6673
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6624..6644
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6718..6737
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6718..6736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaagcttgc catgggttgc tctttctcta tcttcctctt ggctttgcta tcctgtttga
       61 ccatccccgc ttccgcttat gaagtgcaca acgtgtccgg gatgtaccat gttacgaacg
      121 actgctccaa cacaagcatc gtgtatgagg cagcggacgt gatcatgcac acccccgggt
      181 gcgtgccctg cgttcgggag ggtaattcct cccgctgctg ggtagcgctc actcccacgc
      241 tcgcggccag gaacagcagc gtccctacta cgacaatacg acgccacgtc gatttgctcg
      301 ttgggacggc tgctttctgt tccgctatgt acgtggggga cctctgcgga tctgtcttcc
      361 tcgtctccca gctgttcacc ttctcgcctc gccggcatat gacggtacag gactgcaatt
      421 gctcaatcta tcccggccac gtatcaggtc accgcatggc ttgggatatg atgatgaact
      481 ggtcacctac agcagcctta gtggtatcgc agttactccg gatcccacaa gccgtcgtgg
      541 acgtggtggc gggggcccac tggggaatcc tggcgggcct cgcctactac tccatggtgg
      601 ggaactgggc taaggtcttg cttgtgatgc tactctttgc cggcgttgac ggacagaccc
      661 gcacaacggg ggggtcggca gcccaaacca cctatgggct tacatccctc tttagatctg
      721 gaccgtctca gaagatccaa cttgtgaata ccaacggcag ctggcacatc aacagaactg
      781 ccctgaattg taatgactcc ctcaacactg ggttccttgc tgcgctgttt tacgtgcgca
      841 atttcaactc gtccggatgc ccggagcgca tggccagctg tcgccccatc gacacgttcg
      901 atcaggggtg gggtcccatc acttacaccg agcctcacag cctggaccag aggccctatt
      961 gctggcacta cgcacctcaa ccgtgtggta ttgtgcccgc ggcgcaggtg tgtggtccag
     1021 tgtattgctt caccccaagt cctgttgtag tggggacgac cgatcgtttc ggcgccccta
     1081 cgtacacctg gggggagaat gagacggacg tgctgattct gaacaacacg cggccgccgc
     1141 agggcaactg gttcggctgt acatggatga atggcaccgg gtttaccaag acgtgtgggg
     1201 gccccccatg caacatcggg ggggccggca acaacacctt gacctgtccc acggactgct
     1261 tccggaagca ccccgaggct acctacacca gatgtggttc ggggccttgg ttaacaccta
     1321 ggtgcatggt tgactaccca tacaggctct ggcactaccc ctgcactgtc aactttacca
     1381 tcttcaaggt taggatgtat gtggggggcg tggagcacag gctcaccgcc gcatgcaatt
     1441 ggacccgagg agagcgttgc aacttggagg acagggatag atcagagctt agcccgctac
     1501 tactgtctac gacagagtgg caggtattgc cctgttcctt caccacccta ccggctttgt
     1561 ccactggttt gatccatctc caccagaaca tcgtggacgt gcaataccta tacggtatag
     1621 ggtcagcagt tgtctccatc gcaatcaaat gggagtatat cgtgctgctc ttcctcctcc
     1681 tggcggacgc gcgcgtctgc gcctgcttgt ggatgatgct gctggtagcc caggctgagg
     1741 cctaatctag aaactttaag ccgaattctg cagatatcca gcacagtggc ggccgctcga
     1801 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     1861 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1921 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1981 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2041 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagtggcg gtaatacggt
     2101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2761 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2881 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     2941 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     3001 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     3061 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     3121 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     3181 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     3241 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     3301 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     3361 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3421 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3481 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3541 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3601 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     3661 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttgat catcctgatc
     3721 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     3781 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     3841 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     3901 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     3961 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     4021 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4081 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4141 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     4201 ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     4261 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     4321 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     4381 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     4441 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     4501 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
     4561 ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat gagtaaactt
     4621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     5641 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5701 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5761 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5821 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5881 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5941 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6001 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6061 tattaacgct tacaatttac gcgcgcgttg acattgatta ttgactagtt attaatagta
     6121 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6181 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6241 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt
     6301 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6361 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6421 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6481 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6541 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6601 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     6661 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     6721 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag
     6781 ctcggatcca ctagtccagt gtggtggaat tcggctt
//
